Variations in Cell Surfaces of Estrogen Treated Breast Cancer Cells Detected by A Combined Instrument for Far‐Field and Near‐Field Microscopy

Perner, Petra; Rapp, Alexander; Dressler, Cathrin; Wollweber, Leo; Beuthan, J.; Greulich, Karl-Otto; Hausmann, Michael

doi:10.1155/2002/132504

Cited by 9 publications

(5 citation statements)

References 52 publications

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“…To the best of our knowledge, this is the first report describing the effects of 20-nm AuNPs in combination with E 2 (1×10 −9 M) on the cell surface roughness of any cell line. Perner et al (2002) demonstrated that the surfaces of ER-positive human breast cancer cells (T-47D) became increasingly jagged at physiological E 2 concentrations (5×10 −9 and 5×10 −7 M), as detected by an increase in membrane height in near-field light transmission images 68. MCF-7 cells have been reported to exhibit a more disorganized filamentous cytoskeleton structure, increased membrane roughness, decreased viscoelastic properties (elasticity and viscosity) and softer and more fluid membranes compared to benign breast cells MCF-10A 60.…”

Section: Resultsmentioning

confidence: 99%

<p>Gold nanoparticle uptake is enhanced by estradiol in MCF-7 breast cancer cells</p>

Lara-Cruz

Jiménez-Salazar

Arteaga

et al. 2019

IJN

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Purpose: In the present study, we investigated the effects of 17β-estradiol (E 2 ) on membrane roughness and gold nanoparticle (AuNP) uptake in MCF-7 breast cancer cells. Methods: Estrogen receptor (ER)-positive breast cancer cells (MCF-7) were exposed to bare 20 nm AuNPs in the presence and absence of 1×10 −9 M E 2 for different time intervals for up to 24 hrs. The effects of AuNP incorporation and E 2 incubation on the MCF-7 cell surface roughness were measured using atomic force microscopy (AFM). Endocytic vesicle formation was studied using confocal laser scanning microscopy (CLSM). Finally, the results were confirmed by hyperspectral optical microscopy. Results: High-resolution AFM images of the surfaces of MCF-7 membranes (up to 250 nm 2 ) were obtained. The incubation of cells for 12 hrs with AuNP and E 2 increased the cell membrane roughness by 95% and 30% compared with the groups treated with vehicle (ethanol) or AuNPs only, respectively. This effect was blocked by an ER antagonist (7α,17β-[9-[(4,4,5,5,5-Pentafluoropentyl)sulfinyl]nonyl]estra-1,3,5(10)-triene-3,17-diol [ICI] 182,780). Higher amounts of AuNPs were localized inside MCF-7 cells around the nucleus, even after 6 hrs of E 2 incubation, compared with vehicle-treated cells. Endolysosome formation was induced by E 2 , which may be associated with an increase in AuNP-uptake. Conclusions: E 2 enhances AuNP incorporation in MCF-7 cells by modulating of plasma membrane roughness and inducing lysosomal endocytosis. These findings provide new insights into combined nanotherapies and hormone therapies for breast cancer.

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Section: Resultsmentioning

confidence: 99%

<p>Gold nanoparticle uptake is enhanced by estradiol in MCF-7 breast cancer cells</p>

Lara-Cruz

Jiménez-Salazar

Arteaga

et al. 2019

IJN

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“…However, storage of samples is not discussed extensively in the literature and this is clearly important when considering the results presented here. The SNOM technique is likely to become an essential research tool in the fields of medicine and biology, indeed it has recently been applied to imaging breast cancer cells (Perner et al ., 2002) to understand the morphological effects of high dosages of 17beta‐estradiol (E‐2) using light transmission through the samples.…”

Section: Discussionmentioning

confidence: 99%

Chromosome morphology after long‐term storage investigated by scanning near‐field optical microscopy

