Variation in the Contents of Neochlorogenic Acid, Chlorogenic Acid and Three Quercetin Glycosides in Leaves and Fruits of Rowan (Sorbus) Species and Varieties from Collections in Lithuania

Abstract

Our research aim was to investigate the quantitative composition of neochlorogenic acid, chlorogenic acid, rutin, hyperoside and isoquercitrin in leaves and fruit of introduced rowan plants to Lithuania using a HPLC method. In leaf and fruit samples, quantitative estimation of neochlorogenic acid (in leaf samples 1.11-11.49 mg/g, in fruit samples 0.93-5.43 mg/g), chlorogenic acid (in leaf samples 2.74-21.91 mg/g, in fruit samples 0.55-7.50 mg/g), rutin (in leaf samples 0.03-4.88 mg/g, in fruit samples 0.02-0.39 mg/g), hyperoside (in leaf samples 0.15-7.44 mg/g, in fruit samples 0.02-1.19 mg/g) and isoquercitrin (in leaf samples 0.75-5.24 mg/g, in fruit samples 0.02-0.65 mg/g) was performed. In leaf samples, the total content of identified phenolic compounds ranged from 7.18 to 35.74 mg/g. In fruit, raw material total content of neochlorogenic acid, chlorogenic acid and identified flavonoids varied from 2.24 to 11.19 mg/g. Comparison of raw material samples of Sorbus species according to their quantitative composition of identified phenolic compounds using cluster analysis singularized S. commixta leaf and fruit samples. These contained the highest contents of chlorogenic acid, hyperoside and isoquercitrin, but also the lowest contents of neochlorogenic acid and rutin. The highest contents of neochlorogenic acid were recorded for S. lancifolia leaves, and of rutin for S. anglica leaves.

Assessment of Antioxidants by HPLC-MS in Grapevine Seeds

“…Hyperoside is quercetin-3-O-galactoside. It was found out in strawberries [Bagdonaite et al 2013], blueberries [Hicks et al 2012], whitethorn [Belkhir et al 2013] and rowan [Gaivelyte et al 2013]. Among herbs, it is abundant above all in St John's wort [Cirak et al 2013].…”

Phytochemical Profiling of Fruit Powders of Twenty Sorbus L. Cultivars

“…Separation of flavonols and phenolic acid was performed according to the methodology described by Gaivelyte et al [ 40 , 41 ] using an ACE (ACT, Aberdeen, UK) column (C18, 150 mm × 4.6 mm, particle size 3 μm). The mobile phase of the optimized chromatographic method consisted of eluent A (0.05% trifluoracetic acid) and B (acetonitrile).…”

“…Validation parameters—linearity and range of linearity, limits of detection and limits of quantification, intra-day repeatability and intermediate precisions, were assessed according to the ICH guidelines. The results of validation were described in our previous studies [ 40 , 41 , 42 ].…”

The Sorbus spp.—Underutilised Plants for Foods and Nutraceuticals: Review on Polyphenolic Phytochemicals and Antioxidant Potential

“…Gaivelyte et al analysed leaf and fruit material of 10 Sorbus spp. and 9 cultivars and found that the TPC varied approximately 5 times, both in leaf and fruit samples, i.e., in the range of 7.18–35.74 mg/g and 2.24–11.19 mg/g, respectively [ 56 ]. The berries of S. aria and S. aucuparia grown at different altitudes were compared; however, there was no correlation between TPC, total proanthocyanidins, radical scavenging capacity and growing site.…”