Variation in conservation of the cluster for biosynthesis of the phytotoxin phaseolotoxin in Pseudomonas syringae suggests at least two events of horizontal acquisition

Murillo, Jesús; Bardaji, Leire; Fuente, Laura Navarro de la; Führer, M. E.; Aguilera, Selene; Alvarez‐Morales, Ariel

doi:10.1016/j.resmic.2010.10.011

Cited by 26 publications

(34 citation statements)

References 39 publications

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“…phaseolicola and P. syringae pv. actinidae is associated with integrases, which probably mediated its horizontal exchange between these two pathovars (13,20,64). This would suggest that the mbo operon was acquired by illegitimate recombination, a process that was shown to operate during the diversification of effector genes in P. syringae (65) but is predicted to occur with a very low frequency (66), further supporting the single-acquisition-event hypothesis.…”

Section: Figmentioning

confidence: 67%

“…syringae and to contribute to its virulence in tomato plants (8,11). Recently, Murillo et al (13) reported the production of mangotoxin by strains of pathovar avellanae, as well as the simultaneous production of two antimetabolite toxins, mangotoxin and phaseolotoxin, by strain P. syringae pv. syringae CFBP3388.…”

Section: Discussionmentioning

confidence: 99%

“…Mangotoxin was initially detected in strains from P. syringae pv. syringae (11); however, its production was recently observed in strains of pathovar avellanae (13). Recent studies have reported the involvement of the mgo (8,12,14) and mbo (15) operons in mangotoxin production and P. syringae pv.…”

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The Mangotoxin Biosynthetic Operon ( mbo ) Is Specifically Distributed within Pseudomonas syringae Genomospecies 1 and Was Acquired Only Once during Evolution

Carrión

Gutiérrez‐Barranquero

Arrebola

et al. 2013

Appl Environ Microbiol

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c Mangotoxin production was first described in Pseudomonas syringae pv. syringae strains. A phenotypic characterization of 94 P. syringae strains was carried out to determine the genetic evolution of the mangotoxin biosynthetic operon (mbo). We designed a PCR primer pair specific for the mbo operon to examine its distribution within the P. syringae complex. These primers amplified a 692-bp DNA fragment from 52 mangotoxin-producing strains and from 7 non-mangotoxin-producing strains that harbor the mbo operon, whereas 35 non-mangotoxin-producing strains did not yield any amplification. This, together with the analysis of draft genomes, allowed the identification of the mbo operon in five pathovars (pathovars aptata, avellanae, japonica, pisi, and syringae), all of which belong to genomospecies 1, suggesting a limited distribution of the mbo genes in the P. syringae complex. Phylogenetic analyses using partial sequences from housekeeping genes differentiated three groups within genomospecies 1. All of the strains containing the mbo operon clustered in groups I and II, whereas those lacking the operon clustered in group III; however, the relative branching order of these three groups is dependent on the genes used to construct the phylogeny. The mbo operon maintains synteny and is inserted in the same genomic location, with high sequence conservation around the insertion point, for all the strains in groups I and II. These data support the idea that the mbo operon was acquired horizontally and only once by the ancestor of groups I and II from genomospecies 1 within the P. syringae complex.T he bacterial plant pathogen Pseudomonas syringae is ubiquitous in nature and often causes economically important plant diseases. This bacterial species establishes an epiphytic population in association with a plant host's surfaces prior to infection. There are at least 50 pathovars, many of which cause a wide range of plant diseases that exhibit diverse symptoms, such as leaf or fruit lesions, cankers, blasts, and galls (1-7).P. syringae produces several type III effectors and virulence factors, but the role of its toxins is particularly significant during symptom development (1,(8)(9)(10). The current study is focused on mangotoxin, which inhibits the enzyme ornithine N-acetyltransferase (11, 12). Mangotoxin was initially detected in strains from P. syringae pv. syringae (11); however, its production was recently observed in strains of pathovar avellanae (13). Recent studies have reported the involvement of the mgo (8,12,14) and mbo (15) operons in mangotoxin production and P. syringae pv. syringae virulence. The mbo operon is composed of six genes, and all of them are directly involved in mangotoxin production.The development of specific methods for pathogen detection in planta is very important. In this sense, different PCR methods have been developed and improved to detect different P. syringae pathovars. For example, one PCR method used primers that were based on the 16S-23S ribosomal genes to identify strains of P. syring...

