Variation in Volatile Leaf Oils of Twelve Eucalyptus Species Harvested from Hajeb Layoun Arboreta (Tunisia)

Abstract

Hydrodistillation of the dried leaves of twelve species of the genus Eucalyptus L' Hér., i.e., E. brockwayi C. A. Gardn., E. gracilis F. Muell., E. gillii Maiden, E. largiflorens F. Muell., E. loxophleba Benth., E. occidentalis Endl., E. oldfieldii F. Muell., E. salmonophloia F. Muell., E. sargentii Maiden, E. stricklandii Maiden, E. torquata Luehm., and E. woodwardii Maiden, harvested from Hajeb Layoun arboreta (region of Kairouan, central Tunisia) in January 2005, afforded essential oils in yields varying from 0.5+/-0.1 to 5.7+/-0.5%, dependent on the species. E. sargentii and E. brockwayi provided the highest and the lowest percentage of essential oil amongst all the species examined, respectively. Analysis by GC (RI) and GC/MS allowed the identification of 133 components, representing 92.9-98.8% of the total oil. The contents of the different samples varied according to the species. The main components were 1,8-cineole, terpinen-4-ol, alpha-pinene (2), p-cymene, aromadendrene (1), globulol (5), trans-pinocarveol (6), spathulenol (7), beta-eudesmol, torquatone (3), and 4-methylpentan-2-yl acetate (8). The principal component analysis and the hierarchical clustering indicated that the volatile leaf oil composition of the twelve Eucalyptus species could be clearly differentiated.

Impact of geographic’s variation on the essential oil yield and chemical composition of three Eucalyptus species acclimated in Tunisia

“…The oil collected from each plant was dried (Na2SO4) and stored at 4°C until analysis. The yield of essential oil was expressed in % (v/w) of the dry material (Elaissi et al, 2010a;Elaissi et al, 2010b).…”

“…Eucalyptus essential oil was also reported as an effective anti-inflammatory, analgesic, antioxidant, antimutagenic, insecticide, nematocide and acaricide oil (Batish et al, 2008;Bugarin et al, 2014;Rossi & Palacios, 2015).The Eucalyptus essential oils can be obtained by different methods, such as hydrodistillation, supercritical CO2 extraction, microwave-assisted extraction, and vacuum extraction by solvents. These methods affect the final yield of the oil and it varied from 0.1± 0.1 to 5.7±0.5 7% (Elaissi et al, 2010a;Elaissi et al, 2010b). High levels of 1,8-cineole (87.8%) were found for the majority of the Eucalyptus species (Zrira et al, 2004;Elaissi et al, 2010c).…”

Variation in Volatile Leaf Oils of Eleven Eucalyptus Species Harvested from Korbous Arboreta (Tunisia)

“…They were used essentially as a fire wood, for the production of mine wood, and in the fight against erosion [14]. In our previous investigations on the essential oils of Eucalyptus species grown in Tunisia, we have characterized the leaf essential oil of seven [15] and twelve species [16] from Hajeb Layoun arboreta (region of Kairouan, center of Tunisia). Here, we report the results of our research on the variability of the leaf essential oils of eleven Eucalyptus species collected from Korbous arboreta (region of Nabeul, north east of Tunisia).…”

Uso de semioquímicos en el control de plagas. Estudios básicos y de aplicación

“…Los datos obtenidos se sometieron a un análisis de componentes principales (PCA), una metodología poco convencional en la búsqueda de volátiles de insectos y sin precedentes en el campo de las moscas de la fruta. Este método estadístico multivariante es muy común en otros campos, como el análisis alimentario (Jabalpurwala et al, 2009;Elaissi et al, 2010;Giri et al, 2010;Mildner-Szkudlarz y Jelen, 2010;Zhang et al, 2010), pero por lo que respecta a los insectos, solo se ha aplicado a especies de himenópteros (Steiner et al, 2007;Coppee et al, 2008). La técnica de PCA permite realizar agrupaciones de los compuestos identificados de acuerdo con los factores estudiados.…”

“…This multivariate method has been extensively employed for studies on food volatiles (Jabalpurwala et al, 2009;Elaissi et al, 2010;Giri et al, 2010;Mildner-Szkudlarz & Jelen;Zhang et al, 2010). But regarding to insects, PCA has been employed only to study the volatiles of Hymenopteran species (Steiner et al, 2007;Coppee et al, 2008).…”