Variations in binding of [3H]5-HT to cortical membranes during the female rat estrous cycle

Uphouse, Lynda; Williams, Judy; Eckols, Kris; Sierra, Victor

doi:10.1016/0006-8993(86)90093-4

Cited by 39 publications

(8 citation statements)

References 37 publications

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“…Studies have shown that, during estrus, 5-HT exhibits changes in synthesis, release, reuptake and catabolism (Biegon, Bercovitz & Samuel, 1980;Uphouse et al, 1986;Gundlah, Simon & Auerbach, 1998), and fluctuations in this indolamine are correlated with the action of estrogen and progesterone (Kueng, Wirz-Justice & Chappuis-Arndt, 1976;Di Paolo et al, 1983;Renner, Krey & Luine, 1987;Gereau, Kedzie & Renner, 1993;Famer et al, 1996). Some authors report variations in 5-HT extracellular concentrations in diestrus, the higher concentrations being found in the hypothalamus (Gundlah et al, 1998;Maswood et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Influence of gender and estrous cycle in the forced swim test in rats.

Gouvêia¹,

Afonseca²,

Maximino³

et al. 2008

Psychology & Neuroscience

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The present work aimed at studying the influence of the estrous cycle in the forced swim test, an animal model of depression. For this, 44 male and female Wistar rats were divided into five groups according to the hormonal state in the first day of the study: metaestrus (N = 12), diestrus (N = 8), proestrus (N = 7), estrous (N = 6) and males (N = 11). They were housed in groups of five, with water and food ad libitum under a 12/12 h light/dark cycle. Females were screened daily for the estrous cycle. The animals were subjected to two swimming sessions in a glass cylinder with water up to 15 cm at 28±2º C. The data of the first five minutes of a 15-min first session were compared to those of a 5-min second session 24 h later. The results indicate that the latency to the first immobility was substantially reduced in the second session and was longer for females in diestrus and proestrus in the first session. The results also indicate that females in diestrus and proestrus exhibited less immobility than males in the first session; females in diestrus also exhibited less immobility than females in metaestrus. Females in metaestrus and diestrus, as well as males, did not present the decrease in total immobility times in the second session. The present results are analyzed in terms of differential effects of progesterone and estrogen on a learning component and an affective component.

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Section: Discussionmentioning

confidence: 99%

Influence of gender and estrous cycle in the forced swim test in rats.

Gouvêia¹,

Afonseca²,

Maximino³

et al. 2008

Psychology & Neuroscience

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“…tion, including the estrous cycle (Myer and Quey, 1975;Kueng et al, 1976;Biegon et al, 1980;Uphouse et al, 1986;Desan et al, 1988), pregnancy, and the postpartum period (Greengrass and Tongue, 1974;Desan et al, 1988). Furthermore, E and/or P treatment to ovariectomized (OVX) rats has been shown to enhance 5-HT synthesis, turnover, and receptor binding in numerous brain regions (Crowley et al, 1979;Cone et al, 1981;Biegon et al, 1983;Di Paolo et al, 1983;Chomicka, 1986;Cohen and Wise, 1988;Mendelson et al, 1993;Sumner and Fink, 1995).…”

mentioning

confidence: 99%

Immunocytochemical localization of nuclear estrogen receptors and progestin receptors within the rat dorsal raphe nucleus

Alves¹,

Weiland²,

Hayashi³

et al. 1998

J. Comp. Neurol.

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Estradiol and progesterone modulate central serotonergic activity; however, the mechanism(s) of action remain unclear. Recently, estradiol-induced progestin receptors (PRs) have been localized within the majority of serotonin (5-HT) neurons in the female macaque dorsal raphe nucleus (DRN; Bethea [1994] Neuroendocrinology 60:50-61). In the present study, we investigated whether estrogen receptors (ERs) and/or PRs exist within 5-HT and/or non-5-HT cells in the female and male rat DRN and whether estradiol treatment alters the expression of these receptors. Young adult female and male Sprague-Dawley rats were gonadectomized, and 1 week later, half of the animals received a subcutaneous Silastic implant of estradiol-17beta. Animals were transcardially perfused 2 days later with acrolein and paraformaldehyde, and sequential dual-label immunocytochemistry was performed on adjacent sections by using either a PR antibody or an ERalpha antibody. This was followed by an antibody to either the 5-HT-synthesizing enzyme, tryptophan hydroxylase (TPH), or to the astrocytic marker, glial fibrillary acidic protein (GFAP). Cells containing immunoreactivity (ir) for nuclear ERs or PRs were identified within the rat DRN in a region-specific distribution in both sexes. No colocalization of nuclear ER-ir or PR-ir with cytoplasmic TPH-ir or GFAP-ir was observed in either sex or treatment, indicating that the steroid target cells are neither 5-HT neurons nor astrocytes. Females were found to have approximately 30% more PR-labeled cells compared with males throughout the DRN (P < 0.05), but no sex difference was detected in the number of neurons demonstrating ER-ir. In both sexes, 2 days of estradiol exposure decreased the number of cells with ER-ir, whereas it greatly increased the number of cells containing PR-ir in several DRN regions (P < 0.005). Collectively, these findings demonstrate the existence of nonserotonergic cells that contain nuclear ERs or PRs within the female and male rat DRN, including estradiol-inducible PRs. These findings point to a species difference in ovarian steroid regulation of 5-HT activity between the macaque and the rat, perhaps transsynaptically via local neurons in the rat brain.

