Variation of Microcystin Content of Cyanobacterial Blooms and Isolated Strains in Lake Grand-Lieu (France)

Vezie, C.; Brient, Luc; Sivonen, Kaarina; Bertru, G.; Lefeuvre, Jean‐Claude; Salkinoja‐Salonen, Mirja

doi:10.1007/s002489900067

Cited by 153 publications

(92 citation statements)

References 34 publications

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“…These values are similar to those reported from other countries: 72% in Germany (Fastner et al, 1999), 44-56% in Scandinavia (Berg et al, 1986;Sivonen et al, 1990), 90% in Holland (Leeuwangh et al, 1983), 66% in Denmark (Henriksen & Moestrup, 1997) and 74% in Canada (Kotak et al, 1995). In Mediterranean countries, 70% in France (Vezie et al 1997), 60% in Portugal (Vasconcelos, 1994), and all analyzed blooms in Greece (Cook et al 2004) were toxic. The frequency of toxic blooms in Europe indicates that this is, at least, a pan-European problem, that should be regarded as a priority in relation to the water quality of surface water bodies used for drinking and recreation.…”

Section: Discussionmentioning

confidence: 99%

Cyanobacterial abundance and microcystin occurrence in Mediterranean water reservoirs in Central Spain: microcystins in the Madrid area

Carrasco

Moreno

Sanchis

et al. 2006

European Journal of Phycology

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The occurrence and concentration of microcystins were analysed in sestonic samples from seven water reservoirs in the Madrid region (Spain) between July and November in two consecutive years (2002 and 2003). The data collected indicate that microcystins were present on several occasions in both years in all the reservoirs studied. The months of maximum risk for cyanotoxin occurrence were July, September and October, when Microcystis typically dominated the phytoplankton community. Four of the seven reservoirs exhibited conspicuous blooms, three of which were toxic. Of the samples in 2003, 45% and 70%, respectively, contained microcystins (Mc). In four of the reservoirs, microcystin concentrations were higher than the WHO recommended limit for drinking water (1 mg Mc l . From the data obtained we can conclude that M. aeruginosa is the main producer of microcystins in freshwaters from the Madrid region.

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Section: Discussionmentioning

confidence: 99%

Cyanobacterial abundance and microcystin occurrence in Mediterranean water reservoirs in Central Spain: microcystins in the Madrid area

Carrasco

Moreno

Sanchis

et al. 2006

European Journal of Phycology

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“…This discussion assumes, that A. circinalis did not produce any MCs. Although 2 out of 24 French strains of A. circinalis were reported to synthesize MCs (Vezie et al, 1998), no MCs production was found in Australian strains (Velzeboer et al, 2000). In the case of M. aeruginosa there are two possible explanations for this phenomenon of varying MCconcentrations: (1) the species can switch toxin production on and off, depending on the situation given in the environment; (2) there are two or more strains of M. aeruginosa, 'toxin producers' and 'non-producers' present at the same time.…”

Section: Discussionmentioning

confidence: 99%

Occurrence and elimination of cyanobacterial toxins in two Australian drinking water treatment plants

Hoeger

Shaw²,

Hitzfeld

et al. 2004

Toxicon

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In Australian freshwaters, Anabaena circinalis, Microcystis spp. and Cylindrospermopsis raciborskii are the dominant toxic cyanobacteria. Many of these surface waters are used as drinking water resources. Therefore, the National Health and Medical Research Council of Australia set a guideline for MC-LR toxicity equivalents of 1.3 mg/l drinking water. However, due to lack of adequate data, no guideline values for paralytic shellfish poisons (PSPs) (e.g. saxitoxins) or cylindrospermopsin (CYN) have been set. In this spot check, the concentration of microcystins (MCs), PSPs and CYN were determined by ADDA-ELISA, cPPA, HPLC-DAD and/or HPLC-MS/MS, respectively, in two water treatment plants in Queensland/Australia and compared to phytoplankton data collected by Queensland Health, Brisbane. Depending on the predominant cyanobacterial species in a bloom, concentrations of up to 8.0, 17.0 and 1.3 mg/l were found for MCs, PSPs and CYN, respectively. However, only traces (,1.0 mg/l) of these toxins were detected in final water (final product of the drinking water treatment plant) and tap water (household sample). Despite the low concentrations of toxins detected in drinking water, a further reduction of cyanobacterial toxins is recommended to guarantee public safety. q

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“…Techniques that are based on highly repetitive sequences have been used for identification of toxic, planktic Anabaena, Nostoc (Rouhiainen et al, 1995) and Cylindrospermopsis (Wilson et al, 2000) strains. Furthermore, fingerprinting of the repetitive extragenic palindromic (REP) elements and\or enterobacterial repetitive intergenic consensus (ERIC) sequences has been used for identification of symbiotic (Rasmussen & Svenning, 1998) and free-living cyanobacteria (Rasmussen & Svenning, 1998 ; Lehtima$ ki et al, 2000).We have isolated planktic anatoxin-a (Sivonen et al, 1989), microcystin-producing (Luukkainen et al, 1993, 1994 Sivonen et al, 1992Sivonen et al, , 1990Sivonen et al, , 1995Vezie et al, 1998) and non-toxic cyanobacterial strains from fresh (Finnish and French) and brackish waters and purified them axenically (Rouhiainen et al, 1995). In this study, we characterized microcystin-producing Anabaena, Nostoc, Planktothrix (Oscillatoria agardhii) and Microcystis strains, that belong to six known microcystin producing genera (Sivonen & Jones, 1999), by RFLP and sequencing of the 16S rRNA gene, and by REP-and ERIC-PCR.…”

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Molecular characterization of planktic cyanobacteria of Anabaena, Aphanizomenon, Microcystis and Planktothrix genera.

