Variations of Intracellular Ca<sup>2+</sup> Mobilization Initiated by Nanosecond and Microsecond Electrical Pulses in HeLa Cells

Ohnishi, Naofumi; Fujiwara, Y.; Kamezaki, Taichi; Katsuki, Sunao

doi:10.1109/tbme.2018.2886602

Cited by 8 publications

(5 citation statements)

References 34 publications

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“…Movies were recorded at 67 frames per second. The details of microscopy are provided in a previous paper [33].…”

Section: Methodsmentioning

confidence: 99%

Liposomes Under Real Cell Conditions Behave Like Real Cells with a Single Pulsed Electric Field

Urabe

Ogata

et al. 2019

Preprint

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15Liposomes are widely assumed to present a straightforward physical model of cells. 16However, almost all previous liposome experiments with pulsed electric fields (PEFs) 17 have been conducted in low-conductivity liquids, a condition that differs significantly 18 2 from that of cells in medium. Here, we prepared liposomes consisting of soy bean 19 lecithin and cholesterol, at a molar ratio of 1:1, in higher-conductivity liquid that 20 approximated the conditions of red blood cells in phosphate-buffered saline, with inner 21 and outer liquid conductivities of 0.6 and 1.6 S/m, respectively. We found that a single 22 1.1 kV/cm, 400 s PEF promoted cell-like spontaneous division of liposomes. 23 24

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“…Movies were recorded at 67 frames per second. The details of microscopy are provided in a previous paper [33].…”

Section: Methodsmentioning

confidence: 99%

Liposomes Under Real Cell Conditions Behave Like Real Cells with a Single Pulsed Electric Field

Urabe

Ogata

et al. 2019

Preprint

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“…1C). 19 Liposomes were exposed to one PEF at 400 ls duration with 76.40 V and 0.098 A, heating the sample theoretically at 2°C (Fig. 1D).…”

Section: Liposome Preparationmentioning

confidence: 99%

Pulsed Electric Fields Promote Liposome Buddings

Urabe

Ogata

et al. 2021

Bioelectricity

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Background: Liposomes have been a useful tool to analyze membrane behavior. Various studies have attempted to induce biological activities, for example, buddings, divisions, and endocytosis, on liposomes, focusing on lipid rafts that move along electric fields. Materials and Methods: Liposomes consisting of soybean lecithin, phosphatidylcholine, and cholesterol were prepared, with inner and outer liquid conductivities of 0.595 and 1.564 S/m, respectively. Results: We tried to induce buddings by pulsed electric fields (PEFs) on liposomes. Results demonstrated that 1.248 kV/cm, 400 ls PEF promoted postpulse liposome buddings, which were preceded by a membrane relaxation. Although a transient thick area (a lipid raft-like area) on the membrane just after PEF application preceded buddings, it was not the sufficient factor for buddings. Conclusion: We established a brief model as follows: 1.248 kV/cm, 400 ls PEF induced the lipid membrane relaxation without electroporation to trigger buddings. The current results could be a new frontier in bioelectrics.

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“…Additionally, a Correspondence to: Ippei Yagi. E-mail: yagi-3@tmu.ac.jp Department of Electrical Engineering and Computer Science, Graduate School of Systems Design, Tokyo Metropolitan University, 6-6, Asahigaoka, Hino-shi Tokyo, Japan 191-0065, an increase in intracellular Ca 2+ concentration and a decrease in Ψ m and an increase in reactive oxygen species immediately after nsPEF application have recently been reported using the technique of observing changes over time in fluorescence-stained cells [5,6].…”

Section: Introductionmentioning

confidence: 99%

“…It has also been shown that the decrease in mitochondrial membrane potential

{\Delta \varPsi}_{\mathrm{m}}

is caused by the release of

{\mathrm{Ca}}^{2+}

into the cytosol and that the frequency component in nsPEF corresponds to the shift in

{\Delta \varPsi}_{\mathrm{m}}

[1,4]. Additionally, an increase in intracellular

{\mathrm{Ca}}^{2+}

concentration and a decrease in

{\Delta \varPsi}_{\mathrm{m}}

and an increase in reactive oxygen species immediately after nsPEF application have recently been reported using the technique of observing changes over time in fluorescence‐stained cells [5,6].…”

Section: Introductionmentioning

confidence: 99%

Effects of Pulse Width and Electrical Energy of Low‐Voltage Nanosecond Pulsed Electric Fields on Mitochondria in Cancer Cells

Ninagawa,

Sugiura,

Kato

et al. 2024

IEEJ Transactions Elec Engng

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Nanosecond pulsed electric fields (nsPEFs) have recently shown promise as a cancer therapy. Although nsPEF‐induced apoptotic responses have been observed, the stress on individual cell components has not been quantified. Therefore, the authors built a cell equivalent circuit, including mitochondria, to quantitatively calculate the voltage, electric field, and electrical energy applied to the cell components in the frequency domain. Additionally, pulse width and electrical energy changed in mitochondrial membrane potential over time. The results indicated that the change of pulsed voltage switched the cell stimulation pathway, resulting in different mitochondrial membrane potentials over time. The frequency response analysis confirmed that the potential on mitochondrial membranes increased under shorter pulse width conditions. This suggested that the frequency analysis provided in this paper is useful for relating the site of cellular stimulation to induce physiological effects. © 2024 Institute of Electrical Engineer of Japan and Wiley Periodicals LLC.

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Variations of Intracellular Ca²⁺ Mobilization Initiated by Nanosecond and Microsecond Electrical Pulses in HeLa Cells

Cited by 8 publications

References 34 publications

Liposomes Under Real Cell Conditions Behave Like Real Cells with a Single Pulsed Electric Field

Liposomes Under Real Cell Conditions Behave Like Real Cells with a Single Pulsed Electric Field

Pulsed Electric Fields Promote Liposome Buddings

Effects of Pulse Width and Electrical Energy of Low‐Voltage Nanosecond Pulsed Electric Fields on Mitochondria in Cancer Cells

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Variations of Intracellular Ca2+ Mobilization Initiated by Nanosecond and Microsecond Electrical Pulses in HeLa Cells

Cited by 8 publications

References 34 publications

Liposomes Under Real Cell Conditions Behave Like Real Cells with a Single Pulsed Electric Field

Liposomes Under Real Cell Conditions Behave Like Real Cells with a Single Pulsed Electric Field

Pulsed Electric Fields Promote Liposome Buddings

Effects of Pulse Width and Electrical Energy of Low‐Voltage Nanosecond Pulsed Electric Fields on Mitochondria in Cancer Cells

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Variations of Intracellular Ca²⁺ Mobilization Initiated by Nanosecond and Microsecond Electrical Pulses in HeLa Cells