Variations in Lentiviral Gene Expression in Monocyte-derived Macrophages from Naturally Infected Sheep

Chebloune, Yahia; Karr, Bradley M.; Sheffer, Darlene; Leung, Kevin; Narayan, Opendra

doi:10.1099/0022-1317-77-9-2037

Cited by 45 publications

(20 citation statements)

References 25 publications

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“…All animals providing cells for this study came from breeding stock with a confirmed history of absence of CAEV infection by both ELISA serology and PCR examination of peripheral blood leukocytes [5]. Aortic endothelial cells were collected at autopsy from anaesthesied four newborn kids and blood was withdrawn by venipuncture into EDTA as required from multiparous Saanen goats does for preparation of blood leukocytes.…”

Section: Animalsmentioning

confidence: 99%

In vitro infection of aortic endothelial cells by caprine arthritis encephalitis virus enhances in vitro transmigration of peripheral blood leukocytes and modulates their phenotypic expression

et al. 2003

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-Infection of goats by caprine arthritis-encephalitis virus (CAEV) provides a convenient example of the infiltration of various tissues by leukocytes following a natural lentiviral infection. This event is important in determining organ susceptibility and local immunity. Caprine vascular endothelial cells are susceptible to infection by CAEV in vitro, so we have investigated the consequences of this infection on the transmigration of uninfected leukocytes in an in vitro model. After in vitro infection by CAEV or stimulation by TNFa, the endothelial cells allowed the passage of tenfold more leukocytes from uninfected donors than did the uninfected endothelial cells. The transmigrating leukocytes were enriched in CD8 + lymphocytes, and the leukocytes appeared to have been activated during transmigration, as demonstrated by their expression of IL2R, MHC class II antigens and gamma-delta T-lymphocyte markers. CD4 + , CD8 + and B-lymphocytes all proliferated in culture after transmigration. These results suggest that any possible infection or specific stimulation of endothelia in an infected animal could profoundly influence the choice of target organs and could activate the cells involved in local mucosal immune responses. caev / endothelial cell / leukocyte / transmigration / in vitro

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Section: Animalsmentioning

confidence: 99%

In vitro infection of aortic endothelial cells by caprine arthritis encephalitis virus enhances in vitro transmigration of peripheral blood leukocytes and modulates their phenotypic expression

et al. 2003

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“…Sampled animals were first tested serologically using the ELISA test (P00301/04, Institut Pourquier, Montpellier, France). ELISA negative animals were tested for virus isolation from their monocyte-derived macrophage cultures [7] and by PCR [8] on DNA isolated from macrophage cultures. Only animals that were found negative by all the methods used were declared CAEV free.…”

Section: Animalsmentioning

confidence: 99%

Immortalized goat milk epithelial cell lines replicate CAEV at high level

et al. 2001

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-Primary milk epithelial cells were isolated from CAEV-uninfected goats and three cell lines designated TIGMEC-1, TIGMEC-2 and TIGMEC-3 were established. The three cell lines retained their morphological characteristics of epithelial cells and expressed specific epithelial cytokeratin marker as well as the immortalizing SV40 large T antigen. The kinetics of growth of TIGMEC1, TIG-MEC2 and TIGMEC3 cell lines showed a doubling time of 24-48 hours while the parental cell lines became senescent after the passage 6 in cell culture. Like the parental primary cells, the three cell lines were found to be highly sensitive to CAEV-pBSCA, an infectious molecular clone of CAEV-CO strain, and to a French isolate CAEV-3112. TIGMEC cell lines infected with CAEV-pBSCA became chronically infected producing high virus titers in absence of cytopathic effects. These cell lines may be useful for study of the possible physiological alterations in mammary epithelial cells infected with small ruminant lentiviruses and their consequences on milk quality. On an other hand, these cell lines can be used to study their role in virus transmission and pathogenesis. CAEV / replication / epithelial cell lineRésumé -Des cellules épithéliales du lait immortalisées répliquent le CAEV avec une grande efficacité. Des cellules épithéliales primaires isolées à partir de chèvres non infectées par le CAEV ont été immortalisées pour dériver 3 lignées appelées TIGMEC-1, TIGMEC-2 et TIGMEC-3. Les cellules des 3 lignées ont conservé leurs caractéristiques morphologiques de cellules épithéliales et expriment la cytokératine, un marqueur spécifique des cellules épithéliales et l'antigène immortalisant T du virus SV40. La courbe de croissance des lignées TIGMEC-1, TIGMEC-2 et TIGMEC-3 montre une multiplication des cellules toutes les 24 à 48 heures alors que les cellules parentales ne Vet. Res. 32 (2001) 429-440 429

