“…Prior to quantification, gangliosides are typically extracted from the complex dairy matrix using mixtures of chloroform/methanol (Moore, Ettinger, & Yokoyama, 2000;Sørensen, 2006) or removal of the neutral lipids using acetone followed by chloroform/methanol extraction (Martin, Martin-Sosa, Garcia-Pardo, & Hueso, 2001a;Moore et al, 2000;Puente et al, 1992;Sanchez Diaz et al, 1997;Takamizawa, Iwamori, Mutai, & Nagai, 1986). Some researchers also include a concentration and purification step after extraction, using techniques such as high-performance liquid chromatography (HPLC), solid phase extraction (SPE) or dialysis (Moore et al, 2000;Sanchez Diaz et al, 1997;Sørensen, 2006). Although this allows for the removal of matrix interferences, the recovery of gangliosides was generally unsatisfactory.…”