Variations in chemical fingerprints and major flavonoid contents from the leaves of thirty‐one accessions of Hibiscus sabdariffa L.

Abstract

The leaves of Hibiscus sabdariffa L. have been used as traditional folk medicines for treating high blood pressure and fever. There are many accessions of H. sabdariffa L. throughout the world. To assess the chemical variations of 31 different accessions of H. sabdariffa L., fingerprinting analysis and quantitation of major flavonoids were performed by high‐performance liquid chromatography (HPLC). The HPLC method was validated for linearity, sensitivity, precision, repeatability and accuracy. A quadrupole‐time‐of‐flight mass spectrometry (Q‐TOF‐MS) was applied for the characterization of major compounds. A total of 9 compounds were identified, including 6 flavonoids and 3 phenolic acids. In the fingerprint analysis, similarity analysis (SA) and principal component analysis (PCA) were used to differentiate the 31 accessions of H. sabdariffa L. Based on the results of PCA and SA, the samples No. 15 and 19 appeared much different from the main group. The total content of five flavonoids varied greatly among different accessions, ranging from 3.35 to 23.30 mg/g. Rutin was found to be the dominant compound and the content of rutin could contribute to chemical variations among different accessions. This study was helpful to understand the chemical variations between different accessions of H. sabdariffa L., which could be used for quality control. © 2015 The Authors Biomedical Chromatography Published by John Wiley & Sons Ltd.

Phenolic Compounds in Extracts of Hibiscus acetosella (Cranberry Hibiscus) and Their Antioxidant and Antibacterial Properties

“…A previous study on H. acetosella , investigating a different variety using a different extraction method, reported TPC and TFC levels of 1730 and 775 mg/100 g, respectively, values more than three times those found in the present study [ 19 ]. Phenolic compounds play an important role in the adaptation of plants to the environment, and their content is determined by the origin, harvesting time, and cultivation conditions [ 3 , 20 ]. Previous data has shown that the TPC and TFC contents can vary by approximately three times according to the region where the plants are collected [ 21 , 22 ].…”

“…In the traditional medicine of western and central Africa, decoction drinks have been prepared from extracts of the leaves and shoots because of their anti-anemic and antipyretic properties [ 1 ]. The presence of a wide variety of biochemical compounds, such as polyphenols, flavonoids, and anthocyanins have been reported in Hibiscus species [ 2 , 3 ]. Two examples, H. cannabinus and H. sabdariffa , have been studied most regarding the relationship between their biochemical compounds in the plant leaves and their biofunctional activity [ 4 , 5 , 6 , 7 ], while H. acetosella has been much less studied.…”

Chemical Fingerprint and Multicomponent Quantitative Analysis for the Quality Evaluation of Cyclocarya paliurus Leaves by HPLC–Q–TOF–MS

“…It has been widely used in the identification of authenticity, differentiation of origin and evaluation of quality of traditional Chinese medicine and other botanical products [ 25 , 26 , 27 , 28 ]. At present, this method has been adopted by the World Health Organization and other authorities as a strategy for quality assessment of botanical products [ 18 , 29 , 30 ]. Among the chromatographic fingerprinting applied to the authentication and qualitative evaluation of botanical products over the past decade, high-performance liquid chromatography (HPLC) fingerprint emerges to be the most widely used method due to its convenience and efficiency [ 25 , 31 , 32 ].…”

Utilisation des feuilles de <i>Ficus sycomorus</i> L. (Moraceae) dans la prévention de l’hypercoagulation chez les drépanocytaires: identification de composés phénoliques potentiellement anticoagulant et antiagrégant plaquettaire

“…Les différents composés ont été repérés et identifiés grâce à leur temps de rétention avec une détection par absorption dans l'UV-vis. Les données enregistrées ont été analysées à l'aide du logiciel MssLynx 4.1 (de Rijke et al, 2006;Wang et al, 2016).…”