Variation in seed proteins from mutagen‐treated cultivars and selected lines of Vigna unguiculata L. Walp

Abstract

The storage protein profiles of the seeds of two IITA cowpea cultivars ('IT84E-124' and 'Vita 7') exposed to three mutagens -sodium azide (NaN3), ethyl methane sulphonate (EMS) and ^''Co gamma rays -and those of 18 selected M3 lines were investigated by sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis (PAGE). The total seed protein, globulin and albumin fractions showed differences in number and intensity of subunit bands, but the least differences were found in the albumin fraction. Both high and low molecular weight bands were observed, the highest being 94.5 kDa and the lowest 12.0 kDa. 'Vita T showed less variability compared with 'IT84E-124', as indicated by the relative similarity indices (S.I.) ofthe two cultivars. The lowest S.I. of 0.200 was found among 'IT84E-124' lines while the lowest S.I. among 'Vita T lines was 0.4706. A number of lines with particular traits were found to be characterized by the presence of specific polypeptide bands. This study demonstrates that induced mutation could create additional variability to supplement existing germplasm and that SDS-PAGE is a useful tool for discriminating and estimating genetic similarities among selections.

Assessment of Genetic Diversity in Bambara Groundnut (Vigna subterranea) Landraces Based on Seed Storage Proteins Electrophoretic Pattern

“…Tabatabei et al (2011) reported that human factors are very important for the association between accessions from different locations. Results within close range of similarity coefficient were similarly reported in many other species; for example, 0.82-0.24 in cowpea cultivars and 0.96-0.47 in sesame accessions (Osanyinpeju and Odeigah, 1998;Alege, 2015).…”

Mutagenic Effects of Gamma Radiation on Eight Accessions of Cowpea (<i>Vigna unguiculata</i> [L.] Walp.)

“…The first mutant cultivar was produced in tobacco in Indonesia in 1936 and up to the recent time, 3220 cultivars in over 220 crop species have been developed and released to farmers all over the world [7]. However, little work has been reported on mutation breeding in cowpea [8]- [10], which has been widely used among other legume crops. An exception is India where 10 cowpea mutant cultivars have been released to farmers [11].…”

Seed coat color, weight and eye pattern inheritance in gamma-rays induced cowpea M2-mutant line

“…In cowpea, it has been reported that irradiation mutations have resulted in changes in morphological and reproductive traits [17], [18]. At the biochemical level, two cowpea cultivars have shown variation in number as well as intensity of protein bands after exposure to gamma rays [19], [20].…”