Lyra¹,

Suomalainen²,

Gugger³

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

218

159

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Toxic and non-toxic cyanobacterial strains from Anabaena, Aphanizomenon, Calothrix, Cylindrospermum, Nostoc, Microcystis, Planktothrix (Oscillatoria agardhii), Oscillatoria and Synechococcus genera were examined by RFLP of PCR-amplified 16S rRNA genes and 16S rRNA gene sequencing. With both methods, high 16S rRNA gene similarity was found among planktic, anatoxin-aproducing Anabaena and non-toxic Aphanizomenon, microcystin-producing and non-toxic Microcystis, and microcystin-producing and non-toxic Planktothrix strains of different geographical origins. The respective sequence similarities were 999-100 %, 942-999 % and 993-100 %. Thus the morphological characteristics (e.g. Anabaena and Aphanizomenon), the physiological (toxicity) characteristics or the geographical origins did not reflect the level of 16S rRNA gene relatedness of the closely related strains studied. In addition, cyanobacterial strains were fingerprinted with repetitive extragenic palindromic ( Abbreviations : ERIC, enterobacterial repetitive intergenic consensus ; LTRR, long tandemly repeated sequence ; ML, maximum-likelihood ; MP, maximum-parsimony ; NJ, neighbour-joining ; REP, repetitive extragenic palindromic ; STRR, short tandemly repeated sequence ; UPGMA, unweighted pairs group method with averages.The GenBank/EMBL accession numbers for the cyanobacterial 16S rRNA gene sequences are AJ133151-AJ133154, AJ133156, AJ133157, AJ133159-AJ133170, AJ133172-AJ133176 and AJ133185 bacteria occur in a wide range of habitats. In eutrophic fresh and brackish waters cyanobacteria form toxic water blooms which have caused human and animal poisonings (Ressom et al., 1994 ; Kuiper-Goodman et al., 1999). The most frequently found toxins in cyanobacterial blooms worldwide are hepatotoxic cyclic peptides, microcystins and nodularins (Sivonen & Jones, 1999). Mass occurrences of cyanobacteria that contain neurotoxins [anatoxin-a, anatoxin-a(S) and saxitoxins] have been found in Australia, Europe and North America (Sivonen & Jones, 1999). Cyanobacteria are a morphologically diverse group of organisms ranging from unicellular to filamentous forms. Traditionally, the classification of cyanobacteria has been based on morphological characters, Asayama et al. (1996) ; 2, Giovannoni et al. (1988) ; 3, Herdman et al. (1979a) ; 4, Herdman et al. (1979b) ; 5, Kenyon et al. (1972) ; 6, Kondo et al. (2000) ; 7, Lachance (1981) ; 8, Leeuwangh et al. (1983) ; 9, Lehtima$ ki et al. (2000) ; 10, Lu et al. (1997) ; 11, Luukkainen et al. (1993) ; 12, Luukkainen et al. (1994) ; 13, Lyra et al. (1997) ; 14, Masephol et al. (1996) ; 15, Mazel et al. (1990) ; 16, Neilan et al. (1995) ; 17, Neilan et al. (1997a) ; 18, Neilan et al. (1997b) ; 19, Neilan et al. (1999) ; 20, Otsuka et al. (1999) ; 21, Rapala et al. (1993) ; 22, Rasmussen & Svenning (1998) ; 23, Rippka & Herdman (1992) ; 24, Rippka et al. (1979) ; 25, Rouhiainen et al. (1995) ; 26, Rudi & Jakobsen (1999) ; 27, Rudi et al. (1997) ; 28, Rudi et al., 1998 ; 29, Sivonen et al. (1989) ; 30, Sivonen et al. (1990) ;...

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Variation of Microcystin Content of Cyanobacterial Blooms and Isolated Strains in Lake Grand-Lieu (France)

Cited by 153 publications

References 34 publications

Cyanobacterial abundance and microcystin occurrence in Mediterranean water reservoirs in Central Spain: microcystins in the Madrid area

Cyanobacterial abundance and microcystin occurrence in Mediterranean water reservoirs in Central Spain: microcystins in the Madrid area

Occurrence and elimination of cyanobacterial toxins in two Australian drinking water treatment plants

Molecular characterization of planktic cyanobacteria of Anabaena, Aphanizomenon, Microcystis and Planktothrix genera.

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