Variation of Astragalosides Contents in Cultivated Astragalus membranaceus

Kim, Seung Hyun; Jun, Yoon Mi; Lim, Ju Jin; Kim, Sung Hyop; Chung, Ill Min; Kim, Eun Hye

doi:10.7783/kjmcs.2012.20.5.372

Cited by 9 publications

(5 citation statements)

References 16 publications

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“…To create a standard curve, quercetin and gallic acid were used as reference materials. The amount of total phenol was calculated in accordance with Lim et al [ 35 ]. In summary, the sample (200 μL), 1 mL of FolinDenis, and 0.8 mL of sodium carbonate were combined and left to incubate for 45 min in the dark.…”

Section: Methodsmentioning

confidence: 99%

Effect of hot-melt extruded Morus alba leaves on intestinal microflora and epithelial cells

Kim,

Go,

Baek

2024

Heliyon

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Section: Methodsmentioning

confidence: 99%

Effect of hot-melt extruded Morus alba leaves on intestinal microflora and epithelial cells

Kim,

Go,

Baek

2024

Heliyon

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“…Analysis of differences in cell size of leaf and cell thickness of stem using SEM image of A. membranaceus sprout under three types of LED light sources. (1) White Red Blue Size of leaf cell (μm) 20.67 ± 6.50 c 58.57 ± 6.17 a 36. Table 1.…”

Section: Comparison Of the Cellular Morphologies Of A Membranaceus Sp...mentioning

confidence: 99%

“…Astragalus membranaceus Bunge, commonly referred to as Mongolian milkvetch, is an herbaceous perennial plant belonging to the Leguminosae family. In Republic of Korea, Plants 2023, 12,1914 2 of 22 high-quality A. membranaceus is grown in Jeongseon, Gangwon-do, and has been used as a medicinal plant since ancient times [1,2]. A. membranaceus is naturally widely distributed in Asian regions such as Korea and China and in parts of Europe and Africa [3].…”

Section: Introductionmentioning

confidence: 99%

Cellular Morphology and Transcriptome Comparative Analysis of Astragalus membranaceus Bunge Sprouts Cultured In Vitro under Different LED Light

Seo

Lee²,

Yoo

et al. 2023

Plants

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Astragalus membranaceus, the major components of which are saponins, flavonoids, and polysaccharides, has been established to have excellent pharmacological activity. After ginseng, it is the second most used medicinal plant. To examine the utility of A. membranaceus as a sprout crop for plant factory cultivation, we sought to establish a functional substance control model by comparing the transcriptomes of sprouts grown in sterile, in vitro culture using LED light sources. Having sown the seeds of A. membranaceus, these were exposed to white LED light (continuous spectrum), red LED light (632 nm, 1.58 μmol/m2/s), or blue LED light (465 nm, 1.44 μmol/m2/s) and grown for 6 weeks; after which, the samples were collected for transcriptome analysis. Scanning electron microscopy analysis of cell morphology in plants exposed to the three light sources revealed that leaf cell size was largest in those plants exposed to red light, where the thickest stem was observed in plants exposed to white light. The total number of genes in A. membranaceus spouts determined via de novo assembly was 45,667. Analysis of differentially expressed genes revealed that for the comparisons of blue LED vs. red LED, blue LED vs. white LED, and red LED vs. white LED, the numbers of upregulated genes were 132, 148, and 144, respectively. Binding, DNA integration, transport, phosphorylation, DNA biosynthetic process, membrane, and plant-type secondary cell wall biogenesis were the most enriched in the comparative analysis of blue LED vs. red LED, whereas Binding, RNA-templated DNA biosynthetic process, DNA metabolic process, and DNA integration were the most enriched in the comparative analysis of blue vs. white LED, and DNA integration and resolution of meiotic recombination intermediates were the most enrichment in the comparison between red LED vs. white LED. The GO term associated with flavonoid biosynthesis, implying the functionality of A. membranaceus, was the flavonoid biosynthetic process, which was enriched in the white LED vs. red LED comparison. The findings of this study thus indicate that different LED light sources can differentially influence the transcriptome expression pattern of A. membranaceus sprouts, which can provide a basis for establishing a flavonoid biosynthesis regulation model and thus, the cultivation of high-functional Astragalus sprouts.

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“…특히 황기의 flavonoid 성분은 다양한 생리활성의 주요 활성물 질로 평가되고 있다 (Kim and Kim, 1997;Kim et al, 2002). 황기의 주요 triterpenoid glycosides로는 astragalosides 화합물 이 알려져 있으며, 연근별, 수확시기별 astragalosides 함량변이 가 보고되기도 하였다 (Kim et al, 2012). …”

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Evaluation on Extraction Conditions and HPLC Analysis Method for Bioactive Compounds of Astragali Radix

Kim¹,

Lee²,

Lee³

et al. 2013

Korean Journal of Medicinal Crop Science

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: This study has been conducted to establish the optimal extraction process and HPLC analysis method for the determination of marker compounds as a part of the materials standardization for the development of health functional food materials from Astragali radix. Five extraction conditions including the shaking extraction at room temperature and the reflux extraction at 85℃ with 30%, 50% and 95% ethanol were evaluated. Reflux extraction with 50% ethanol showed the highest extraction yield as 27.27 ± 2.27%, while the extraction under reflux with 95% ethanol showed significantly the lowest yield of 10.55 ± 0.24%. The quantitative determination methods of calycosin-7-O-β-D-glucoside and calycosin as marker compounds of Astragali radix extracts were optimized by HPLC analysis using a Thermo Hypersil column (4.6 × 250 ㎜, 5 ㎛) with the gradient elution of water and acetonitrile as the mobile phase at the flow rate of 0.8 mL min -1 and a detection wavelength of 230 ㎚. The HPLC/UV method was applied successfully to the quantification of two marker compounds in Astragali radix extracts after validation of the method with the linearity, accuracy and precision. The contents of calycosin-7-O-β-D-glucoside and calycosin in 50% ethanol extracts by reflux extraction were significantly higher as 1,700.3 ± 30.4 and 443.6 ± 8.4 ㎍ g-1, respectively, comparing with those in other extracts. The results indicate that the reflux extraction with 50% ethanol at 85℃ is optimal for the extraction of Astragali radix, and the established HPLC method are very useful for the evaluation of marker compounds in Astragali radix extracts to develop the health functional material from Astragali radix.

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Variation of Astragalosides Contents in Cultivated Astragalus membranaceus

Cited by 9 publications

References 16 publications

Effect of hot-melt extruded Morus alba leaves on intestinal microflora and epithelial cells

Effect of hot-melt extruded Morus alba leaves on intestinal microflora and epithelial cells

Cellular Morphology and Transcriptome Comparative Analysis of Astragalus membranaceus Bunge Sprouts Cultured In Vitro under Different LED Light

Evaluation on Extraction Conditions and HPLC Analysis Method for Bioactive Compounds of Astragali Radix

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