Variations in morphology, physiology, and multiple bioactive constituents of Lonicerae Japonicae Flos under salt stress

Cai, Zhichen; Liu, Xunhong; Chen, Huan; Yang, Rong; Chen, Jiajia; Zou, Lisi; Wang, Chengcheng; Chen, Jiali; Tan, Mengxi; Mei, Yuqi; Wei, Lifang

doi:10.1038/s41598-021-83566-6

Cited by 22 publications

(15 citation statements)

References 50 publications

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“…In the case of H. cannabinus , salt stress enhanced the total antioxidant capacity, stimulating the production of some bioactive compounds but only for low-salinity conditions, and some compounds were not synthesized anymore for high salt stress, although new compounds were detected [ 18 ]. Similar results of stimulation under low-salt stress conditions were obtained in the case of Glycyrrhiza uralensis [ 19 ], while researchers found a strong dependence of the concentration of the bioactive compounds on the salinity for Lonicera japonica [ 20 ].…”

Section: Introductionsupporting

confidence: 66%

Salinity Stress Influences the Main Biochemical Parameters of Nepeta racemosa Lam.

Lungoci

Motrescu

Filipov

et al. 2023

Plants

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In this work, the effects of salt stress on Nepeta racemosa Lam. were studied to analyze the possibility of using it as a potential culture for salinity-affected soils. A total of nine concentrations of salts—NaCl (18, 39, and 60 mg/100 g soil), Na2SO4 (50, 85, and 120 mg/100 g soil), and a mixture (9 g NaCl + 25 g Na2SO4, 19 g NaCl + 43 g Na2SO4, and 30 g NaCl + 60 g Na2SO4/100 g soil)—simulated real salinity conditions. Environmental electron microscopy offered information about the size and distribution of glandular trichomes, which are very important structures that contain bioactive compounds. The chlorophyll pigments, polyphenols, flavonoids, and antioxidant activity were determined based on spectrophotometric protocols. The results have shown a different impact of salinity depending on the salt type, with an increase in bioactive compound concentrations in some cases. The highest polyphenol concentrations were obtained for Na2SO4 variants (47.05 and 46.48 mg GA/g dw for the highest salt concentration in the first and second year, respectively), while the highest flavonoid content was found for the salt mixtures (42.77 and 39.89 mg QE/g dw for the highest concentrations of salt in the first and, respectively, the second year), approximately 100% higher than control. From the Pearson analysis, strong correlations were found between chlorophyll pigments (up to 0.93), antioxidant activity and yield for the first harvest (up to 0.38), and antioxidant activity and flavonoid content for the second harvest (up to 0.95). The results indicate the possibility of growing the studied plants in salt-stress soils, obtaining higher concentrations of bioactive compounds.

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Section: Introductionsupporting

confidence: 66%

Salinity Stress Influences the Main Biochemical Parameters of Nepeta racemosa Lam.

Lungoci

Motrescu

Filipov

et al. 2023

Plants

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“…Nevertheless, there are differences in the mechanisms of tolerance to salt stress among different species of plants. Osmotic stress is a direct response of plants to salt stress, and plants can mitigate their incurred damage by regulating intracellular regulatory substances 24 , 25 . In addition, the generation and transport of biomass is also an important factor in assessing the degree of salt stress in plants 26 .…”

Section: Discussionmentioning

confidence: 99%

Physiological response and proteomics analysis of Reaumuria soongorica under salt stress

Yan

Chong

Ming

et al. 2022

Sci Rep

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Soil salinity can severely restrict plant growth. Yet Reaumuria soongorica can tolerate salinity well. However, large-scale proteomic studies of this plant’s response to salinity have yet to reported. Here, R. soongorica seedlings (4 months old) were used in an experiment where NaCl solutions simulated levels of soil salinity stress. The fresh weight, root/shoot ratio, leaf relative conductivity, proline content, and total leaf area of R. soongorica under CK (0 mM NaCl), low (200 mM NaCl), and high (500 mM NaCl) salt stress were determined. The results showed that the proline content of leaves was positively correlated with salt concentration. With greater salinity, the plant fresh weight, root/shoot ratio, and total leaf area increased initially but then decreased, and vice-versa for the relative electrical conductivity of leaves. Using iTRAQ proteomic sequencing, 47 177 136 differentially expressed proteins (DEPs) were identified in low-salt versus CK, high-salt versus control, and high-salt versus low-salt comparisons, respectively. A total of 72 DEPs were further screened from the comparison groupings, of which 34 DEPs increased and 38 DEPs decreased in abundance. These DEPs are mainly involved in translation, ribosomal structure, and biogenesis. Finally, 21 key DEPs (SCORE value ≥ 60 points) were identified as potential targets for salt tolerance of R. soongolica. By comparing the protein structure of treated versus CK leaves under salt stress, we revealed the key candidate genes underpinning R. soongolica’s salt tolerance ability. This works provides fresh insight into its physiological adaptation strategy and molecular regulatory network, and a molecular basis for enhancing its breeding, under salt stress conditions.

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“…Nevertheless, there are differences in the mechanisms of tolerance to salt stress among different species of plants. Osmotic stress is a direct response of plants to salt stress, and plants can mitigate their incurred damage by regulating intracellular regulatory substances 21,22 . In addition, the generation and transport of biomass is also an important factor in assessing the degree of salt stress in plants 23 .…”

Section: Discussionmentioning

confidence: 99%

Physiological Response and Proteomics Analysis of Reaumuria soongorica Under Salt Stress

Yan¹,

Chong²,

Zhao³

et al. 2021

Preprint

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Soil salinity can severely restrict plant growth. Yet Reaumuria soongorica can tolerate salinity well. However, large-scale proteomic studies of this plant’s salinity response have yet to reported. Here, R. soongorica seedlings (4 months old) were used in an experiment where NaCl solutions simulated levels of soil salinity stress. The fresh weight, root/shoot ratio, leaf relative conductivity, proline content, and total leaf area of R. soongorica under CK (0 mM NaCl), low (200 mM NaCl), and high (500 mM NaCl) salt stress were determined. The results showed that the proline content of leaves was negatively correlated with salt concentration. With greater salinity, the plant fresh weight, root/shoot ratio, and total leaf area increased initially but then decreased, and vice-versa for the relative electrical conductivity of leaves. Using iTRAQ proteomic sequencing, 47, 177, 136 differentially expressed proteins (DEPs) were identified in low-salt vs. CK, high-salt vs. control, and high-salt vs. low-salt comparisons, respectively. A total of 72 DEPs were further screened from the groups, of which, 34 DEPs increased and 38 DEPs decreased in abundance. These DEPs are mainly involved in translation, ribosomal structure, and biogenesis. Finally, 21 key DEPs (SCORE value ≥ 60 point) were identified as potential targets for salt tolerance of R. soongolica. By comparing the protein structure of treated vs. CK leaves under salt stress, we revealed the key candidate genes underpinning R. soongolica’s salt tolerance ability. This works provides fresh insight into its physiological adaptation strategy and molecular regulatory network, and a molecular basis enhancing breeding, under salt stress conditions.

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Variations in morphology, physiology, and multiple bioactive constituents of Lonicerae Japonicae Flos under salt stress

Cited by 22 publications

References 50 publications

Salinity Stress Influences the Main Biochemical Parameters of Nepeta racemosa Lam.

Salinity Stress Influences the Main Biochemical Parameters of Nepeta racemosa Lam.

Physiological response and proteomics analysis of Reaumuria soongorica under salt stress

Physiological Response and Proteomics Analysis of Reaumuria soongorica Under Salt Stress

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