Variations among clinical isolates of Staphylococcus aureus to induce expression of E-selectin and ICAM-1 in human endothelial cells

Strindhall, Jan

doi:10.1016/s0928-8244(01)00306-6

Cited by 12 publications

(36 citation statements)

References 13 publications

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“…For instance, Noel et al (16) (32). In addition, nonviable clinical isolates of S. aureus did not elicit expression of endothelial cell E-selectin or ICAM-1 (27) or TF (32). In contrast, M type 3 GAS are potent inducers of TF synthesis in endo- FIG.…”

Section: Discussionmentioning

confidence: 97%

M Type 1 and 3 Group A Streptococci Stimulate Tissue Factor-Mediated Procoagulant Activity in Human Monocytes and Endothelial Cells

2003

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Streptococcal toxic shock syndrome (StrepTSS) is an invasive infection characterized by marked coagulopathy, multiple organ failure, and rapid tissue destruction and is strongly associated with M type 1 and 3 group A streptococci (GAS). Initiation of the coagulation cascade with formation of microvascular thrombi contributes to multiple organ failure in human cases of gram-negative bacteremia; however, little is known regarding the mechanism of coagulopathy in StrepTSS. Thus, we investigated the abilities of several strains of M type 1 and 3 GAS isolated from human cases of StrepTSS to stimulate production of tissue factor (TF), the principal initiator of coagulation in vivo. Washed, killed M type 1 and 3 GAS, but not M type 6 GAS, elicited high-level TF-mediated procoagulant activity from both isolated human monocytes and cultured human umbilical vein endothelial cells. M type 1 GAS consistently elicited higher levels of TF from monocytes than did M type 3 GAS. GAS-induced TF synthesis in monocytes did not correlate with production of tumor necrosis factor alpha or interleukin-8. Conversely, M type 3 GAS were consistently more potent than M type 1 GAS in stimulating endothelial cell TF synthesis. These results demonstrate that (i) M type 1 and 3 strains of GAS are potent inducers of TF synthesis, (ii) GAS-induced TF synthesis is not simply an epiphenomenon of cytokine generation, and (iii) induction of TF in endothelial cells and monocytes may be M type specific. In total, these findings suggest that a novel interaction between GAS and host cells contributes to the observed coagulopathy in StrepTSS.

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Section: Discussionmentioning

confidence: 97%

M Type 1 and 3 Group A Streptococci Stimulate Tissue Factor-Mediated Procoagulant Activity in Human Monocytes and Endothelial Cells

2003

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“…E. coli and S. aureus up-regulate ICAM-1 expression on endothelial cells in vitro (28,29), and ICAM-1 expression on endothelial cells is increased in malaria and in sepsis, both in vivo and postmortem (30 -34).…”

Section: Discussionmentioning

confidence: 99%

Malaria and bacterial sepsis: Similar mechanisms of endothelial apoptosis and its prevention in vitro*

Hemmer

Vogt

Unverricht

et al. 2008

Critical Care Medicine

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“…The aims of this study were (i) to investigate a number of earlier collected clinical isolates of S. aureus for their ability to induce expression of cytokines in EC using an infection assay previously described [16] and (ii) to study whether there is any correlation between expression of the different cytokines and possession of certain virulence determinants in S. aureus .…”

Section: Introductionmentioning

confidence: 99%

Clinical Isolates of Staphylococcus aureus Vary in Ability to Stimulate Cytokine Expression in Human Endothelial Cells

et al. 2005

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Human umbilical vein endothelial cells (HUVEC) were infected for 24 h with 18 well-characterized Staphylococcus aureus isolates, and the supernatants from infected HUVEC were analysed for interleukin (IL)-1b, tumour necrosis factoralpha, IL-6, IL-8, IL-10, IL-12p70, growth-related oncogene (GRO)-a, granulocyte macrophage colony-stimulating factor (GM-CSF) and regulated upon activation, normal T cell expressed and secreted (RANTES) by immunoassay. All staphylococcal isolates induced the expression of IL-6, IL-8, GRO-a, GM-CSF and RANTES. The magnitude of cytokine expression varied between isolates. Staphylococcus aureus inducing high expression of one of these cytokines also showed simultaneous high expression of the other four, indicating a common mechanism for the ability of individual S. aureus to induce expression of these cytokines. No direct correlation between cytokine expression and adhesion of S. aureus to HUVEC was observed, indicating that bacterial properties besides adhesion contribute to the activation of HUVEC.

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Variations among clinical isolates of Staphylococcus aureus to induce expression of E-selectin and ICAM-1 in human endothelial cells

Cited by 12 publications

References 13 publications

M Type 1 and 3 Group A Streptococci Stimulate Tissue Factor-Mediated Procoagulant Activity in Human Monocytes and Endothelial Cells

M Type 1 and 3 Group A Streptococci Stimulate Tissue Factor-Mediated Procoagulant Activity in Human Monocytes and Endothelial Cells

Malaria and bacterial sepsis: Similar mechanisms of endothelial apoptosis and its prevention in vitro*

Clinical Isolates of Staphylococcus aureus Vary in Ability to Stimulate Cytokine Expression in Human Endothelial Cells

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