Variations of enzyme activities in the haemocytes of scallop Chlamys farreri after infection with the acute virus necrobiotic virus (AVNV)

Xing, Jing; Lin, Tze–Yi; Zhan, Wenbin

doi:10.1016/j.fsi.2008.09.008

Cited by 56 publications

(44 citation statements)

References 27 publications

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“…PO has been detected in the haemolymph of C. gigas (Hellio et al, 2007). Xing et al (2008) reported an increase of PO detection in the haemocytes of scallop Chlamys farreri after a challenge with the acute virus necrobiotic virus (AVNV).…”

Section: Expression Of Immune-related Genes In Oshv-1 Challenged Pacimentioning

confidence: 99%

Suppression substractive hybridisation (SSH) and real time PCR reveal differential gene expression in the Pacific cupped oyster, Crassostrea gigas, challenged with Ostreid herpesvirus 1

Renault

Faury

Barbosa-Solomieu

et al. 2011

Developmental & Comparative Immunology

144

132

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Virus-induced genes were identified using suppression subtractive hybridisation (SSH) from Pacific cupped oyster, Crassostrea gigas, haemocytes challenged by OsHV-1. A total of 304 clones from SSH forward library were sequenced. Among these sequences, some homologues corresponded to (i) immune related genes (macrophage express protein, IK cytokine, interferon-induced protein 44 or multicopper oxidase), (ii) apoptosis related genes (Bcl-2) and (iii) cell signalling and virus receptor genes (glypican). Molecular characterization and phylogenic analysis of 3 immune-related genes (macrophage expressed protein, multicopper oxidase and immunoglobulin domain cell adhesion molecule) were performed. Finally, quantitative PCR revealed significant changes in the expression of immune related genes (multicopper oxidase, macrophage expressed protein, myeloid differentiation factor 88 and interferon-induced protein 44) in oysters experimentally challenged with OsHV-1. These findings provide a first basis for studying the role of innate immunity in response to viruses in bivalves and identified genes may serve as markers of interest in breeding programs in order to obtain selected oysters presenting OsHV-1 resistance.

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Section: Expression Of Immune-related Genes In Oshv-1 Challenged Pacimentioning

confidence: 99%

Suppression substractive hybridisation (SSH) and real time PCR reveal differential gene expression in the Pacific cupped oyster, Crassostrea gigas, challenged with Ostreid herpesvirus 1

Renault

Faury

Barbosa-Solomieu

et al. 2011

Developmental & Comparative Immunology

144

132

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“…Since the 1980s, AVNV has caused disease events in summer of farmed Chlamys farreri in China, with mortality reaching w90 % within 5-8 days after first appearance (Fu et al, 2005). Studies investigating the physiological and immunological responses of Chlamys farreri infected with AVNV have been undertaken (Chen et al, 2011(Chen et al, , 2014Tang et al, 2010;Xing et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Antiviral immunity in marine molluscs

Green

Raftos

Speck

et al. 2015

Journal of General Virology

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Marine molluscs, like all living organisms, are constantly exposed to viruses and have evolved efficient antiviral defences. We review here recent developments in molluscan antiviral immunity against viruses belonging to the order Herpesvirales. Emerging results suggest an interferon-like response and autophagy are involved in the antiviral defence of bivalves to viral infection. Multifunctional plasma proteins from gastropods and bivalves have been identified to have broadspectrum antiviral activity against mammalian viruses. The antiviral defences present in molluscs can be enhanced by genetic selection, as shown by the presence of oyster strains specifically resistant to ostreid herpesvirus type 1. Whether varying amounts or different isoforms of these antiviral plasma proteins contributes to genetic resistance is worthy of further research. Other evolutionarily conserved antiviral mechanisms, such as RNA interference and apoptosis, still need further characterization.

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“…An excess of reactive oxygen species (ROS), such as hydrogen peroxide (H 2 O 2 ), not only kill invasion pathogens but also damage the body itself, such as converting unsaturated fatty acids, destroying the cell membrane structure, and causing DNA degradation [28]. MDA is a hazardous peroxide produced by peroxidation, and its content indicates the degree of oxidation.…”

Section: Effects Of Salinity On the Antioxidant Indexes Of P Aibuhitmentioning

confidence: 99%

Effect of Salinity on the Growth Performance, Body Composition, Antioxidant Indexes of Perinereis aibuhitensis and Total Nitrogen in the Substrate

Lv¹,

Nie²,

Yu³

et al. 2017

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In this study, the effects of different salinity levels (9,12,15,18,21,24,27,30, and 33) on the growth performance, body composition, antioxidant indexes of Perinereis aibuhitensis (initial average mass, 20.4 ± 0.3 mg) and total nitrogen in the substrate were investigated. The survival rate, specific growth rate, feed coefficient, and protein efficiency ratio under different salinity levels were measured. The results showed that the survival rate of P. aibuhitensis at the salinity level of 9 was significantly lower than that of P. aibuhitensis at other salinity levels (P < 0.05). However, the survival rate of P. aibuhitensis at other salinity levels was not significant (P > 0.05). On the basis of quadratic polynomial fitting of the relationship between salinity levels and the specific growth rate, feed coefficient, and protein efficiency ratio, it was concluded that 25.36 -25.9 is the most suitable salinity range for the growth performance of P. aibuhitensis. The main body composition (moisture, crude fat, crude protein, and ash content) was measured at different salinity levels. The results indicated that, with the increase in salinity, the moisture content of P. aibuhitensis decreased gradually; in contrast, the ash content increased gradually, as the salinity level increased. However, in the salinity range of 18 to 33, the difference in ash content was not significant (P > 0.05). Salinity had a significant influence on the crude protein content (P < 0.05), while it had no significant influence on crude body fat. Crude protein showed an increasing trend with the salinity increasing from 9 to 24, and decreased with the salinity increasing from 24 to 33. Antioxidant indexes such as superoxide dismutase, catalase, vo MDA content was as low as the antioxidant activity at the salinity level of 24; this means low in vivo contents of active oxygen free radicals and excellent growth performance. This conclusion is consistent with that for other growth indexes. The total nitrogen content of the substrate in which the P. aibuhitensis specimens were cultured for 60 days was higher than the total nitrogen in the soil. With an increase in salinity, the total nitrogen content first decreased and then increased, and the lowest value was observed at the salinity level of about 24.

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Variations of enzyme activities in the haemocytes of scallop Chlamys farreri after infection with the acute virus necrobiotic virus (AVNV)

Cited by 56 publications

References 27 publications

Suppression substractive hybridisation (SSH) and real time PCR reveal differential gene expression in the Pacific cupped oyster, Crassostrea gigas, challenged with Ostreid herpesvirus 1

Suppression substractive hybridisation (SSH) and real time PCR reveal differential gene expression in the Pacific cupped oyster, Crassostrea gigas, challenged with Ostreid herpesvirus 1

Antiviral immunity in marine molluscs

Effect of Salinity on the Growth Performance, Body Composition, Antioxidant Indexes of Perinereis aibuhitensis and Total Nitrogen in the Substrate

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