Variations in gut microbiome and metabolites of dogs with acute diarrhea in poodles and Labrador retrievers

Bai, Huasong; Liu, Tong; Wang, Songjun; Shen, Liya; Wang, Zhanzhong

doi:10.1007/s00203-023-03439-6

Cited by 4 publications

(2 citation statements)

References 35 publications

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“…Undigested starch and sucrose promote bacterial fermentation and gas production, with water diffusion, causing abdominal bloating, pain, and diarrhea. Probiotics such as Bifidobacterium and Lactobacillus can significantly relieve diarrhea by increasing starch and sucrose metabolism (Li et al, 2022;Bai et al, 2023). Therefore, it is highly likely that the promotion of Bifidobacterium and Lactobacillus proliferation by FOS led to improvements in starch and sucrose metabolism, facilitating recovery from functional diarrhea among children.…”

Section: Discussionmentioning

confidence: 99%

Effects of prebiotics on the gut microbiota in vitro associated with functional diarrhea in children

Du,

Li,

et al. 2023

Front. Microbiol.

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PurposeDiarrhea is among the top five causes of morbidity and mortality in children. Dysbiosis of the gut microbiota is considered the most important risk factor for diarrhea. Prebiotics have shown efficacy in treating diarrhea by regulating the balance of the gut microbiota in vivo.MethodsIn this study, we used an in vitro fermentation system to prevent the interference of host-gut microbe interactions during in vivo examination and investigated the effect of fructo-oligosaccharides (FOS) on gut microbiota composition and metabolism in 39 pediatric patients with functional diarrhea.Results16S rRNA sequencing revealed that FOS significantly improved α- and β-diversity in volunteers with pediatric diarrhea (p < 0.05). This improvement manifested as a significant increase (LDA > 2, p < 0.05) in probiotic bacteria (e.g., Bifidobacterium) and a significant inhibition (LDA > 2, p < 0.05) of harmful bacteria (e.g., Escherichia-Shigella). Notably, the analysis of bacterial metabolites after FOS treatment showed that the decrease in isobutyric acid, isovaleric acid, NH3, and H2S levels was positively correlated with the relative abundance of Lachnoclostridium. This decrease also showed the greatest negative correlation with the abundance of Streptococcus. Random forest analysis and ROC curve validation demonstrated that gut microbiota composition and metabolites were distinct between the FOS treatment and control groups (area under the curve [AUC] > 0.8). Functional prediction using PICRUSt 2 revealed that the FOS-induced alteration of gut microbiota was most likely mediated by effects on starch and sucrose metabolism.ConclusionThis study is the first to evince that FOS can modulate gut microbial disorders in children with functional diarrhea. Our findings provide a framework for the application of FOS to alleviate functional diarrhea in children and reduce the use of antibiotics for managing functional diarrhea-induced disturbances in the gut microbiota.

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Section: Discussionmentioning

confidence: 99%

Effects of prebiotics on the gut microbiota in vitro associated with functional diarrhea in children

Du,

Li,

et al. 2023

Front. Microbiol.

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“…The gut microbiome plays an important role in health and disease. In dogs, both acute [ 1 , 2 ] and chronic enteropathy [ 3 , 4 ] have been linked to alterations in the gut microbiome. Therefore, understanding the composition and function of the normal gut microbiome is essential for developing new diagnostic tools and therapeutic approaches for diseases and improving our understanding of their underlying mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Correlation between Targeted qPCR Assays and Untargeted DNA Shotgun Metagenomic Sequencing for Assessing the Fecal Microbiota in Dogs

Sung,

Pilla,

Chen

et al. 2023

Animals

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DNA shotgun sequencing is an untargeted approach for identifying changes in relative abundances, while qPCR allows reproducible quantification of specific bacteria. The canine dysbiosis index (DI) assesses the canine fecal microbiota by using a mathematical algorithm based on qPCR results. We evaluated the correlation between qPCR and shotgun sequencing using fecal samples from 296 dogs with different clinical phenotypes. While significant correlations were found between qPCR and sequencing, certain taxa were only detectable by qPCR and not by sequencing. Based on sequencing, less than 2% of bacterial species (17/1190) were consistently present in all healthy dogs (n = 76). Dogs with an abnormal DI had lower alpha-diversity compared to dogs with normal DI. Increases in the DI correctly predicted the gradual shifts in microbiota observed by sequencing: minor changes (R = 0.19, DI < 0 with any targeted taxa outside the reference interval, RI), mild-moderate changes (R = 0.24, 0 < DI < 2), and significant dysbiosis (R = 0.54, 0.73, and 0.91 for DI > 2, DI > 5, and DI > 8, respectively), compared to dogs with a normal DI (DI < 0, all targets within the RI), as higher R-values indicated larger dissimilarities. In conclusion, the qPCR-based DI is an effective indicator of overall microbiota shifts observed by shotgun sequencing in dogs.

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Effect of calf separation on gut microbiome and fecal metabolome of mother in the captive Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis)

Shah,

Tang,

et al. 2024

Int Microbiol

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Variations in gut microbiome and metabolites of dogs with acute diarrhea in poodles and Labrador retrievers

Cited by 4 publications

References 35 publications

Effects of prebiotics on the gut microbiota in vitro associated with functional diarrhea in children

Effects of prebiotics on the gut microbiota in vitro associated with functional diarrhea in children

Correlation between Targeted qPCR Assays and Untargeted DNA Shotgun Metagenomic Sequencing for Assessing the Fecal Microbiota in Dogs

Effect of calf separation on gut microbiome and fecal metabolome of mother in the captive Yangtze finless porpoise (Neophocaena asiaeorientalis asiaeorientalis)

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