Variation of micronucleated erythrocytes in peripheral blood of <i>Sciurus aureogaster</i> in relation to age: An increment of micronucleated polychromatic erythrocytes after the administration of colchicine

Zúñiga‐González, Guillermo Moisés; Torres-Bugarı́n, Olivia; Ramos-Ibarra, María Luisa; Zamora‐Pérez, Ana Lourdes; Gómez‐Meda, Belinda Claudia; Ventura-Aguilar, A.J.; Ramos-Mora, Alberto; Ortíz, Genaro Gabriel; Alvarez-Moya, Carlos; González-Rodríguez, Andrés; Luna-Aguirre, Jaime; Gallegos‐Arreola, Martha Patricia

doi:10.1002/em.1025

Cited by 24 publications

(20 citation statements)

References 5 publications

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“…The use of non-human organisms to study genotoxic agents is rapidly increasing [Ruiz et al, 1992;Al-Sabti and Metcalfe, 1995;Burgeot et al, 1995;Choy et al, 1995;Jena and Bhunya, 1995;Ma et al, 1995;Zúñiga-González et al, 1998, 2001a, 2001b, and amphibians are of special interest in this regard due to their ecological niche [Jaylet et al, 1986;Krauter et al, 1987;Le et al, 1992;Rudek and Rozek, 1992;Fernandez and I'Haridon, 1994]. Urodela, which includes salamanders and newts, is one of the three surviving orders of the amphibian class.…”

Section: Introductionmentioning

confidence: 99%

Induction of micronucleated cells in the shed skin of salamanders (Ambystoma sp.) treated with colchicine or cyclophosphamide

Zamora‐Pérez

Zúñiga‐González

Gómez‐Meda

et al. 2004

Environ and Mol Mutagen

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The micronucleus (MN) assay can be used to detect the genotoxic effects of chemical agents in virtually any cell that divides frequently. Salamanders (Ambystoma sp.) are amphibians that can be easily maintained and bred in the laboratory and spontaneously shed their skin every 2.5-4 days. In this present study, we have evaluated the usefulness of this shed skin for the MN assay. We exposed salamanders to different concentrations of both the aneugen colchicine (COL) and the clastogen cyclophosphamide (CP) and we determined the frequency of micronucleated cells (MNCs) in their sheds. Fragments of shed skin were placed on clean slides, fixed, stained, observed with a light microscope, and the number of MNCs was counted. The MNC frequency was increased significantly by all doses of COL and CP tested, administered either as single or repeated exposures. The presence of MNCs in the shed skin and the speed of sloughing lead us to propose that the sheds of Ambystoma sp., or other amphibians that slough their skin, are suitable alternative models for detecting genotoxic exposures relevant to aquatic environments.

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Section: Introductionmentioning

confidence: 99%

Induction of micronucleated cells in the shed skin of salamanders (Ambystoma sp.) treated with colchicine or cyclophosphamide

Zamora‐Pérez

Zúñiga‐González

Gómez‐Meda

et al. 2004

Environ and Mol Mutagen

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“…Since 1996, our group has worked on identifying animal species with measurable frequencies of spontaneous micronucleated erythrocytes (MNEs) circulating in their peripheral blood [Zúñiga et al, 1996a;Zúñiga-González et al, 2000;Zúñiga-González et al, 2001a;Zúñiga-González et al, 2002], with the idea that analysis of MNE frequencies in these animals can be used as a biomonitor of exposure to genotoxic agents [Zúñiga-González et al, 1998;Zúñiga-González et al, 2001b;Gómez-Meda et al, 2004]. A highly proliferating tissue like blood is required to carry out this assay [Lähdetie, 1983;Shuilin and Baker, 1989;Schmezer et al, 1990;Torres-Bugarín et al, 1998;Zamora-Perez et al, 2004], and only a drop of blood is needed to measure micronucleus (MN) frequencies in peripheral blood, so that it is not necessary to kill the animal [Zúñiga-González et al, 1998;Zúñiga-González et al, 2001b].…”

Section: Introductionmentioning

confidence: 99%

Micronucleated erythrocyte frequencies in old and new world primates: Measurement of micronucleated erythrocyte frequencies in peripheral blood ofCallithrix jacchus as a model for evaluating genotoxicity in primates

Zúñiga‐González

Gómez‐Meda

Zamora‐Pérez

et al. 2005

Environ. Mol. Mutagen.

