This study investigated organic acid and volatile fatty acid components of sweet chestnut fruits collected from naturally growing chestnut trees within the borders of Giresun province. For this purpose, chestnut samples were collected from 10 trees in different regions of Giresun province. The organic acid composition of chestnuts was determined by high-performance liquid chromatography (HPLC). Essential oil components were determined by gas chromatography-mass spectrometry (GC-MS). Five different organic acids, including oxalic, quinic, maleic, citric and succinic acid, were investigated in chestnut samples, and the highest oxalic and citric acid concentrations were found in chestnut samples. In the GC-MS analysis of chestnut samples, 18 fatty acids were detected and terpilonene (TPO) was the most abundant among them. Another vital component found in chestnut samples is limonene.