Throughout their lifespan, monopile foundations supporting offshore wind turbines inevitably experience horizontal loads from waves, winds, and currents, resulting in cumulative deformation. It has been believed that deformation caused by horizontal loading weakens the interaction between the pile and the soil, leading to a reduction in the ultimate uplift bearing capacity of the pile foundation. However, there is a scarcity of literature investigating this issue, particularly regarding monopiles used in offshore wind turbine installations. Therefore, this study aims to explore the impact of horizontal cyclic loads on the ultimate uplift bearing capacity of monopile, focusing on the pile–soil interaction. To achieve this, a series of 1 g model tests were conducted on a rigid model pile embedded in silt with varying relative compaction. The test results indicate that the ultimate uplift bearing capacity of the pile is significantly diminished after experiencing horizontal cyclic loading, and the extent of reduction is closely linked to the amplitude of the horizontal deformation. A semi-empirical model is developed to predict the ultimate uplift bearing capacity of the pile foundation following horizontal cyclic loading. The key findings of this study are as follows: (1) The earth pressure in the active zone gradually decreases with an increasing number of cycles, while the earth pressure in the passive zone experiences a slight increase under horizontal cyclic loading. (2) The position of the pile rotation center under horizontal cyclic loading is approximately 0.84 times the depth at which the pile is buried, and this relationship appears to be independent of soil density and cyclic load ratio. (3) The variation of earth pressure corresponding to the horizontal deformation of the pile in the active zone can be divided into three phases: a rapid decline phase, a slow decline phase, and a stable phase. (4) The reduction in the ultimate uplift capacity is influenced by the cyclic ratio and number of cycles but does not appear to have a significant relationship with soil density.