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Section: Figmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 99%

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The Mangotoxin Biosynthetic Operon ( mbo ) Is Specifically Distributed within Pseudomonas syringae Genomospecies 1 and Was Acquired Only Once during Evolution

Carrión

Gutiérrez‐Barranquero

Arrebola

et al. 2013

Appl Environ Microbiol

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“…The Pht-PAI appears to be mobile, because a nearly identical genomic island has been found syntenically inserted into the genome of certain strains of the kiwiplant pathogen Ps pv. actinidiae1317. Additionally, homologues of the three putative TRase genes were found in Ps pv.…”

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“…Besides its relevance as a research model and potential biotechnological applications15, the Pht cluster is of practical significance because it is generally used for the specific detection of this important plant pathogen16. The cluster is embedded into the 38 kb putative pathogenicity island Pht-PAI, whose 5′ end is defined by three genes coding for products with similarities to TRases1317. The Pht-PAI appears to be mobile, because a nearly identical genomic island has been found syntenically inserted into the genome of certain strains of the kiwiplant pathogen Ps pv.…”

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confidence: 99%

Four genes essential for recombination define GInts, a new type of mobile genomic island widespread in bacteria

Bardaji

Echeverría

Rodrı́guez-Palenzuela

et al. 2017

Sci Rep

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Integrases are a family of tyrosine recombinases that are highly abundant in bacterial genomes, actively disseminating adaptive characters such as pathogenicity determinants and antibiotics resistance. Using comparative genomics and functional assays, we identified a novel type of mobile genetic element, the GInt, in many diverse bacterial groups but not in archaea. Integrated as genomic islands, GInts show a tripartite structure consisting of the ginABCD operon, a cargo DNA region from 2.5 to at least 70 kb, and a short AT-rich 3′ end. The gin operon is characteristic of GInts and codes for three putative integrases and a small putative helix-loop-helix protein, all of which are essential for integration and excision of the element. Genes in the cargo DNA are acquired mostly from phylogenetically related bacteria and often code for traits that might increase fitness, such as resistance to antimicrobials or virulence. GInts also tend to capture clusters of genes involved in complex processes, such as the biosynthesis of phaseolotoxin by Pseudomonas syringae. GInts integrate site-specifically, generating two flanking direct imperfect repeats, and excise forming circular molecules. The excision process generates sequence variants at the element attachment site, which can increase frequency of integration and drive target specificity.

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Genome Plasticity and Dynamic Evolution of Phytopathogenic Pseudomonads and Related Bacteria

Scortichini

Marcelletti

Ferrante

et al. 2014

Plasticity in Plant-Growth-Promoting and Phytopathogenic Bacteria

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Variation in conservation of the cluster for biosynthesis of the phytotoxin phaseolotoxin in Pseudomonas syringae suggests at least two events of horizontal acquisition

Cited by 26 publications

References 39 publications

The Mangotoxin Biosynthetic Operon ( mbo ) Is Specifically Distributed within Pseudomonas syringae Genomospecies 1 and Was Acquired Only Once during Evolution

The Mangotoxin Biosynthetic Operon ( mbo ) Is Specifically Distributed within Pseudomonas syringae Genomospecies 1 and Was Acquired Only Once during Evolution

Four genes essential for recombination define GInts, a new type of mobile genomic island widespread in bacteria

Genome Plasticity and Dynamic Evolution of Phytopathogenic Pseudomonads and Related Bacteria

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