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Nonhuman primates are of particular relevance in evaluating the potential toxicity of drugs and environmental agents. We have used previously published information and data from the present study to establish a relationship for New World (NW) and Old World (OW) primates on the basis of the frequency of spontaneous micronucleated erythrocytes (MNEs) observed in peripheral blood. Data on spontaneous MNEs in peripheral blood from 15 species of primates, including humans, indicate that NW primates have significantly (P < 0.01) higher MNE frequencies (group mean, 9.5 +/- 7.3 MNEs/10,000 erythrocytes; range, 0.7-20.5/10,000 erythrocytes) than OW primates (group mean, 1.0 +/- 0.9 MNEs/10,000 erythrocytes; range, 0.0-2.6 MNEs/10,000 erythrocytes). Humans are believed to have developed in the OW, and human MNE frequencies were similar to those described for OW primate species. We selected the common marmoset (Callithrix jacchus), a NW primate, to determine whether therapeutic pediatric doses of Metotrexate (MTX; 2.5 mg/kg), Cyclophosphamide (CP; 5 mg/kg), Cytosine-arabinoside (Ara-C; 3 mg/kg), or 5-Fluorouracil (5-FU; 10 mg/kg), administered daily for two consecutive days, increase the frequency of micronuclei. Micronucleated polychromatic erythrocyte frequencies were increased significantly in groups receiving MTX, CP and Ara-C, while MNE frequencies were increased by the Ara-C treatment. The results of this study indicate that NW primates have higher spontaneous MNE frequencies than OW primates, and because of this, NW primates like the common marmoset, may be suitable for evaluating the genotoxicity of chemical agents.

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“…Since the animals were not obtained from a well-controlled animal facility, there are likely to have been differences in the sex and age of the birds. Even though the birds were of a similar weight, they were not necessarily of similar age, and it has been observed that age can influence the capacity of animals to remove MNE from circulation (Zúñ iga-González et al , 2001a, 2001b.…”

Section: Resultsmentioning

confidence: 99%

“…We previously determined the frequency with which spontaneous micronucleated erythrocytes (MNE) appear in the species Aratinga canicularis (Zú ñiga- González et al , 2000). Interestingly, we observed a high frequency of BE (unpublished data), and, as a result, it is important to determine whether the presence of these abnormal erythrocytes may be inherent to this species or whether a genotoxic agent could have induced the appearance of such structures.…”

Section: Introductionmentioning

confidence: 99%

Nuclear abnormalities in erythrocytes of parrots (Aratinga canicularis) related to genotoxic damage

et al. 2006

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Nuclear abnormalities in erythrocytes, as micronuclei and nuclear buds (BE), are considered potential biomarkers of genotoxic exposure. We described previously the frequency of spontaneous micronucleated erythrocytes (MNE) in the species Aratinga canicularis. Here, we have used this species to evaluate the induction of MNE and BE by mitomycin-C. Animals were given a single intracoelomic injection of 0, 2, 3 or 4 mg/kg mitomycin-C on two consecutive days. A drop of blood was obtained after 0, 24, 48 and 72 h, and stained smears were used to count micronucleated polychromatic erythrocytes (MNPCE) and polychromatic erythrocytes with buds (BPCE)/1000 polychromatic erythrocytes. The number of MNE and BE in 10 000 total erythrocytes was also counted. MNPCE and BPCE frequencies were elevated at 24, 48, and 72 h after the administration of the lower dose (P B/0.03). At a 3 mg/kg dose, the frequency of MNPCE increased at 48 and 72 h (P B/0.04) whereas the number of BPCE increased, but not significantly. Administration of 4 mg/kg mitomycin-C increased the number of MNE observed at 72 h (P B/0.03), the number of MNPCE at 48 h (P B/0.01) and 72 h (P B/0.006), the BE frequency at 72 h (P B/0.05), and the frequency of BPCE at 48 and 72 h (P B/0.001). While mitomycin-C appears to produce a parallel increase in MNPCE and BPCE frequencies, the MNE seemed to be a more sensitive indicator of genotoxicity than the BE. This suggests that evaluating BE and MNE in routine haematological analysis should be considered to evaluate environmental genotoxic exposure.

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Variation of micronucleated erythrocytes in peripheral blood of Sciurus aureogaster in relation to age: An increment of micronucleated polychromatic erythrocytes after the administration of colchicine

Cited by 24 publications

References 5 publications

Induction of micronucleated cells in the shed skin of salamanders (Ambystoma sp.) treated with colchicine or cyclophosphamide

Induction of micronucleated cells in the shed skin of salamanders (Ambystoma sp.) treated with colchicine or cyclophosphamide

Micronucleated erythrocyte frequencies in old and new world primates: Measurement of micronucleated erythrocyte frequencies in peripheral blood ofCallithrix jacchus as a model for evaluating genotoxicity in primates

Nuclear abnormalities in erythrocytes of parrots (Aratinga canicularis) related to genotoxic damage

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