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“…The studies reported were performed on 4-6-weekold female rats because development of streptococcal cell wall arthritis in these strains has been validated and optimized under these conditions. Many potential interacting neurohormonal systems, including the hypothalamic-pituitary-gonadal (HPG) axis can, and probably do, influence the 5-HT system (Uphouse et al, 1986;Williams et al, 1988). In the present article, we have observed differential 5-HT systems in these strains, without detailing the many potential neuroendocrine causes for these differences.…”

Section: Discussionmentioning

confidence: 99%

Hippocampal 8‐[³H]Hydroxy‐2‐(Di‐n‐Propylamino)Tetralin Binding Site Densities, Serotonin Receptor (5‐HT_1A) Messenger Ribonucleic Acid Abundance, and Serotonin Levels Parallel the Activity of the Hypothalamopituitary‐Adrenal Axis in Rat

Burnet¹,

Mefford

Smith³

et al. 1992

Journal of Neurochemistry

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We have previously demonstrated that susceptibility of the Lewis rat to inflammatory disease, compared with the relatively resistant Fischer F344/N rat, is related to a hyporesponsive hypothalamopituitary-adrenal axis to inflammatory and other stress mediators. Because serotonin (5-HT) and the 5-HT1A receptor are important stimulators of this axis, we have investigated the levels of 8-[3H]-hydroxy-2,3-(di-n-propylamino)tetralin binding sites, 5-HT1A mRNA, 5-HT, and 5-hydroxyindoleacetic acid in various brain regions of Lewis, outbred Harlan Sprague Dawley, and Fischer F344/N rats. Lewis rats expressed significantly fewer hippocampal and frontal cortical 8-[3H]-hydroxy-2,3-(di-n-propylamino)tetralin binding sites and less 5-HT1A mRNA than Harlan Sprague Dawley and Fischer F344/N rats. Adrenalectomy increased the number of 8-[3H]hydroxy-2,3-(di-n-propylamino)tetralin binding sites and 5-HT1A mRNA expression in the hippocampus of all three strains. Levels of hippocampal 5-HT in Fischer F344/N rats were significantly greater than levels detected in the same regions from Lewis and Harlan Sprague Dawley rats. Hypothalamic 5-HT and 5-hydroxyindoleacetic acid levels in Harlan Sprague Dawley rats were higher than the same area from the other two strains. Adrenalectomy increased the levels of 5-hydroxyindoleacetic acid in the hypothalamus of all three strains. We conclude that hippocampal 5-HT1A receptor densities and 5-HT levels in the rat parallel the activity and responsiveness of the hypothalamopituitary-adrenal axis.

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Variations in binding of [3H]5-HT to cortical membranes during the female rat estrous cycle

Cited by 39 publications

References 37 publications

Influence of gender and estrous cycle in the forced swim test in rats.

Influence of gender and estrous cycle in the forced swim test in rats.

Immunocytochemical localization of nuclear estrogen receptors and progestin receptors within the rat dorsal raphe nucleus

Hippocampal 8‐[³H]Hydroxy‐2‐(Di‐n‐Propylamino)Tetralin Binding Site Densities, Serotonin Receptor (5‐HT_1A) Messenger Ribonucleic Acid Abundance, and Serotonin Levels Parallel the Activity of the Hypothalamopituitary‐Adrenal Axis in Rat

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Variations in binding of [3H]5-HT to cortical membranes during the female rat estrous cycle

Cited by 39 publications

References 37 publications

Influence of gender and estrous cycle in the forced swim test in rats.

Influence of gender and estrous cycle in the forced swim test in rats.

Immunocytochemical localization of nuclear estrogen receptors and progestin receptors within the rat dorsal raphe nucleus

Hippocampal 8‐[3H]Hydroxy‐2‐(Di‐n‐Propylamino)Tetralin Binding Site Densities, Serotonin Receptor (5‐HT1A) Messenger Ribonucleic Acid Abundance, and Serotonin Levels Parallel the Activity of the Hypothalamopituitary‐Adrenal Axis in Rat

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Hippocampal 8‐[³H]Hydroxy‐2‐(Di‐n‐Propylamino)Tetralin Binding Site Densities, Serotonin Receptor (5‐HT_1A) Messenger Ribonucleic Acid Abundance, and Serotonin Levels Parallel the Activity of the Hypothalamopituitary‐Adrenal Axis in Rat