Variations in early gut microbiome are associated with childhood eczema

Jin, Sun; Wang, Jingjing; Zhang, Lanying; Mu, Yu; Huang, Kefei; Zhang, Kejian; Cui, Yutao; Li, Sabrina

doi:10.1093/femsle/fnz020

Cited by 14 publications

(15 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1–3 Indeed, a growing body of scientific evidence has established that intestinal dysbiosis during infancy is associated with a number of autoimmune and allergic diseases later in life. 2,3,7,21 Conversely, however, the presence of bifidobacterial species (e.g., B. infantis ) is associated with decreased intestinal dysbiosis and a significantly decreased risk of autoimmune and allergic disease development, 7 including inflammatory conditions such as eczema. 21 Moreover, Bifidobacteriaceae , specifically B. infantis , colonization in infancy has been shown to improve the development of the host immune system, leading to enhanced CD4 T cell responses and increased IgA and IgG titers to vaccines in infancy and at 2 years.…”

Section: Discussionmentioning

confidence: 99%

“…2,3,7,21 Conversely, however, the presence of bifidobacterial species (e.g., B. infantis ) is associated with decreased intestinal dysbiosis and a significantly decreased risk of autoimmune and allergic disease development, 7 including inflammatory conditions such as eczema. 21 Moreover, Bifidobacteriaceae , specifically B. infantis , colonization in infancy has been shown to improve the development of the host immune system, leading to enhanced CD4 T cell responses and increased IgA and IgG titers to vaccines in infancy and at 2 years. 28 Importantly, these same reports showed that reduced vaccine response correlated with greater microbial diversity and increased Enterobacteriaceae abundance, which suggests that impaired immune development may be a consequence of gut dysbiosis and enteric inflammation as seen in control infants here, whereas the infants fed EVC001 exhibited decreased inflammation during this critical window of lymphocyte programming and immune system development.…”

Section: Discussionmentioning

confidence: 99%

“…Evaluation of systemic chronic inflammation in infants has identified that several cytokines (e.g., interleukin (IL)-6, IL-17, IL-22, tumor necrosis factor-α (TNFα), and interferon-γ (IFNγ)) are instrumental in the progression of autoimmune and allergic diseases; 1,3,17–19 however, few reports describe chronic intestinal inflammation in infant populations, which is important given the association with the underlying pathogenesis of autoimmune disease in adults. 20 Moreover, the production of such cytokines has been well established to impair gut barrier function, leading to increased intestinal permeability, which has both short- and long-term consequences, including eczema, Crohn’s disease, ulcerative colitis, and type 1 diabetes, 21–23 as well as drives immunopathogenesis. 24…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Colonization by B. infantis EVC001 modulates enteric inflammation in exclusively breastfed infants

et al. 2019

View full text Add to dashboard Cite

BackgroundInfant gut dysbiosis, often associated with low abundance of bifidobacteria, is linked to impaired immune development and inflammation—a risk factor for increased incidence of several childhood diseases. We investigated the impact of B. infantis EVC001 colonization on enteric inflammation in a subset of exclusively breastfed term infants from a larger clinical study.MethodsStool samples (n = 120) were collected from infants randomly selected to receive either 1.8 × 1010 CFU B. infantis EVC001 daily for 21 days (EVC001) or breast milk alone (controls), starting at day 7 postnatal. The fecal microbiome was analyzed using 16S ribosomal RNA, proinflammatory cytokines using multiplexed immunoassay, and fecal calprotectin using ELISA at three time points: days 6 (Baseline), 40, and 60 postnatal.ResultsFecal calprotectin concentration negatively correlated with Bifidobacterium abundance (P < 0.0001; ρ = −0.72), and proinflammatory cytokines correlated with Clostridiaceae and Enterobacteriaceae, yet negatively correlated with Bifidobacteriaceae abundance. Proinflammatory cytokines were significantly lower in EVC001-fed infants on days 40 and 60 postnatally compared to baseline and compared to control infants.ConclusionOur findings indicate that gut dysbiosis (absence of B. infantis) is associated with increased intestinal inflammation. Early addition of EVC001 to diet represents a novel strategy to prevent enteric inflammation during a critical developmental phase.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Colonization by B. infantis EVC001 modulates enteric inflammation in exclusively breastfed infants

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Primers 341f (5′-CCTAYGGGRBGCASCAG-3′) and 806r (5′-GGACTACNNGGGTATCTAAT-3′) were used to amplify the V3-V4 region of bacterial 16S rRNA. Illumina sequencing libraries (Zhang et al, 2019) were prepared using the one-step PCR in a 25-μl reaction mixture containing 25 ng of input DNA, 333 nmol of forward and reverse primers and KAPA HiFi PCR Master Mix (Kapa Biosystems, Boston, MA, USA). PCR was conducted as follows: an initial 3-min enzyme activation step at 95°C; 20 cycles of 15 s at 98°C, 30 s at 50°C, and 40 s at 72°C; and 10 min at 72°C.…”

Section: Methodsmentioning

confidence: 99%

Linggui Zhugan Formula Improves Glucose and Lipid Levels and Alters Gut Microbiota in High-Fat Diet-Induced Diabetic Mice

Zhao

et al. 2019

Front. Physiol.