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“…EAST et al, 1993 HENEINE, 1993;RIMSTAD et al, 1993;BARLOUGH et al, 1994;LEROUX et al, 1995;CHEBLOUNE et al, 1996;RUSSO et al, 1997) (SHAH et al, 2004;FEITOSA et al, 2010;HASEGAWA, 2010 VAN DER MOLEN, 1987;ROWE et al, 1991;LARA, 2002), contudo em rebanhos com alta taxa de infecção a soroprevalência pode ser bastante elevada entre animais jovens (EAST et al, 1987). (NARAYAN et al, 1984); interrupção do ciclo viral pelo processamento incompleto das proteínas de superfície (CHEBLOUNE et al, 1996); replicação de variantes antigênicos na presença de anticorpos neutralizantes e produção de interferon, que diminui o índice de replicação e favorece a persistência do estímulo antigênico McGUIRE 1982;NARAYAN et al, 1984;ZINK et al, 1987;NORTON, 1993;BERTONI et al, 1994 RIMSTAD et al, 1993). A produção de anticorpos e sua concentração no sangue de um animal infectado são influenciadas por situações de estresse, pela presença de outras doenças, condição alimentar e idade (FRANKE, 1998).…”

Section: à Coordenação De Aperfeiçoamento De Pessoal De Nível Superiounclassified

Section: Forma Clínica Nervosaunclassified

“…Nesses casos a PCR tem se apresentado como potencial alternativa na identificação de animais com sorologia negativa ou dúbia (RIMSTAD et al, 1993;CHEBLOUNE et al, 1996). Vários sistemas, com iniciadores derivados das sequências dos genes gag ou pol, foram desenvolvidos para detecção de DNA pró-viral ou RNA viral em leucócitos, células do leite, de lavado brônquio-alveolar do líquido sinovial (REDDY; SAPP; HENEINE, 1993;RIMSTAD et al, 1993;BARLOUGH et al, 1994;LEROUX et al, 1995;CHEBLOUNE et al, 1996;RUSSO et al, 1997;CASTRO, 1998). Como alternativa para aumentar a quantidade do produto amplificado e permitir sua visualização pela coloração com brometo de etídio, tem-se usado iniciadores internos à região inicialmente amplificada (BARLOUGH et al, 1994;LEROUX et al, 1995), pois geralmente os produtos amplificados só são detectados após hibridização com sonda específica do fragmento do gene que se deseja amplificar (REDDY; SAPP; HENEINE, 1993;RIMSTAD et al, 1993;BARLOUGH et al, 1994;LEROUX et al, 1995;CHEBLOUNE et al, 1996;RUSSO et al, 1997;CASTRO, 1998 TCID 50 /mL (Grupo Controle 2).…”

Section: Forma Clínica Nervosaunclassified

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Estudo sobre a transmissibilidade do vírus da artrite encefalite caprina através do sêmen e da placenta

Hasegawa¹

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Variations in Lentiviral Gene Expression in Monocyte-derived Macrophages from Naturally Infected Sheep

Cited by 45 publications

References 25 publications

In vitro infection of aortic endothelial cells by caprine arthritis encephalitis virus enhances in vitro transmigration of peripheral blood leukocytes and modulates their phenotypic expression

In vitro infection of aortic endothelial cells by caprine arthritis encephalitis virus enhances in vitro transmigration of peripheral blood leukocytes and modulates their phenotypic expression

Immortalized goat milk epithelial cell lines replicate CAEV at high level

Estudo sobre a transmissibilidade do vírus da artrite encefalite caprina através do sêmen e da placenta

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