Variation in speed of infection of “no root hair zone” of white clover and nodulating competitiveness among strains of Rhizobium trifolii

Stephens, P. M.; Cooper, J. E.

doi:10.1016/0038-0717(88)90059-4

Cited by 22 publications

(14 citation statements)

References 17 publications

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“…Our results support those of Bhuyaneswari et al (1981), and Stephens and Cooper (1988) with other legumes, that a delay in inoculation resulted in a downward shift in nodule formation on the taproot. The results of our delayed inoculation experiment (Table 1) indicate that nodulation sites remained susceptible to nodulation for one to two days.…”

Section: Resultssupporting

confidence: 91%

“…Nodulation sites on white clover are limited and available for infection during a finite period (Bhuyaneswari et al, 1981;Stephens and Cooper, 1988) which suggests that, if high rhizobial populations are not present as seedlings develop, nodulation sites near the crown may no longer be susceptible to nodulation by the time the site becomes exposed to rhizobia. Field experimentation with white clover (T. repens L.…”

Section: Introductionmentioning

confidence: 99%

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Number of Rhizobia and delayed inoculation influence nodulation of clovers

Nazih

Weaver

1994

Plant Soil

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The relationship between numbers of rhizobia and nodulation response of legumes is of considerable practical importance. Experiments were done under controlled conditions to determine the influence of numbers of Rhizobium leguminosarum biovar, trifolii on nodulation of arrowleaf clover (Trifolium vesiculosum Savi.) and crimson clover (T. incarnatum L.). Numbers of rhizobia in excess of 1000 per seed did not substantially increase earliness of nodulation or total number of nodules formed on the taproot. Nodules, however, were formed nearer the top of the taproot as numbers of rhizobia increased to 100,000 per seed. Delayed inoculation experiments indicated that nodulation sites for these clovers only remained susceptible to infection for less than 1 day. Delaying inoculation for 4 days resulted in only a 1 to 2 day delay in nodulation for arrowleaf and crimson clovers respectively and no delay for subterranean clover (T. subterraneum L.). Apparently, larger seedlings nodulated faster.

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Section: Resultssupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Number of Rhizobia and delayed inoculation influence nodulation of clovers

Nazih

Weaver

1994

Plant Soil

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“…Their studies delayed inoculation up to a maximum of 8 d in newly developing seedlings. Similar studies performed on white clover (Trifolium repens L.) (Bhuyanewari et al 1981;Stephens and Cooper 1988), arrowleaf clover (T. vesiculosum Savi) and crimson clover (T. incarnatum L.) (Nazih and Weaver 1994) all suggest that numbers of nodulation sites are limited and only available for infection during a finite period early in root development. However, nodule development on the root system of the perennial forage, lucerne (alfalfa) changed dramatically with the age of the stand .…”

mentioning

confidence: 55%

Delayed inoculation of alfalfa with Sinorhizobium meliloti and Penicillium bilaiae

Knight

2015

Can. J. Plant Sci.

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Knight, J. D. 2015. Delayed inoculation of alfalfa with Sinorhizobium meliloti and Penicillium bilaiae. Can. J. Plant Sci. 95: 205Á211. The persistence of perennial forage legume crops relies on the establishment of an effective symbiotic relationship with the appropriate Rhizobium species and strain. Situations can arise where a forage legume crop fails to symbiotically fix N 2 . This study investigates if inoculation of alfalfa with a commercial Sinorhizobium meliloti inoculant 1 yr after seeding can induce biological N 2 fixation at levels similar to those achieved when the inoculant is applied at seeding. Alfalfa (Medicago meliloti cv. Algonkwin) was grown at two sites in Saskatchewan and inoculated with S. meliloti or S. meliloti plus the P-solubilizing fungus Penicillium bilaiae. The inoculants were applied at seeding or applied 1 yr after seeding. Biological N 2 fixation was measured in the fall of the delayed inoculation year as percentage of N derived from atmosphere (%Ndfa) using the 15 N isotope dilution technique. Inoculation with S. meliloti increased %Ndfa at both sites relative to uninoculated and fertilized controls but had no effect on total N content or yield. Inoculating alfalfa the year after seeding increased %Ndfa relative to the controls at both sites, and at one of the sites %Ndfa in the delayed treatments was at the same levels measured in the year of seeding treatments.

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“…Detailed time course analyses of nodule formation by five R. trifolii strains inoculated onto seedlings of Trifolium repens in single culture were obtained by using an agar plate modification of the methods of Bhuvaneswari et al (1980) and Stephens and Cooper (1988). The rooting medium used was that described by Wood (1982) solidified with 0.5% agar.…”

Section: Single-strain Nodulation Profilesmentioning

confidence: 99%

“…Stacey and Upchurch (1984) have suggested that an approach directed towards understanding the events necessary for nodule initiation should provide clues as to which bacterial traits enhance competitive ability. Stephens and Cooper(1988), studying the nodulating competitiveness of paired strains of Rhizobium leguminosarum bv. trifolii, have found that differences between strains with regard to speed of infection of the host plant in single culture influence their competitive relationships when they are presented to the host as a mixed culture.…”

Section: Introductionmentioning

confidence: 99%

Nodulation of Trifolium repens at low pH by single and paired strains of Rhizobium leguminosarum biovar trifolii

Stone

Cooper

1997

Archives of Microbiology

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Detailed individual nodulation profiles were obtained for five strains of Rhizobium leguminosarum biovar trifolii inoculated onto roots of Trifolium repens seedlings growing on an agar medium of pH 4.5. The time of appearance and the location of every nodule were noted for a period of 10 days after inoculation. Using these nodulation frequency profiles, pairings of strains were identified and six mixed-strain inoculation (1:1 ratio) experiments were subsequently performed at pH 4.5. Results from the mixed-inoculum experiments showed that the performance of a Rhizobium strain in single culture could not be reliably used to predict the outcome of a paired-inoculation study and that some seedlings were exclusively nodulated by rhizobia that performed poorly at low pH in single-culture inoculations.

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Variation in speed of infection of “no root hair zone” of white clover and nodulating competitiveness among strains of Rhizobium trifolii

Cited by 22 publications

References 17 publications

Number of Rhizobia and delayed inoculation influence nodulation of clovers

Number of Rhizobia and delayed inoculation influence nodulation of clovers

Delayed inoculation of alfalfa with Sinorhizobium meliloti and Penicillium bilaiae

Nodulation of Trifolium repens at low pH by single and paired strains of Rhizobium leguminosarum biovar trifolii

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