Variation of the Oxidative Profile in Pregnant Women With and Without Gestational Complications

Lucca, Leidiane de; Jantsch, Letícia Bigolin; Vendrame, Silmara Ana; Paula, Hellen Lopes de; Stein, Carolina dos Santos; Gallarreta, Francisco Maximiliano Pancich; Moresco, Rafael Noal; Gonçalves, Thissiane de Lima

doi:10.1007/s10995-022-03475-6

Cited by 11 publications

(14 citation statements)

References 27 publications

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“…In addition, our results could have a prospective value related to the prevention of the development of cardiovascular disease, which has been reported for AMA pregnant women and their children [28,29]. About this issue, a key role as a pathogenic mechanism could be played by oxidative stress and endothelial dysfunction [12,[30][31][32][33]. The data we obtained about plasma levels of NO would confirm this hypothesis.…”

Section: Discussionsupporting

confidence: 79%

“…One of the possible explanations of these phenomena could be the increased oxidative stress. About this issue, it should be pointed out that in physiological pregnancy the increase of oxidants is counterbalanced by the production of antioxidants and the occurrence of oxidative stress is limited [22,23]. Instead, in all conditions in which the systems for regulating the balance between oxidants and antioxidants are poorly effective, the development of oxidative stress is markedly greater.…”

Section: Discussionmentioning

confidence: 99%

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Plasma Redox Balance in Advanced Maternal Age Pregnant Women and Effects of Plasma on Umbilical Cord Mesenchymal Stem Cells

Grossini,

Aquino,

Venkatesan

et al. 2024

Preprint

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Pregnancy at advanced maternal age (AMA) is a condition of potential risk for the development of maternal-fetal complications with possible repercussions even in the long-term. Here, we analyzed the changes of plasma redox balance and the effects of plasma on human umbilical cord mesenchymal cells (hUMSCs) in AMA pregnant women (patients) at various timing of pregnancy. One hundred patients and twenty pregnant women younger than 40 years (controls) were recruited, and evaluated at various timings during pregnancy until after delivery. Plasma samples were used to measure the thiobarbituric acid reactive substances (TBARS), glutathione and nitric oxide (NO). In addition, plasma was used to stimulate the hUMSCs, which were tested for cell viability, reactive oxygen species (ROS) and NO release. The obtained results showed that throughout pregnancy until after delivery in patients the levels of plasma glutathione and NO were lower than those of controls, while those of TBARS were higher. Moreover, plasma of patients reduced cell viability and NO release, and increased ROS release in hUMSCs. Our results highlighted alterations in the redox balance and the presence of potentially harmful circulating factors in plasma of patients They could have clinical relevance for the prevention of complications related to AMA pregnancy.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Plasma Redox Balance in Advanced Maternal Age Pregnant Women and Effects of Plasma on Umbilical Cord Mesenchymal Stem Cells

Grossini,

Aquino,

Venkatesan

et al. 2024

Preprint

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show abstract

“…H 2 O 2 has been used to induce OS both in vivo and in vitro using different cellular systems (32,34). This study suitably used the plasma of the pregnant women just before the CS, a biological source for oxidation/antioxidation mechanisms, by monitoring the MDA level in vitro without the effects of the whole-body biochemical mechanisms on this OS biomarker (6).…”

Section: Discussionmentioning

confidence: 99%

“…Normal pregnancy is associated with various physiologic and hemodynamic changes, especially during the last month very close to the delivery (1, 2), which might affect the response to drugs or anesthetics (3,4). Decreased plasma cholinesterase (ChE) activity and increased oxidative stress (OS) biomarkers in the plasma malondialdehyde (MDA) have been reported in the last trimester of pregnancy (5)(6)(7). The decrease in plasma ChE activity might predispose pregnant women to the possibility of adverse drug reactions when neuromuscular blocking agents or anesthetics are used, especially in cesarean section (CS) delivery (8)(9)(10).…”

Section: Introductionmentioning

confidence: 99%

“…The in vitro assessment of plasma ChE activity and OS biomarkers have been used to minimize invasive activities in patients or experimental animals (22)(23)(24)(25)(26). Furthermore, the in vitro experimental paradigms avoid the possibility of interference via maternal conditions involving anesthetics and surgical manipulations for CS delivery and the expected post-delivery biochemical changes (2,6,14).…”

Section: Introductionmentioning

confidence: 99%

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In vitro Effects of Propofol and Bupivacaine on Pregnant Women’s Plasma Cholinesterase Activity and Malondialdehyde Level

Karam,

Mohammad

2024

J Cell Mol Anesth

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Background: Propofol and bupivacaine are commonly used anesthetics for cesarean section (CS), and they might modulate plasma cholinesterase (ChE) activity and oxidative stress during the last stage of pregnancy. This study aimed to assess the in vitro effects of propofol and bupivacaine on plasma ChE activity and malondialdehyde (MDA) levels in pregnant women before undergoing elective CS. Methods: The plasma samples of 20 women set for elective CS were pooled for the in vitro determination of the effects of propofol and bupivacaine separately on plasma ChE activity (10 minutes of incubation with different concentrations at 37ºC) and the MDA level after the in vitro exposure of plasma samples containing different anesthetic concentrations to H2O2 (100 µM, incubated for 1 hour at 37ºC). Results: Bupivacaine at 1.1 and 2.2 µM significantly inhibited plasma ChE in vitro in a concentration-dependent manner by 13% and 20%, respectively. Propofol at 25 and 50 µM did not affect plasma ChE. A unique finding in this study was that both propofol and bupivacaine revealed an antioxidant effect, as both propofol at concentrations of 25, 50, and 100 µM and bupivacaine at 1.1, 2.2, and 4.4 µM reduced the MDA level in a concentration-dependent manner in vitro after the incubation of plasma samples with H2O2 as a source of oxidant. Conclusions: The in vitro findings suggest that bupivacaine exerts anti-ChE activity that should be taken into consideration in CS anesthesia, and both propofol and bupivacaine possess antioxidant properties that need additional clinical studies.

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Growth restriction in preeclampsia: lessons from animal models

MALLETTE

Crudup

Alexander

2023

Current Opinion in Physiology

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Variation of the Oxidative Profile in Pregnant Women With and Without Gestational Complications

Cited by 11 publications

References 27 publications

Plasma Redox Balance in Advanced Maternal Age Pregnant Women and Effects of Plasma on Umbilical Cord Mesenchymal Stem Cells

Plasma Redox Balance in Advanced Maternal Age Pregnant Women and Effects of Plasma on Umbilical Cord Mesenchymal Stem Cells

In vitro Effects of Propofol and Bupivacaine on Pregnant Women’s Plasma Cholinesterase Activity and Malondialdehyde Level

Growth restriction in preeclampsia: lessons from animal models

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