Variations in DNA Synthesis and Mitotic Indices in Hepatocytes and Sinusoid Litoral Cells of Adult Intact Male Mouse Along a Orcadian Time Span

Surur, J. M.; Moreno, Félix R.; Badrán, Amado F.; Llanos, J. M. Echave

doi:10.3109/07420528509055555

Cited by 17 publications

(9 citation statements)

References 15 publications

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“…[3][4][5][6]14,16] In this sense, our experiment corroborates a conspicuous circadian rhythm of DNAs with a peak at 08:00 hr in PPH and PVH of intact young mice. This result is consistent with the cell cycle events in which the peak of DNAs occur at 08:00 hr (S phase) and the peak of mitotic activity at 12:00 hr.…”

Section: Discussionsupporting

confidence: 88%

DNA Synthesis in Periportal and Perivenous Hepatocytes of Intact and Hepatectomized Young Mice

Fernández-Blanco

Inda

Errecalde

2014

Journal of Immunoassay and Immunochemistry

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DNA synthesis of hepatocytes in two areas of Intact and Hepatectomized young mice liver along a circadian period was studied. DNA synthesis was significantly different at all analyzed time points in Intact and Hepatectomized animals. Differences between periportal and perivenous hepatocytes were found in hepatectomized animals at 04/42 and 08/46 hr of day/hour post-hepatectomy. DNAs peak in periportal hepatocytes regenerating liver occurs 4 hr earlier than in perivenous hepatocytes, probably reflecting their shorter G1 phase. Besides, daily mean values of regenerating livers were higher than those observed in Intact animals, as a consequence of surgical removal.

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Section: Discussionsupporting

confidence: 88%

DNA Synthesis in Periportal and Perivenous Hepatocytes of Intact and Hepatectomized Young Mice

Fernández-Blanco

Inda

Errecalde

2014

Journal of Immunoassay and Immunochemistry

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“…(3) In adult rat livers, most cells remain in Go ; nevertheless, the low level of corticosterone in the morning does not induce any important proliferative wave (Surur et al, 1985) compared to liver regeneration.…”

Section: Discussionmentioning

confidence: 99%

Circadian synchronization of liver regeneration in adult rats: the role played by adrenal hormones

et al. 1989

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The role played by the adrenal hormones in the regulation of liver proliferation in adult rats was investigated under various experimental conditions.In untreated control groups, cell growth was very low and endogenous corticosterone levels showed a clearly-defined circadian rhythm with a peak in the evening. Adrenalectomy depressed the level of endogenous corticosterone immediately and the growth rate of the liver increased significantly. We were able to prevent this effect by repeated injections of corticosterone at physiological doses.After a 1/3 hepatectomy and a sham-operation, the corticosterone blood level maintained its normal circadian pattern with the exception of a transient increase during the first two post-operative hours. After a hepatectomy of this kind, a negative correlation was found to exist between the adrenal hormone level and the waves of DNA synthesis; the subsequent mitoses appeared in two successive circadian waves of decreasing amplitude, a maximum value being reached in the morning. In rats submitted to a 1/3 hepatectomy and an adrenalectomy simultaneously, the endogenous corticosterone level fell significantly after a post-operative peak. The regenerating pattern was completely different from that induced by 1/3 hepatectomy alone. The rise in the labelling index began earlier and rose to significantly higher values; it was then followed by a single large mitotic wave without any circadian rhythm.These results favour the hypothesis that adrenal hormones have a significant effect on the negative control of liver regeneration. Circadian changes in the corticosterone level were responsible for the nycthemeral pattern observed in the regenerating liver after a partial hepatectomy. The results show a marked inhibition of the G,-S transition, particularly in the evening, when the endogenous corticosterone concentration was at its highest. Also discussed is the relationship between corticoids and 'chalones', which synergetically inhibit the passage from Go into the cell cycle.We have previously shown that the circadian synchronization of hepatic cell proliferation and specific enzyme activities are triggered from the 20th day of life onwards (Barbason, Van Cantfort & Houbrechts, 1974). This corresponds to the maturation and the circadian activity of

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“…EGFR has been implicated in the control of liver regeneration and growth; however, the liver of the adult mouse is mitotically inactive under normal circumstances. Few hepatocytes (0.2 to 3.2% dependent on time of day) or sinusoidal nonparenchymal cells (1.1 to 5.7%) traverse the cell cycle each day (50). This implies that EGFR may have other nonproliferative roles.…”

mentioning

confidence: 98%

Epidermal growth factor receptor plays a role in the regulation of liver and plasma lipid levels in adult male mice

Scheving

Zhang

García

et al. 2014

American Journal of Physiology-Gastrointestinal and Liver Physi

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Dsk5 mice have a gain of function in the epidermal growth factor receptor (EGFR), caused by a point mutation in the kinase domain. We analyzed the effect of this mutation on liver size, histology, and composition. We found that the livers of 12-wk-old male Dsk5 heterozygotes (+/Dsk5) were 62% heavier compared with those of wild-type controls (+/+). The livers of the +/Dsk5 mice compared with +/+ mice had larger hepatocytes with prominent, polyploid nuclei and showed modestly increased cell proliferation indices in both hepatocytes and nonparenchymal cells. An analysis of total protein, DNA, and RNA (expressed relative to liver weight) revealed no differences between the mutant and wild-type mice. However, the livers of the +/Dsk5 mice had more cholesterol but less phospholipid and fatty acid. Circulating cholesterol levels were twice as high in adult male +/Dsk5 mice but not in postweaned young male or female mice. The elevated total plasma cholesterol resulted mainly from an increase in low-density lipoprotein (LDL). The +/Dsk5 adult mouse liver expressed markedly reduced protein levels of LDL receptor, no change in proprotein convertase subtilisin/kexin type 9, and a markedly increased fatty acid synthase and 3-hydroxy-3-methyl-glutaryl-CoA reductase. Increased expression of transcription factors associated with enhanced cholesterol synthesis was also observed. Together, these findings suggest that the EGFR may play a regulatory role in hepatocyte proliferation and lipid metabolism in adult male mice, explaining why elevated levels of EGF or EGF-like peptides have been positively correlated to increased cholesterol levels in human studies.

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Variations in DNA Synthesis and Mitotic Indices in Hepatocytes and Sinusoid Litoral Cells of Adult Intact Male Mouse Along a Orcadian Time Span

Cited by 17 publications

References 15 publications

DNA Synthesis in Periportal and Perivenous Hepatocytes of Intact and Hepatectomized Young Mice

DNA Synthesis in Periportal and Perivenous Hepatocytes of Intact and Hepatectomized Young Mice

Circadian synchronization of liver regeneration in adult rats: the role played by adrenal hormones

Epidermal growth factor receptor plays a role in the regulation of liver and plasma lipid levels in adult male mice

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