Variation in flavonoid and antioxidant activities of Pyrrosia petiolosa (Christ) Ching from different geographic origins

Chen, Jianhua; Shan, Ning; Li, Xuan; Wang, Xiang; Zhou, Xiaofang; Bu, Yuanyuan; Li, Liangbo; Huang, Rongshao

doi:10.3389/fpls.2023.1173489

Cited by 5 publications

(1 citation statement)

References 40 publications

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“…Metabolomics analysis provides a new perspective to understand the metabolic variations in traditional herbs grown in different geographical environments, thereby contributing to the identification of high-quality herbs ( Chen et al., 2023 ). The two chemical types and three metabolic profiles identified in this study exhibited a strong correlation with geographical distribution, and our results showed that the volatile oil content of ALR varies continuously among different geographical populations.…”

Section: Discussionmentioning

confidence: 99%

Authenticating the geographic origins of Atractylodes lancea rhizome chemotypes in China through metabolite marker identification

Zhang,

Wang,

Lyu

et al. 2023

Front. Plant Sci.

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IntroductionAtractylodes lancea is widely distributed in East Asia, ranging from Amur to south-central China. The rhizome of A. lancea is commonly used in traditional Chinese medicine, however, the quality of products varies across different regions with different geochemical characteristics.MethodThis study aimed to identify the chemotypes of A. lancea from different areas and screen for chemical markers by quantifying volatile organic compounds (VOCs) using a targeted metabolomics approach based on GC–MS/MS.ResultsThe A. lancea distributed in Hubei, Anhui, Shaanxi, and a region west of Henan province was classified as the Hubei Chemotype (HBA). HBA is characterized by high content of β-eudesmol and hinesol with lower levels of atractylodin and atractylon. In contrast, the Maoshan Chemotype (MA) from Jiangsu, Shandong, Shanxi, Hebei, Inner Mongolia, and other northern regions, exhibited high levels of atractylodin and atractylon. A total of 15 categories of VOCs metabolites were detected and identified, revealing significant differences in the profiles of terpenoid, heterocyclic compound, ester, and ketone among different areas. Multivariate statistics indicated that 6 compounds and 455 metabolites could serve as candidate markers for differentiating A. lancea obtained from the southern, northern, and Maoshan areas.DiscussionThis comprehensive analysis provides a chemical fingerprint of selected A. lancea. Our results highlight the potential of metabolite profiling combined with chemometrics for authenticating the geographical origin of A. lancea.

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Section: Discussionmentioning

confidence: 99%

Authenticating the geographic origins of Atractylodes lancea rhizome chemotypes in China through metabolite marker identification

Zhang,

Wang,

Lyu

et al. 2023

Front. Plant Sci.

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Research on the pharmacognostic characteristics, physicochemical properties and in vitro antioxidant potency of Rosa laxa Retz. flos

Guo,

Zhao,

et al. 2024

Microscopy Res & Technique

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Rosa laxa Retz. is an unexplored Rosaceae plant in Xinjiang, China, and its flower is traditionally used in Kazak to treat the common cold, fever, and epileptic seizures and lessen the effects of aging. In the present study, the pharmacognostic profiles, physicochemical properties, phytochemical characteristics, and in vitro antioxidant potency of Rosa laxa Retz. flos (RLF) were presented. In the pharmacognostic evaluation of RLF, organoleptic characteristics, internal structures, and powder information were observed, and physicochemical parameters, including moisture content, ash, pH value, swelling degree, and extractives were examined. The quantitative analysis of the chemical composition of four different polar extracts of RLF showed that the aqueous part had the highest total triterpene acid, flavonoid, and polyphenol content (4.50 ± 0.04 mg/g, 50.56 ± 0.03 mg/g, and 60.20 ± 0.09 mg/g, respectively). A high‐performance liquid chromatography (HPLC)‐diode array detector (DAD) method was established and the contents of gallic acid, ellagic acid, astragalin, and tiliroside in RLF were determined simultaneously. In the set concentration range, the linear relationship among the four components was good (r > 0.999), the average recoveries were 97.36%–100.54%. The contents of gallic acid, ellagic acid, astragalin, and tiliroside in RLF samples were (9.46 ± 2.31) mg/g, (10.60 ±0.75) mg/g, (1.13 ± 2.50) mg/g, and (1.11 ± 2.65) mg/g, respectively. The types of its secondary metabolites were determined by fluorescence, color reaction by chemical solvent method, and ultraviolet–visible (UV–Vis) spectroscopy. The functional groups of its secondary metabolites were determined by Fourier transform infrared (FTIR) spectroscopy. Results showed that RLF contains a variety of secondary metabolic products, including flavonoids, phenolic acid, glycoside, and organic acid. TLC identification showed it contains ursolic acid, β‐sitosterol, tiliroside, astragalin, isoquercitrin, kaempferol‐3‐O‐rutinoside, gallic acid, and ellagic acid. The in vitro antioxidant activity of different polar parts of RLF was investigated by DPPH, ABTS, and reduction performance experiments. The aqueous extract had the strongest antioxidant capacity, consistent with the high content of triterpene acids, flavonoids, and polyphenolic compounds. These findings will provide critical information for the study of quality standards and medicinal value of RLF and its extracts, justify its usage in traditional medicinal systems, and encourage the use of this plant in disease prevention and treatment. Its phytochemical composition and pharmacological studies need to be explored in future.Research Highlights Optical microscope and scanning electron microscope (SEM) were used to observe the morphology, and microstructure of Rosa laxa Retz. flos (RLF). The physicochemical properties, fluorescence and phytochemical composition of four different polar extracts of RLF were analyzed by UV–Vis and FTIR. Determination of total triterpenic acid, total flavonoids, and total polyphenols in four different polar extracts of RLF by UV spectrophotometry. A high‐performance liquid chromatography (HPLC)‐diode array detector (DAD) method was established and the contents of gallic acid, ellagic acid, astragalin, and tiliroside in RLF were determined simultaneously. TLC confirmed that RLF contains ursolic acid, β‐sitosterol, tiliroside, astragalin, isoquercitrin, kaempferol 3‐rutinoside, gallic acid, and ellagic acid. The in vitro antioxidant activity of RLF was studied by DPPH, ABTS, and reducing ability experiments.

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Antioxidant activity of herbal medicine Pyrrosia lingua evaluated by electron paramagnetic resonance spectroscopy

Quan,

Zhou,

Guo

et al. 2025

Anal. Methods

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Variation in flavonoid and antioxidant activities of Pyrrosia petiolosa (Christ) Ching from different geographic origins

Cited by 5 publications

References 40 publications

Authenticating the geographic origins of Atractylodes lancea rhizome chemotypes in China through metabolite marker identification

Authenticating the geographic origins of Atractylodes lancea rhizome chemotypes in China through metabolite marker identification

Research on the pharmacognostic characteristics, physicochemical properties and in vitro antioxidant potency of Rosa laxa Retz. flos

Antioxidant activity of herbal medicine Pyrrosia lingua evaluated by electron paramagnetic resonance spectroscopy

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