Variation in the photosynthetic activities of the dinoflagellate Akashiwo sanguinea during formation of resting cysts

Liu, Yun; Chen, Tiantian; Wang, Xiaoting; Song, Shuqun; Li, Caiwen

doi:10.1007/s00227-020-03774-y

Cited by 7 publications

(3 citation statements)

References 62 publications

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“…To inhibit the growth of fungus and bacteria, an antibiotic-antimycotic solution, with final concentrations of 0.05 μg ml −1 amphotericin B, 100 μg ml −1 streptomycin, and 100 I. U. penicillin (Solarbio Inc., Beijing, China), was added to the medium prior to inoculation. This antibiotic mixture had no negative effects on the growth and survival of A. sanguinea, as determined in preliminary experiments (Chen et al, 2015;Liu et al, 2020). The stock culture was maintained in the exponential growth phase by transferring into fresh f/2-Si medium bi-weekly.…”

Section: Algal Isolation and Maintenancementioning

confidence: 91%

“…Furthermore, more efforts have been made to explore the eco-physiology of A. sanguinea at the molecular level. For instance, Deng et al (2015) and Liu et al (2020) characterized the Hsp 70 gene and the photosynthesis-related genes in A. sanguinea during the formation of resting cysts, respectively. The abundance of transcriptome data can facilitate the investigation of biochemical and physiological processes, while only hundreds of Expressed Sequence Tag (EST) sequences and several second-generation transcriptome sequencing data are obtained in NCBI database for A. sanguinea , and no full-length transcriptome is openly available, which limited the basic genetic and functional studies in A. sanguinea .…”

Section: Introductionmentioning

confidence: 99%

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Full-length transcriptome analysis of the bloom-forming dinoflagellate Akashiwo sanguinea by single-molecule real-time sequencing

et al. 2022

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The dinoflagellate Akashiwo sanguinea is a harmful algal species and commonly observed in estuarine and coastal waters around the world. Harmful algal blooms (HABs) caused by this species lead to serious environmental impacts in the coastal waters of China since 1998 followed by huge economic losses. However, the full-length transcriptome information of A. sanguinea is still not fully explored, which hampers basic genetic and functional studies. Herein, single-molecule real-time (SMRT) sequencing technology was performed to characterize the full-length transcript in A. sanguinea. Totally, 83.03 Gb SMRT sequencing clean reads were generated, 983,960 circular consensus sequences (CCS) with average lengths of 3,061 bp were obtained, and 81.71% (804,016) of CCS were full-length non-chimeric reads (FLNC). Furthermore, 26,461 contigs were obtained after being corrected with Illumina library sequencing, with 20,037 (75.72%) successfully annotated in the five public databases. A total of 13,441 long non-coding RNA (lncRNA) transcripts, 3,137 alternative splicing (AS) events, 514 putative transcription factors (TFs) members from 23 TF families, and 4,397 simple sequence repeats (SSRs) were predicted, respectively. Our findings provided a sizable insights into gene sequence characteristics of A. sanguinea, which can be used as a reference sequence resource for A. sanguinea draft genome annotation, and will contribute to further molecular biology research on this harmful bloom algae.

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Section: Algal Isolation and Maintenancementioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Full-length transcriptome analysis of the bloom-forming dinoflagellate Akashiwo sanguinea by single-molecule real-time sequencing

et al. 2022

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“…The transcriptions of photosynthesis-related genes, including psbA, psbD, psaA, psaB, petA, and petB, were detected with the quantitative Real Time PCR (qRT-PCR) assay using the specific primers reported in Liu et al (2020). To normalize the mRNA expression of the target genes, 18S rRNA, TUA and GAPDH were selected as endogenous control and their expression stability in Amoebophrya infected cultures was confirmed prior to the experiments based on the assays described in Pfaffl et al (2004) and Deng et al (2016).…”

Section: Transcript Analysis Of Photosynthesis-related Genes Of Akashiwo Sanguinea During the Course Of Infectionmentioning

confidence: 99%

Chloroplast Ultrastructure and Photosynthetic Response of the Dinoflagellate Akashiwo sanguinea Throughout Infection by Amoebophrya sp.

Chen

Liu

et al. 2021

Front. Mar. Sci.

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The endoparasitic dinoflagellate Amoebophrya infects a number of marine dinoflagellates, including toxic and harmful algal bloom-forming species. The parasite kills its host and has been proposed to be a determining factor in the demise of dinoflagellate blooms in restricted coastal waters. Previous studies have mainly focused on the occurrence, prevalence, and diversity of Amoebophrya, while the interactions between the parasite and its host have received limited attention. Herein, an Amoebophrya sp.-Akashiwo sanguinea co-culture was established from Chinese coastal waters, and morphological, physiological, and transcriptional changes throughout an infection cycle of the parasite were systemically studied. The parasitic dinoflagellate was very infectious, resulting in an infection rate up to 85.83% at a dinospore:host ratio of 10:1. Infected host cells died eventually and released approximately 370 dinospores/cell. The host nuclear structures were rapidly degraded by Amoebophrya infection, and the chloroplasts of parasitized host cells remained intact until the parasite filled the almost entire cell structure. Nevertheless, infected cultures showed sustained but lower levels of photosynthetic performance (∼64% of control cultures), and the photosynthesis-related genes were significantly down-regulated. These findings provide a better understanding of the biological basis of the complex parasite-host interactions, which will be helpful to further elucidate the ecological significance of parasitic dinoflagellates in marine ecosystems.

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Response of benthic dinoflagellates Amphidinium carterae and Bysmatrum gregarium to salinity changes and prolonged darkness: elucidation through laboratory experiments

Rodrigues

Patil

2022

Aquat Ecol

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Variation in the photosynthetic activities of the dinoflagellate Akashiwo sanguinea during formation of resting cysts

Cited by 7 publications

References 62 publications

Full-length transcriptome analysis of the bloom-forming dinoflagellate Akashiwo sanguinea by single-molecule real-time sequencing

Full-length transcriptome analysis of the bloom-forming dinoflagellate Akashiwo sanguinea by single-molecule real-time sequencing

Chloroplast Ultrastructure and Photosynthetic Response of the Dinoflagellate Akashiwo sanguinea Throughout Infection by Amoebophrya sp.

Response of benthic dinoflagellates Amphidinium carterae and Bysmatrum gregarium to salinity changes and prolonged darkness: elucidation through laboratory experiments

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