Variation of 4 MV X-ray dose rate in fractionated irradiation strongly impacts biological endothelial cell response in vitro

Abstract

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Tissue Reactions and Mechanism in Cardiovascular Diseases Induced by Radiation

“…Human umbilical vein endothelial cells (HUVECs) have been mostly used to study the effects and mechanisms of radiation on blood vessels at the cellular level in vitro. In addition to the canonical nuclear genomic DNA damage response and proliferation inhibition [ 20 , 25 , 26 ], other various forms of radiation effects on HUVECs were observed, which include senescence-associated secretory phenotype (SASP) and premature senescence [ 27 , 28 , 29 ], increased intrinsic ROS and production and release of pro-inflammatory cytokines [ 30 , 31 , 32 , 33 ], decreasing mitochondrial membrane potential [ 31 ], suppression of the autophagic flux [ 34 ], endothelial-to-mesenchymal transition (EndMT) [ 35 ], decreasing the capacity of forming capillary-like network [ 36 , 37 ], and increased permeability and disruption of cellular junctions [ 33 ]. The types of cell death of HUVECs induced by radiation include apoptosis [ 38 ], pyroptosis [ 39 ], and ferroptosis [ 40 ].…”

Comparing cellular response to two radiation treatments based on key features visualization

“…Efforts have been made to understand the molecular response of these cells at different molecular scales by multi-omics approaches, generating a wealth of valuable data to go further in the use of endothelial cells as a predictive tool (Heinonen et al, 2015; Jaillet et al, 2017; Heinonen et al, 2018; Benadjaoud et al, 2022; Morilla et al, 2022). In this sense, we have established proofs of concept that multiparametric measurements on these cells make it possible to highlight differences in response between irradiation modalities differing by the energy or the dose rate of the radiation (Paget et al, 2019; Ben Kacem et al, 2020, 2022).…”