Variations in the Enzyme Histochemistry of the Placenta

Curzen, Peter

doi:10.1111/j.1471-0528.1964.tb04297.x

Cited by 32 publications

(16 citation statements)

References 22 publications

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“…In majority of villi in preeclamptic placentae very strong PALP activity was seen on basal membrane, apical microvillous surface of syncytiotrophoblast, cytoplasm of syncytiotrophoblast, cytotrophoblast and connective tissue stroma. This observation is in accordance with previous workers like Mangal et al [8] and Curzen P [14] who observed strong PALP activity in syncytiotrophoblast of preeclamptic placentae as compared to control placentae. Dempsey et al [15] reported PALP activity in connective tissue stroma of preeclamptic placentae.…”

Section: Discussionsupporting

confidence: 83%

“…[13] In present study it was found that PALP activity was more intense in preeclamptic placentae as compared to control placentae. This finding was similar to Mangal et al, Jeacock et al, Curzen P and Dempsey et al [8,11,14,15] who found increased amount of PALP localisation in preeclamptic placentae as compared to control placentae. In present study majority of villi in control placentae showed PALP activity localized over the basal membrane of syncytiotrophoblast, moderate activity was seen on the microvillous surface and cytoplasm of syncytiotrophoblast.…”

Section: Discussionsupporting

confidence: 80%

“…This finding is in accordance to previous workers like Curzen P [26], Mangal et al [8] and Kapoor et al [27] Women with high maternal blood pressure had higher levels of serum alkaline phosphatase and increased intensity of PALP localization in placentae. Similar findings were reported by Mangal et al [8] and Curzen P. [14] Abnormally high serum alkaline phosphatase levels represent placental damage and failing placental function. [14,27,28] Placental ischemia, necrosis, damage of chorionic villi and infarction can increase the levels of PALP in maternal serum.…”

Section: Discussionsupporting

confidence: 80%

“…Similar findings were reported by Mangal et al [8] and Curzen P. [14] Abnormally high serum alkaline phosphatase levels represent placental damage and failing placental function. [14,27,28] Placental ischemia, necrosis, damage of chorionic villi and infarction can increase the levels of PALP in maternal serum. [4,8] PALP shows rising levels in maternal serum in second and third trimester of pregnancy.…”

Section: Discussionsupporting

confidence: 80%

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Histochemistry of placental alkaline phosphatase in preeclampsia

Shevade

Arole

Paranjape

et al. 2016

Int J of Biomed & Adv Res

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Objectives: Placental alkaline phosphatase (PALP) is synthesized in placenta and increases with gestational age. Alkaline phosphatase supports pregnancy and could play an essential role in nutrient supply and growth of the fetus. Preeclampsia is a systemic disorder which affects 5 to 7 percent of women worldwide and is a major cause for maternal and neonatal morbidity and mortality. As it has a major role in fetal growth, nutrition and defense mechanism study of alkaline phosphatase enzymatic activity becomes essential. Methods: 50 normal and 50 preeclamptic placentae were collected immediately after delivery from Department of Obstetrics and Gynecology. Placentae were obtained from known preeclamptic consenting cases who had no history of hypertension before pregnancy or during first 20 weeks of gestation, who had consistently recorded systolic and diastolic blood pressure of 140 / 90 mm of Hg or above and proteinuria ≥ 300mg / 24 hrs. Alkaline phosphatase activity was demonstrated by using modified Gomori's method. Results: Intensity of PALP localization was stronger in preeclamptic placentae as compared to normotensive placentae. Conclusion: Placental ischemia is evident in preeclampsia. Uteroplacental insufficiency leads to increased syncytial damage in preeclamptic placentae which may lead to abnormally high PALP activity and consequent increase in serum alkaline phosphatase.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionsupporting

confidence: 80%

Section: Discussionsupporting

confidence: 80%

Section: Discussionsupporting

confidence: 80%

See 2 more Smart Citations

Histochemistry of placental alkaline phosphatase in preeclampsia

Shevade

Arole

Paranjape

et al. 2016

Int J of Biomed & Adv Res

View full text Add to dashboard Cite

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“…The ontogeny of 5' nucleotidase specific activity appeared to parallel that of another placental membrane enzyme, alkaline phosphatase (12,30), with greater activity seen at term than in midterm tissue. As observed previously (43), highest specific activity was seen in the 100,000 x g fractions.…”

Section: Discussionmentioning

confidence: 91%

Ontogeny of Growth Factor Receptors in the Human Placenta

et al. 1982

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An ultrahistochemical study of the distribution of acid and alkaline phosphatases in placentae from normal and complicated pregnancies

Jones

Fox

1976

The Journal of Pathology

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The subcellular localisation of acid and alkaline phosphatase has been studied in the trophoblast of placentae from both normal and complicated pregnancies. In placentae from uncomplicated pregnancies the number of trophoblastic acid-phosphatase-containing organelles decreases progressively as gestation proceeds whilst alkaline-phosphatase activity, although abundant at term, could not be demonstrated during the early stages of pregnancy. The acid-phosphatase-containing organelles are of two types; one is a small round body which is probably a lysosome whilst the other is a multivesicular body. The alkaline phosphatase is distributed mainly on the syncytial microvilli and plasma-membrane. It is suggested that the marked lysosomal activity during early pregnancy is related to the architectural refashioning of the placenta during this period and that there are two phosphatase-linked transfer systems in the trophoblast, one dependent upon acid-phosphatase-containing multivesicular bodies and being utilised during early pregnancy and the other reliant upon alkaline phosphatase and dominating during the second half of gestation. In placentae from prolonged pregnancies there is a further decrease in trophoblastic acid phosphatase and, usually, a continuing increase in alkaline-phosphatase activity. In placentae from babies of low birth weight this trend is sometimes reversed and alkaline-phosphatase activity either disappears or its reaction product diffuses throughout the syncytium; this is usually accompanied by a marked increase in the number of acid-phosphatase-containing multivesicular bodies. Placentae from women with pre-eclampsia show no loss of alkaline-phosphatase activity but are characterised by an increased number of lysosomal bodies.

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Variations in the Enzyme Histochemistry of the Placenta

Cited by 32 publications

References 22 publications

Histochemistry of placental alkaline phosphatase in preeclampsia

Histochemistry of placental alkaline phosphatase in preeclampsia

Ontogeny of Growth Factor Receptors in the Human Placenta

An ultrahistochemical study of the distribution of acid and alkaline phosphatases in placentae from normal and complicated pregnancies

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