Variations of hydrogen production and microbial community with heavy metals during fermentative hydrogen production

Cho, Yoon-A; Lee, Tae-Jin

doi:10.1016/j.jiec.2011.02.036

Cited by 28 publications

(10 citation statements)

References 33 publications

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“…Nitrogen gas was used as a carrier gas at a flow rate of 30 mL/min and the operating temperatures of the injection port, oven, and detector were 80, 100, and 100 respectively. Volatile fatty acids (VFAs) were analyzed using a high performance liquid chromatograph (HPLC) system (GILSON, France) equipped with a UV detector operated at 210nm and a Supelcogel C-610H (130 mm × 7.8 mm ID) column [8]. Chemical oxygen demand (COD) and volatile suspended solid (VSS) concentration were determined according to the standard method procedures [13].…”

Section: Methodsmentioning

confidence: 99%

“…To analyze the characteristics of the hydrogen-producing microbial community, DNA was extracted from microorganisms using PowerSoil DNA Isolation Kit (MO BIO Laboratories, Inc.). 341 f and 518 primers were used to amplify the 16S rDNA followed by separation by denaturing gradient gel electrophoresis (DGGE) [8,9]. The gels were subjected to UV illumination and photographed.…”

Section: Pcr-dgge Proceduresmentioning

confidence: 99%

“…The amount of hydrogen production with various arsenite concentrations was calculated using the hydrogen production ration (  ) (Eq. ( 2)) [8].   was defined as the fraction of hydrogen produced over 85 h by arsenite dosed seed microflora compared to the control.…”

Section: Kinetic Modelingmentioning

confidence: 99%

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Effects of arsenite and variation of microbial community on continuous bio-hydrogen production from molasses using a sequence batch reactor (SBR)

William

Lee

2015

Environmental Engineering Research

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This study investigated the effects of various arsenite concentrations on bio-hydrogen production from molasses using a sequence batch reactor (SBR) operated in a series of three batch cycles. In the first batch cycle, hydrogen production was stimulated at arsenite concentrations lower than 2.0 mg/L, while inhibition occurred at arsenite concentration higher than 2.0 mg/L compared to the control. Hydrogen production decreased substantially during the second batch cycle, while no hydrogen was produced during the third batch cycle at all tested concentrations. The toxic density increased with respect to the increase in arsenite concentrations (6.0 > 1.6 > 1.0 > 0.5 mg/L) and operation cycles (third cycle > second cycle > first cycle). The presence of microorganisms such as Clostridium sp. MSTE9, Uncultured Dysgonomonas sp. clone MEC-4, Pseudomonas parafulva FS04, and Uncultured bacterium clone 584CL3e9 resulted in active stimulation of hydrogen production, however, it was unlikely that Enterobacter sp. sed221 was not related to hydrogen production. The tolerance of arsenite in hydrogen producing microorganisms decreased with the increase in induction time, which resulted in severing the inhibition of continuous hydrogen production.

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Section: Methodsmentioning

confidence: 99%

Section: Pcr-dgge Proceduresmentioning

confidence: 99%

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Effects of arsenite and variation of microbial community on continuous bio-hydrogen production from molasses using a sequence batch reactor (SBR)

William

Lee

2015

Environmental Engineering Research

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“…Eqs. (1)-(3) indicate acid-base buffer and acid-carbonate buffer equilibrium reactions where Alk À is alkalinity, HAlk is protonated alkalinity, CO 3 2À are carbonate ions, HCO 3 À are bicarbonate ions, H 2 CO 3 is carbonic acid and CO 2 is carbon dioxide gas [86][87][88][89].…”

Section: Phmentioning

confidence: 99%

A review on the effect of bio-electrodes on denitrification and organic matter removal processes in bio-electrochemical systems

Mook

Aroua

Chakrabarti

et al. 2013

Journal of Industrial and Engineering Chemistry

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“…Affiliation of denaturing gradient gel electrophoresisBand 7,9,10,13,14)이 아연이나 구리와 같 은 중금속이 함유된 시료에서는 거의 발현되지 않은 것으 로 보아 중금속이 수소생산에 관여하는 미생물의 활성을 저 해한 것으로 사료된다. 아연이나 구리가 함유된 시료에서 선명하게 나타나는 Enterococcus casseliflavus strain 91로 규명된 Band 1의 경우 비록 중금속이 함유되지 않은 음식 물쓰레기 발효시료에서 자주 출현하는 미생물은 아니지만 수소를 생산할 수 있는 미생물로 알려져 있다.…”

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Variations of Hydrogen Production in the Presence of Heavy Metals During Anaerobic Fermentation of Food Waste

Lee¹,

Lee²

2017

J Korean Soc Environ Eng

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In this study, variations of hydrogen production were investigated with food waste fermentation in the presence of heavy metals. Hydrogen production was 79.48 mL/g COD with fermentation of food waste. In the presence of 1 mg/L of zinc, the hydrogen production was decreased about 60%. When the copper is present, the production of hydrogen is severely inhibited, while the coexistence of copper with zinc relaxes the inhibition of copper and restores hydrogen production. Butyric acid or acetic acid was observed as the main species during hydrogen production. Klebsiella sp., Clostridium sp., and Dysgonomonas sp. were mainly appeared in the samples not containing heavy metals. However, Enterococcus sp. extremely influenced the hydrogen production activities of samples containing zinc or copper. 요약 : 본 연구에서는 음식물 쓰레기 혐기성 발효를 통한 수소생산에서 중금속이 미치는 영향에 대하여 살펴보았다. 음식물 쓰레기의 혐기성발효에 따른 수소생산량은 79.48 mL/g COD이였다. 1 mg/L의 아연이 함유되었을 때 약 60%의 수소생산량의 저해가 나타나는 것으로 나타났으며 구리가 아연보다 수소생산을 심각하게 저해하였다. 구리가 독립적으로 있을 때 수소생 산량이 심각하게 저해되는 것에 반하여 아연과 공존함으로서 구리의 저해작용이 완화되어 수소생산량이 회복되었다. 수소생 성과정 동안 butyric acid 또는 acetic acid가 주종으로 관찰되었으며 중금속이 함유되지 않은 시료에서는 Klebsiella sp., Clostridium sp., 그리고 Dysgonomonas sp.과 같은 군집이 출현하였으나 아연이나 구리가 함유되었을 경우 Enterococcus 종이 시료의 수소생산 활동에 큰 영향을 준 것으로 판단되었다. 주제어 : 음식물쓰레기, 혐기성 발효, 수소생산, 중금속

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Variations of hydrogen production and microbial community with heavy metals during fermentative hydrogen production

Cited by 28 publications

References 33 publications

Effects of arsenite and variation of microbial community on continuous bio-hydrogen production from molasses using a sequence batch reactor (SBR)

Effects of arsenite and variation of microbial community on continuous bio-hydrogen production from molasses using a sequence batch reactor (SBR)

A review on the effect of bio-electrodes on denitrification and organic matter removal processes in bio-electrochemical systems

Variations of Hydrogen Production in the Presence of Heavy Metals During Anaerobic Fermentation of Food Waste

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