“…The pulp was introduced into a tube containing 100 mg sodium metabisulphite, weighed and subsequently diluted (9:1, m/m) with 5 mol/L sulphuric acid (Rolle, Giacosa, Gerbi, Bertolino, & Novello, 2013;Rolle, Giacosa, Gerbi, & Novello, 2011;Río Segade, Giacosa, de Palma et al, 2013). Afterwards, the pulp was homogenised at 9500 rpm for 30 s with an Ultraturrax T10 high-speed homogenizer (IKA Labortechnik, Staufen, Germany) and centrifuged in a PK 131 centrifuge (ALC International, MI, Italy) for 15 min at 3000Âg at 20°C.…”