The new coronavirus infection (COVID-19) that appeared suddenly has permeated our daily lives and changed our way of life. In the field of education, education is being conducted in a non-face-to-face teaching method to prevent the spread of coronavirus. In the end, e-Learning, a new educational and training system that can provide a lifelong education environment in the 21st century information society, is increasing in use in the field of education. The biggest advantage of the online education system is that it provides an environment in which you can learn the necessary contents anytime, anywhere. However, there are cases in which the learning effect is reduced because various learning support is not available in the online space due to the sudden change of the educational environment due to the covid-19. Therefore, in this thesis, a study was conducted to analyze the learning effect of online education with voice signals for college students who are receiving education through an online education environment. To this end, the learning effect was classified into a group saying that the learning effect increased and a group that decreased due to online education, and the voices of the subjects in each group were collected. As a result of the experiment, the results of the vocal cord vibration (Pitch), Degree of voice breaks, Jitter and Shimmer were consistent among the elements of voice signal analysis between two groups.