Variations during leaf development of the relative amounts of two bean (Phaseolus vulgaris) chloroplast tRNAsPhe which differ in their minor nucleotide content

Pfitzinger, H.; Maréchal-Drouard, Laurence; Pillay, D.T.N.; Weil, Jacques-Henry; Guillemaut, Pierre

doi:10.1007/bf00019393

Cited by 14 publications

(6 citation statements)

References 20 publications

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“…2A, lanes L and D; and different exposures of the same gel (data not shown)), is about 1:4 for dark-grown as well as light-grown plants. The level of base modification ofisogenic tRNAs may also vary during leaf development [26]: 15 ~o of chloroplast tRNA Ph~ from etiolated leaves are undermodified at specific positions, whereas the level rises to 60~o in green leaves and decreases again to 9~o during senescence, thus corroborating the finding that some base modifications are more prevalent in resting tissue than in rapidly dividing cells.…”

Section: Sequence and Base Modifications Of T R N A Aspsupporting

confidence: 74%

Chloroplast tRNAAsp: nucleotide sequence and variation of in vivo levels during plastid maturation

1992

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Two chloroplast tRNA(Asp) species from barely were purified by chromatography on benzoylated DEAE-cellulose and sequenced. They differ in the modification at position 34, where queuosine (Q) is present in one of the species. The same chromatographic procedure yielded only one tRNA(Glu) species, corroborating the assumption that the same tRNA(Glu) species participates in both protein and chlorophyll biosynthesis. The level of tRNA(Glu) remains unchanged after light treatment of etiolated seedlings, whereas the amount of tRNA(Asp) decreases to about 50% relative to the level of dark-grown plants.

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Section: Sequence and Base Modifications Of T R N A Aspsupporting

confidence: 74%

Chloroplast tRNAAsp: nucleotide sequence and variation of in vivo levels during plastid maturation

1992

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“…corresponding to a given mitochondrial tRNA after twodimensional polyacrylamide gel electrophoresis (MarechalDrouard et al, 1990a). Such structural differences have been described in more detail for bean mitochondrial tRNATYr and chloroplast tRNAPhe (Marechal et al, 1985a;Pfitzinger et al, 1990). For the latter, the variations correlate with leaf development (Pfitzinger et al, 1990).…”

Section: Resultsmentioning

confidence: 96%

In vivo import of a normal or mutagenized heterologous transfer RNA into the mitochondria of transgenic plants: towards novel ways of influencing mitochondrial gene expression?

1992

Trends in Genetics

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Evidence that nuclear-encoded RNAs are present inside mitochondria has been reported from a wide variety of organisms, and is presumed to be due to import of specific cytosolic RNAs. In plants, the first examples were the mitochondrial leucine transfer RNAs of bean. In all cases, the evidence is circumstantial, based on hybridization of the mitochondrial RNAs to nuclear and not mitochondrial DNA. Here we show that transgenic potato plants carrying a leucine tRNA gene from bean nuclear DNA contain RNA transcribed from the introduced gene both in the cytosol and inside mitochondria, providing proof that the mitochondrial leucine tRNA is derived from a nuclear gene and imported into the mitochondria. The same bean gene carrying a 4 bp insertion in the anticodon loop was also expressed in transgenic potato plants and the transcript found to be present inside mitochondria, suggesting that this natural RNA import system could eventually be used to introduce foreign RNA sequences into mitochondria.

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“…On the other hand the G3:U70 base-pair in tRNA Ala and the anticodon in tRNA Phe were confirmed as major determinants also in plant tRNAs (116). In a more physiological perspective, the relative amounts of two chloro-tRNA Phe isoacceptors were shown to vary during leaf development (115). New Technological Developments at the Heart of tRNA Science Significant novelties came from work combining interconnected approaches (biochemical, cellular, dynamic, enzymologic, evolutionary, genetic, metabolic, medical, phylogenetic, and/or structural) with adapted technologies allowing easy access to the structures and functionalities of the macromolecules.…”

Section: A Stimulating Start At Ibmpmentioning

confidence: 95%

“…On the other hand the G3:U70 base‐pair in tRNA Ala and the anticodon in tRNA Phe were confirmed as major determinants also in plant tRNAs . In a more physiological perspective, the relative amounts of two chloro‐tRNA Phe isoacceptors were shown to vary during leaf development .…”

Section: –1994: a Troubled But Scientifically Rich Periodmentioning

confidence: 97%

“…Research activities concerned various plant models with focus on mitochondria and chloroplasts . Results are illustrated by studies based on sequencing bean tRNAs and a precursory work on tRNAs from Arabidopsis thaliana . Thus, Gm18 in the D‐loop is the only difference between the bean cyto‐ and mito‐tRNA 2 Leu .…”

Section: –1994: a Troubled But Scientifically Rich Periodmentioning

confidence: 99%

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History of tRNA research in strasbourg

Florentz

Giegé

2019

IUBMB Life

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The tRNA molecules, in addition to translating the genetic code into protein and defining the second genetic code via their aminoacylation by aminoacyl‐tRNA synthetases, act in many other cellular functions and dysfunctions. This article, illustrated by personal souvenirs, covers the history of ~60 years tRNA research in Strasbourg. Typical examples point up how the work in Strasbourg was a two‐way street, influenced by and at the same time influencing investigators outside of France. All along, research in Strasbourg has nurtured the structural and functional diversity of tRNA. It produced massive sequence and crystallographic data on tRNA and its partners, thereby leading to a deeper physicochemical understanding of tRNA architecture, dynamics, and identity. Moreover, it emphasized the role of nucleoside modifications and in the last two decades, highlighted tRNA idiosyncrasies in plants and organelles, together with cellular and health‐focused aspects. The tRNA field benefited from a rich local academic heritage and a strong support by both university and CNRS. Its broad interlinks to the worldwide community of tRNA researchers opens to an exciting future. © 2019 IUBMB Life, 2019 © 2019 IUBMB Life, 71(8):1066–1087, 2019

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Variations during leaf development of the relative amounts of two bean (Phaseolus vulgaris) chloroplast tRNAsPhe which differ in their minor nucleotide content

Cited by 14 publications

References 20 publications

Chloroplast tRNAAsp: nucleotide sequence and variation of in vivo levels during plastid maturation

Chloroplast tRNAAsp: nucleotide sequence and variation of in vivo levels during plastid maturation

In vivo import of a normal or mutagenized heterologous transfer RNA into the mitochondria of transgenic plants: towards novel ways of influencing mitochondrial gene expression?

History of tRNA research in strasbourg

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