View full text Add to dashboard Cite

Background: The gut microbiota plays important roles in the occurrence and development of obesity and diabetes through participating in nutrient absorption and metabolism. Microecological regulation is likely to be key to understanding the effects of Chinese medicine. The Linggui Zhugan (LGZG) formula is a well-known Chinese medicine for controlling obesity in the clinic. However, its pharmacological effects and mechanism of action in diabetes require further exploration. Objective: To evaluate the effects of LGZG on body weight, glycemic control, lipid levels, and gut microbiota in high-fat diet-induced diabetic mice. Methods: High-fat diet-induced diabetic mice were subjected to an 8-week protocol of LGZG administration. We then evaluated the pharmacological effects of LGZG and its influence on gut microbes in fecal samples using the 16S rRNA-based microbiome profiling technique. Results: LGZG administration significantly reduced body weight and body fat mass in diabetic mice. Compared with the high-fat diet control group, LGZG favorably influenced blood glucose control, decreased blood glucose levels, and increased glucose tolerance, accompanied by an improvement in lipid metabolism. Furthermore, the global community composition and relative abundance of many taxa differed between mice fed chow or a high-fat diet. As expected, LGZG supplementation altered the general community structure of gut microbiota, the Firmicutes/Bacteroidetes ratio, and the relative abundance of certain bacteria, such as Bacteroides, Lactobacillus, Oscillospira, and Helicobacter . Conclusion: LGZG effectively controlled obesity and relieved insulin resistance, which may be closely related to its impact on gut microbiota.

show abstract

“…The etiology of eczema is complicated, its pathogenesis is unclear, and there is no effective treatment. Relevant studies have reported that the occurrence of infant eczema is linked to the variation of the intestinal microbiome in the early stage [ 11 ], probiotics, breastmilk feeding [ 12 ], the birth season ( spring and summer) [ 13 ] can reduce the development of eczema.…”

Section: Introductionmentioning

confidence: 99%

Can Mixed Strains of Lactobacillus and Bifidobacterium Reduce Eczema in Infants under Three Years of Age? A Meta-Analysis

et al. 2021

View full text Add to dashboard Cite

(1) Background: Whether early supplementation of probiotics to improve intestinal flora can effectively prevent eczema remains a controversial issue. We aimed to investigate the effect of a mixed strain of Lactobacillus and Bifidobacterium on eczema in infants under three years old at present; (2) Methods: We searched the databases of PubMed, Web of Science, and Cochrane Library, as well as National Knowledge Infrastructure (CNKI), WeiPu (VIP), and WanFang Data (WanFang) for randomized controlled trials (RCTs) of probiotics in the prevention of eczema in infants without language restriction. The main outcome was eczema incidence, while adverse events during the intervention constituted the secondary outcome. The random-/fixed-effects model was utilized to calculate the combined relative risk (RR) and 95% confidence interval (CI). The methodological quality of the study was evaluated using the Cochrane “bias risk” tool. According to the initial intervention time, subgroup analysis was carried out, follow-up time, family history, etc.; (3) Results: Nine articles were selected (2093 infants). The Lactobacillus and Bifidobacterium mixed strain could prevent eczema in infants under three years of age compared to the placebo (RR = 0.60; I2 = 67%; p < 0.001). Subgroup analysis revealed that the mixture of two probiotic strains had preventive effects on both infants with positive (RR = 0.53; I2 = 52%; p < 0.001) and negative (RR = 0.69; I2 = 62%; p = 0.02) family history; The follow-up time for ≤12 months (RR = 0.65; I2 = 12%; p = 0.01) and 12–24 months (RR = 0.60; I2 = 79%; p = 0.003), daily dose of probiotics ≤ 1 × 109 and > 1 × 109 colony forming units all can be effective (p < 0.01); Compared with the intervention of infants alone (RR = 0.63; I2 = 63%; p = 0.29), the effect of probiotics mixture at the beginning of pregnancy was more significant (RR = 0.59; I2 = 71%; p < 0.001); Except for the mixture of Lactobacillus rhamnosusGG (LGG) and Bifidobacterium longum (B. longum) (p = 0.18), other subgroups of intervention group can play a preventive effect (p < 0.05); (4) Conclusions: The mixed strain of Lactobacillus and Bifidobacterium can effectively reduce the incidence of eczema in infants under three years old. However, further research is needed to fully understand the exact mechanism of their effect on infant eczema.

show abstract

Variations in early gut microbiome are associated with childhood eczema

Cited by 14 publications

References 37 publications

Colonization by B. infantis EVC001 modulates enteric inflammation in exclusively breastfed infants

Colonization by B. infantis EVC001 modulates enteric inflammation in exclusively breastfed infants

Linggui Zhugan Formula Improves Glucose and Lipid Levels and Alters Gut Microbiota in High-Fat Diet-Induced Diabetic Mice

Can Mixed Strains of Lactobacillus and Bifidobacterium Reduce Eczema in Infants under Three Years of Age? A Meta-Analysis

Contact Info

Product

Resources

About