Baylis

Doak

Parry

et al. 2006

Journal of Microscopy

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SummaryFluorescence in situ hybridization coupled with far-field fluorescence microscopy is a commonly used technique to visualize chromosomal aberrations in diseased cells. To obtain the best possible results, chromatin integrity must be preserved to ensure optimal hybridization of fluorescence in situ hybridization probes. However, biological samples are known to degrade and storage conditions can be critical. This study concentrates its investigation on chromatin stability as a function of time following fluorescence in situ hybridization type denaturing protocols. This issue is extremely important because chromatin integrity affects the fluorescence response of the chromosome. To investigate this, metaphase chromosome spreads of human lymphocytes were stored at both − 20 and − 80 ° C, and were then imaged using scanning near-field optical microscopy over a nine month period. Using the scanning nearfield optical microscope's topography mode, chromosome morphology was analysed before and after the application of fluorescence in situ hybridization type protocols, and then as a function of storage time. The findings revealed that human chromosome samples can be stored at − 20 ° C for short periods of time ( ∼ several weeks), but storage over 3 months compromises chromatin stability. Topography measurements clearly show the collapse of the stored chromatin, with variations as large as 60 nm across a chromosome. However, storage at − 80 ° C considerably preserved the integrity with variations in topography significantly reduced. We report studies of the fluorescent response of stored chromosomes using scanning near-field optical microscopy and their importance for gaining further understanding of chromosomal aberrations.

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“…total internal reflection fluorescence (TIRF) microscopy (TIRFM) [118,119] or near-field scanning optical microscopy (SNOM, NSOM) [120,121], belong to the first techniques breaking the diffraction limit by novel techniques working in the optical near field of fine crystal tips probing the specimen without an microscope objective lens. Unfortunately, near-field techniques are technically restricted to the visualization of surfaces of cells, membranes or isolated organelles [122][123][124].…”

Section: Super-resolution Microscopymentioning

confidence: 99%

Super-Resolution Radiation Biology: From Bio-Dosimetry towards Nano-Studies of DNA Repair Mechanisms

Lee¹,

Hausmann²

2021

DNA - Damages and Repair Mechanisms

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Past efforts in radiobiology, radio-biophysics, epidemiology and clinical research strongly contributed to the current understanding of ionizing radiation effects on biological materials like cells and tissues. It is well accepted that the most dangerous, radiation induced damages of DNA in the cell nucleus are double strand breaks, as their false rearrangements cause dysfunction and tumor cell proliferation. Therefore, cells have developed highly efficient and adapted ways to repair lesions of the DNA double strand. To better understand the mechanisms behind DNA strand repair, a variety of fluorescence microscopy based approaches are routinely used to study radiation responses at the organ, tissue and cellular level. Meanwhile, novel super-resolution fluorescence microscopy techniques have rapidly evolved and become powerful tools to study biological structures and bio-molecular (re-)arrangements at the nano-scale. In fact, recent investigations have increasingly demonstrated how super-resolution microscopy can be applied to the analysis of radiation damage induced chromatin arrangements and DNA repair protein recruitment in order to elucidate how spatial organization of damage sites and repair proteins contribute to the control of repair processes. In this chapter, we would like to start with some fundamental aspects of ionizing radiation, their impact on biological materials, and some standard radiobiology assays. We conclude by introducing the concept behind super-resolution radiobiology using single molecule localization microscopy (SMLM) and present promising results from recent studies that show an organized architecture of damage sites and their environment. Persistent homologies of repair clusters indicate a correlation between repair cluster topology and repair pathway at a given damage locus. This overview over recent investigations may motivate radiobiologists to consider chromatin architecture and spatial repair protein organization for the understanding of DNA repair processes.

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Variations in Cell Surfaces of Estrogen Treated Breast Cancer Cells Detected by A Combined Instrument for Far‐Field and Near‐Field Microscopy

Cited by 9 publications

References 52 publications

<p>Gold nanoparticle uptake is enhanced by estradiol in MCF-7 breast cancer cells</p>

<p>Gold nanoparticle uptake is enhanced by estradiol in MCF-7 breast cancer cells</p>

Chromosome morphology after long‐term storage investigated by scanning near‐field optical microscopy

Super-Resolution Radiation Biology: From Bio-Dosimetry towards Nano-Studies of DNA Repair Mechanisms

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