Varicella‐zoster virus in cerebrospinal fluid at relapses of multiple sclerosis

Sotelo, Julio; Martı́nez-Palomo, Adolfo; Ordóñez, Graciela; Pineda, Benjamín

doi:10.1002/ana.21316

Cited by 95 publications

(70 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In a previous study we have demonstrated that the Chomczynski RNA extraction, followed by a single-primer isothermal amplification, NGS, and metagenomic data analysis, enables to detect both DNA and RNA sequences (Perlejewski et al 2015). Interestingly, a relationship between VZV infection and demyelinating disorders has been previously suggested by the demonstration of more frequent presence of VZV-DNA and viral proteins in CSF of MS patients as compared to patients with other neurological diseases or healthy controls (Sotelo et al 2008;Mancuso et al 2007). Further, VZV-DNA is more prevalent in CSF and peripheral blood mononuclear cells during MS relapse than in remission (Sotelo et al 2014;Ordonez et al 2004).…”

Section: Discussionmentioning

confidence: 94%

Metagenomic Analysis of Cerebrospinal Fluid from Patients with Multiple Sclerosis

Perlejewski

Bukowska-Ośko

Nakamura

et al. 2016

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of central nervous system of unknown etiology. However, some infectious agents have been suggested to play a significant role in its pathogenesis. Next-generation sequencing (NGS) and metagenomics can be employed to characterize microbiome of MS patients and to identify potential causative pathogens. In this study, 12 patients with idiopathic inflammatory demyelinating disorders (IIDD) of the central nervous system were studied: one patient had clinically isolated syndrome, one patient had recurrent optic neuritis, and ten patients had multiple sclerosis (MS). In addition, there was one patient with other non-inflammatory neurological disease. Cerebrospinal fluid (CSF) was sampled from all patients. RNA was extracted from CSF and subjected to a single-primer isothermal amplification followed by NGS and comprehensive data analysis. Altogether 441,608,474 reads were obtained and mapped using blastn. In a CSF sample from the patient with clinically isolated syndrome, 11 varicella-zoster virus reads were found. Other than that similar bacterial, fungal, parasitic, and protozoan reads were identified in all samples, indicating a common presence of contamination in metagenomics. In conclusion, we identified varicella zoster virus sequences in one out of the 12 patients with IIDD, which suggests that this virus could be occasionally related to the MS pathogenesis. A widespread bacterial contamination seems inherent to NGS and complicates the interpretation of results.

show abstract

Section: Discussionmentioning

confidence: 94%

Metagenomic Analysis of Cerebrospinal Fluid from Patients with Multiple Sclerosis

Perlejewski

Bukowska-Ośko

Nakamura

et al. 2016

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

show abstract

“…Presence of VZV DNA, however, has been reported in blood cells [45] and CSF [46] from relapsing MS patients. Furthermore, Sotelo et al observed electron microscopically abundance of viral particles identical to VZV in the CSF of MS patients during the initial days of disease relapse [47]. However, when Burgoon et al used identical electron microscopic and PCR techniques they failed to identify presence of viral particles or DNA in MS CSF or acute MS plaques [48].…”

Section: Varicella-zoster Virusmentioning

confidence: 99%

Environmental triggers of multiple sclerosis

Kakalacheva

Lünemann

2011

FEBS Letters

View full text Add to dashboard Cite

a b s t r a c tMultiple sclerosis is a chronic immune-mediated disease of the central nervous system that develops in young adults with a complex genetic predisposition. Similar to other autoimmune disease, HLA-DR and -DQ alleles within the HLA class II region on chromosome 6p21 are by far the strongest risk-conferring genes. Less robust susceptibility effects have been reported for non-MHC related genetic variants. Improvements in the design of epidemiological studies helped to identify consistent environmental risk-associations such as the increased susceptibility for MS in individuals with a history of infectious mononucleosis, a symptomatic primary infection with the human c-herpesvirus Epstein-Barr virus (EBV). Sun exposure and serum vitamin D levels are emerging non-infectious environmental risk factors that may have independent roles. The analysis of environmental effects will likely expand in the next few years and will allow for the generation of testable hypotheses as to how environmental insults interact with genetic factors to jointly determine the susceptibility to MS. Insights gained from these studies might facilitate the development of prevention strategies and more effective treatments for MS.

show abstract

“…Varicella is usually benign in children; however, it may also result in complications that might include pneumonia, encephalitis, multiple sclerosis, and death (18,21,24). Primary infection is commonly followed by the establishment of lifelong latent infection of the sensory ganglia, which can be reactivated later in life, resulting in herpes zoster (shingles).…”

mentioning

confidence: 99%

Simultaneous Cocirculation of Both European Varicella-Zoster Virus Genotypes (E1 and E2) in Mexico City

et al. 2010

View full text Add to dashboard Cite

Full-length genome analysis of varicella-zoster virus (VZV) has shown that viral strains can be classified into seven different genotypes: European (E), Mosaic (M), and Japanese (J), and the E and M genotypes can be further subclassified into E1, E2, and M1 through 4, respectively. The distribution of the main VZV genotypes in Mexico was described earlier, demonstrating the predominance of E genotype, although other genotypes (M1 and M4) were also identified. However, no information regarding the circulation of either E genotype in the country is available. In the present study, we confirm the presence of both E1 and E2 genotypes in the country and explore the possibility of coinfection as the triggering factor for increased virulence among severe cases. A total of 61 different European VZV isolates collected in the Mexico City metropolitan area from 2005 to 2006 were typed by using a PCR method based on genotype-specific primer amplification. Fifty isolates belonged to the E1 genotype, and the eleven remaining samples were classified as E2 genotypes. No coinfection with both E genotypes was identified among these specimens. We provide here new information on the distribution of VZV genotypes circulating in Mexico City.

show abstract

Varicella‐zoster virus in cerebrospinal fluid at relapses of multiple sclerosis

Cited by 95 publications

References 39 publications

Metagenomic Analysis of Cerebrospinal Fluid from Patients with Multiple Sclerosis

Metagenomic Analysis of Cerebrospinal Fluid from Patients with Multiple Sclerosis

Environmental triggers of multiple sclerosis

Simultaneous Cocirculation of Both European Varicella-Zoster Virus Genotypes (E1 and E2) in Mexico City

Contact Info

Product

Resources

About