Varietal Characterization in Hazelnut by Rapd Markers

Miaja, M. L.; Vallania, R.; Me, C.; Akkak, A.; Nassi, O.; Lepori, G.

doi:10.17660/actahortic.2001.556.35

Cited by 6 publications

(5 citation statements)

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“…The genetic diversity of hazelnut cultivars was first assessed using randomly amplified polymorphic DNA (RAPD) (Galderisi et al 1999;Miaja et al 2001;Kafkas et al 2009) and amplified fragment length polymorphism (AFLP) (Ferrari et al 2004;Kafkas et al 2009) markers. AFLP was used to fingerprint 57 clones (Ferrari et al 2004) and, in combination with other markers, to assess diversity in 18 Turkish hazelnut cultivars (Kafkas et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Molecular genetic diversity and association mapping of nut and kernel traits in Slovenian hazelnut (Corylus avellana) germplasm

Öztürk

Çelik

et al. 2017

Tree Genetics & Genomes

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European hazelnut (Corylus avellana L.), cultivated in several areas of the world including Europe, Anatolia, and the USA, is an economically important nut crop due to its high mineral, oleic acid, amino acid, and phenolic compound content and pleasant flavor. This study examined molecular genetic diversity and population structure of 54 wild accessions and 48 cultivars from the Slovenian national hazelnut collection using amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers. Eleven AFLP primer combinations and 49 SSR markers yielded 532 and 504 polymorphic fragments, respectively. As expected for a wind-pollinated, self-incompatible species, levels of genetic diversity were high with cultivars and wild accessions having mean dissimilarity values of 0.50 and 0.60, respectively. In general, cultivars and wild accessions clustered separately in dendrogram, principal coordinate, and population structure analyses with regional clustering of the wild material. The accessions were also characterized for ten nut and seven kernel traits and some wild accessions were shown to have breeding potential. Morphological principal component analysis showed distinct clustering of cultivars and wild accessions.An association mapping panel composed of 64 hazelnut cultivars and wild accessions had considerable variation for the nut and kernel quality traits. Morphological and molecular data were associated to identify markers controlling the traits. In all, 49 SSR markers were significantly associated with nut and kernel traits [P < 0.0001 and LD value (r 2 ) = 0.15-0.50]. This work is the first use of association mapping in hazelnut and has identified molecular markers associated with important quality parameters in this important nut crop.

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Section: Introductionmentioning

confidence: 99%

Molecular genetic diversity and association mapping of nut and kernel traits in Slovenian hazelnut (Corylus avellana) germplasm

Öztürk

Çelik

et al. 2017

Tree Genetics & Genomes

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“…The traditional methods to characterize and identify cultivars and rootstocks in fruit tree species are based on phenotypic observations, but this approach is slow and subject to environmental influences mainly due to the long-time generation for trees. In hazelnut, molecular markers as random amplified polymorphic DNA (RAPD) and simple sequence repaet (SSR) were used in the cultivar fingerprint (Galderisi et al 1999;Miaja et al 2001;Ferrari et al 2004), identification of self-incompatibility alleles (Pomper et al 1998;Mehlenbacher 1997) resistance to eastern filbert blight (Mehlenbacher et al 2004;Chen et al 2005) and to analyze genetic relationships among cultivars Rovira Botta 2006, 2008;Gökirmak et al 2009). Amplified fragment length polymorphisms (AFLP) have also been used as a DNA fingerprinting technique to assess genome-wide diversity for individuals, populations and species, cultivars identification (Ferrari et al 2004;Kafkas et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Genetic relationship among wild, landraces and cultivars of hazelnut (Corylus avellana) from Portugal revealed through ISSR and AFLP markers

et al. 2013

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The genus Corylus, a member of the birch family Betulaceae, includes several species that are widely distributed throughout temperate regions of the Northern Hemisphere. This study assesses the genetic diversity in 26 international cultivars and 32 accessions of Corylus avellana L. from Portugal: 13 wild genotypes and 19 landraces. The genetic relationships among the 58 hazelnuts (Corylus avellana L.) were analyzed using inter simple sequence repeat (ISSR) and amplified fragment length polymorphism (AFLP) markers. Eighteen ISSR primers and seven AFLP primer pairs generated a total of 570 unambiguous and repeatable bands, respectively, from which 541 (95.03 %) were polymorphic for both markers. Genetic similarity index values ranged from 0.239 for wild types and cultivars to 0.143 for landraces and wild types. The genetic relationships were presented as a Neighbor-Joining method dendrogram and a two-dimensional principal coordinate analysis (PCoA) plot. The Neighbor-Joining dendrogram showed three main clusters, and the PCoA analysis has shown to be congruent with the hierarchical analysis. Bayesian analysis clustered all individuals into three groups showing a good separation among wild genotypes, landraces and cultivars. The genetic diversity found on wild genotypes and Portuguese landraces may provide relevant information for the diversity conservation and it will be useful in breeding programs and to identify local selections for preservation.

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“…1998;Bassil and Azarenko 2001), eastern filbert blight resistance (Mehlenbacher et al 2004a), genetic characterization (Radicati et al 1997;Galderisi et al 1999), and phylogenetic relations among important cultivars (Miaja et al 2001). Chloroplast microsatellite and PCR-RFLP markers have been used to describe the pattern of haplotypic variation and to investigate the postglacial migration history of C. avellana in Europe (Palmé and Vendramin 2002).…”

Section: Introductionmentioning

confidence: 99%

DNA typing and genetic relations among European hazelnut (Corylus avellanaL.) cultivars using microsatellite markers

Boccacci¹,

Akkak²,

Botta³

2006

Genome

104

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In this work, 78 hazelnut (Corylus avellana L.) cultivars from various germplasm repositories were studied at 16 simple sequence repeat (SSR) loci in order to identify the genotypes and investigate their genetic relations. Polymorphism at SSR loci was evaluated on the basis of number of alleles (mean: 9.4), expected heterozygosity (mean: 0.78), and power of discrimination (mean: 0.91). Several synonyms reported in the literature were confirmed, and new cases of synonymy were identified. The parentage of North American cultivars 'Butler', 'Ennis', and 'Royal', the French selection 'Fercoril-Corabel', and 'Impératrice Eugenie' was investigated on the basis of the alleles present at 16 loci and analysis at 8 additional loci. A dendrogram generated from cluster analysis using the unweighted pair group method with arithmetic mean grouped cultivars according to their pedigrees or geographical origins. There was an evident differentiation of the northern European cultivars from the southern European ones and from the Turkish cultivars. The latter clustered close to but separate from the Italian and Spanish clusters. It is very likely that exchanges of cultivars occurred between the central and western Mediterranean basin as a result of human migration and trade. A database containing the SSR profiles of the most important hazelnut cultivars will be useful for identification of cultivars and synonyms, legal protection, and parentage analysis.

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Varietal Characterization in Hazelnut by Rapd Markers

Cited by 6 publications

References 0 publications

Molecular genetic diversity and association mapping of nut and kernel traits in Slovenian hazelnut (Corylus avellana) germplasm

Molecular genetic diversity and association mapping of nut and kernel traits in Slovenian hazelnut (Corylus avellana) germplasm

Genetic relationship among wild, landraces and cultivars of hazelnut (Corylus avellana) from Portugal revealed through ISSR and AFLP markers

DNA typing and genetic relations among European hazelnut (Corylus avellanaL.) cultivars using microsatellite markers

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