Varietal differences in terpene composition of blackcurrant (Ribes nigrum L) berries by solid phase microextraction/gas chromatography

Abstract

Relative amounts of volatile terpenes in berries of 10 different blackcurrant (Ribes nigrum L) cultivars were examined by solid phase microextraction/gas chromatography (SPME/GC). The optimisation of a variety of parameters affecting SPME enabled relative standard deviations from three replicates ranging from 2 to 12% to be achieved. Differences between cultivars in the proportions rather than in the qualitative composition of volatile terpenes were found, and the proportions of some terpenes were especially variable. Furthermore, the enantiomeric ratios of the chiral terpenes were determined for the first time in blackcurrant berries by GC using a column with a stationary phase containing permethylated cyclodextrin (Chirasil-b-Dex). The enantiomeric compositions of the majority of the chiral terpenes varied within a reasonably narrow range. However, the levels of two monoterpene alcohols, terpinen-4-ol and linalool, exhibited considerable variations amongst cultivars.

Natural variability of the enantiomeric composition of bioactive chiral terpenes in Mentha piperita

“…Although the obtained results allowed to determine reliably the terpenic composition of Mentha piperita, two different SPME-fibres (65 m layer of PDMS/DVB and 85 m layer of polyacrylate) were additionally tested under the selected temperature and time conditions in order to evaluate their performance. The choice of the last two mentioned fibres was based on our previous experience in the extraction of terpenes from different matrices, other than plants [18,19] and on the theoretical adequacy of polyacrylate fibre for terpenic compounds, respectively. However, neither of these fibres provided higher peak areas than those obtained when PDMS was utilised.…”

Odour-active components of simulated beef flavour analysed by solid phase microextraction and gas chromatography–mass spectrometry and –olfactometry

“…Generally, terpenoids originate from herbs or plants. For example, the unique intricate terpenic odour of blackcurrant berries was given oV from the terpene-containing glands in the plant (Ruiz del Castillo & Dobson, 2002). Mitiku, Ukeda, and Sawamura (2001) investigated the enantiomeric distribution in forty cold-pressed Citrus oil samples of -pinene, -pinene, sabinene and limonene, which are some of the same terpenoids found in our study, and reported that the species or varieties showed signiWcant diVerences in the enantiomeric purity of the terpenoids.…”

Changes in strawberry volatile constituents after pre‐harvest treatment with natural hormonal compounds

“…[22,36,38] This supports the known variability of the enantiomeric composition of monoterpenes in nature. [24,39] As observed in Table 3, the enantiomeric purity of (À)-DMHF, (À)-nerolidol and (+)-linalool in strawberries remained stable after pre-harvest treatments. From these results, it seems that natural hormonal compound treatments do not affect the enantioselective bioformation of (À)-DMHF, (À)-nerolidol and (+)-linalool during the ripening process.…”

“…However, the occurrence of pure (+)‐linalool in fruit aroma has previously been reported . This supports the known variability of the enantiomeric composition of monoterpenes in nature . As observed in Table , the enantiomeric purity of (−)‐DMHF, (−)‐nerolidol and (+)‐linalool in strawberries remained stable after pre‐harvest treatments.…”