Variety identification and comparative analysis of genetic diversity in yardlong bean (Vigna unguiculata spp. sesquipedalis) using morphological characters, SSR and ISSR analysis

Tantasawat, Piyada Alisha; Trongchuen, J.; Prajongjai, T.; Seehalak, Worapa; Jittayasothorn, Yingyos

doi:10.1016/j.scienta.2009.12.033

Cited by 62 publications

(49 citation statements)

References 53 publications

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“…In contrast, Asare et al (2010) reported 4 to 13 alleles in cowpea collected from �hana, while Sawadogo et al (2010) reported 5 to 12 alleles in cowpea collected from Burkina Faso using cross species SSRs from Medicago. These findings were in agreement with recent reports on the number of alleles detected using SSR makers in other legumes, such as, 14 to 67 alleles in chickpea (Upadhyaya et al, 2008), 9 to 14 in alfalfa (Mengoni et al, 2000), 1 to 9 in yardlong bean (Tantasawat et al, 2010), 11 to 26 in soybean (Rongwen et al, 1995) and 3 to 12 in pea (Sarikamis et al, 2010). The products amplified by primers SSR-6251 and SSR-6652 showed a high similarity with resistance gene protein analogs of Phaseolus vulgaris, Lens culinaris, Medicago truncatula, M. sativa, Pisum sativum suggesting a synteny between these species and cowpea.…”

Section: Discussionsupporting

confidence: 93%

Genetic relationship of cowpea (Vigna unguiculata) varieties from Senegal based on SSR markers

Badiane¹,

Gowda²,

Cissé³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Genetic diversity and phylogenetic relationships among 22 local cowpea (Vigna unguiculata) varieties and inbred lines collected throughout Senegal were evaluated using simple sequence repeat molecular markers. A set of 49 primer combinations were developed from cowpea genomic/expressed sequence tags and evaluated for their ability to detect polymorphisms among the various cowpea genotypes. Forty-four primer combinations detected polymorphisms, with the remaining five primer sets failing to yield PCR amplification products. From one to 16 alleles were found among the informative primer combinations; their frequencies ranged from 0.60 to 0.95 (mean = 0.79). The genetic diversity of the sample varied from 0.08 to 0.42 (mean = 0.28). The polymorphic information content ranged from 0.08 to 0.33 (mean = 0.23). The local varieties clustered in the same group, except 53-3, 58-53, and 58-57; while Ndoute yellow pods, Ndoute violet pods and Baye Ngagne were in the Genetic relationship of Vigna unguiculata second group. The photosensitive varieties (Ndoute yellow pods and Ndoute violet pods) were closely clustered in the second group and so were inbred line Mouride and local cultivar 58-57, which is also one of the parents for inbred line Mouride. These molecular markers could be used for selection and identification of elite varieties for cowpea improvement and germplasm management in Senegal.

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Section: Discussionsupporting

confidence: 93%

Genetic relationship of cowpea (Vigna unguiculata) varieties from Senegal based on SSR markers

Badiane¹,

Gowda²,

Cissé³

et al. 2012

Genet. Mol. Res.

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“…1), which proves the efficiency of the utilization of these loci for testing genotypic variability. The results of this study are in agreement with [22] that carried out genetic diversity analysis in 100 sorghum genotypes for drought tolerance using 13 stay-green specific polymorphic SSR markers [23]. The work revealed high level of polymorphism among the genotypes; there, about 56 scorable alleles were generated, of which 55 were polymorphic, and the number of alleles produced by different primers ranged from two to seven with an average of 4.0 alleles per primer.…”

Section: Ssr Analysissupporting

confidence: 89%

Field Performance of Five Soybean Mutants Under Drought Stress Conditions and Molecular Analysis Using SSR Markers

Yuliasti

Reflinur

2017

Atom Indo.

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The objectives of this research were to evaluate (1) the performance of soybean mutant lines under drought stress conditions, and (2) the genetic diversity and relationship among the mutant lines using SSR markers. The field evaluation was conducted during the dry season of 2011 and 2012 at the experimental Farm of Mataram University, West Nusa Tenggara, Indonesia. The field experiment was set up in a randomized block design. Ten mutant lines and two control varieties were evaluated in four replications. Genetic distance among evaluated lines were determined based on allelic diversity analysis using 40 simple sequence repeat (SSR) loci. Under drought stress conditions, two mutant lines, Kdl3 and Kdl8, showed a better performance compared to the other ones. The high yielding mutant lines were Kdl3 and Kdl8, which yielded 1.75 t ha-1 and 1.69 t ha-1, respectively, compared to the parent and national control, Panderman 1.43 t ha-1 and Muria 1.32 t ha-1. These mutant lines required 30.75 to 32 days to flower and 79.75 to 83.75 day to harvest with relatively short plant height 28.25 and 23.35 cm respectively. Those mutant characters were better than those of the other three mutants, the original parents, and the control soybean species. Since the evaluated soybean mutant lines yielded more under drought stress conditions than the standard varieties, they can be used and registered as drought-tolerant soybean mutants. Moreover, the evaluated soybean accessions showed a wide genetic distance. The accessions were clustered into two groups according to their genetic background, namely group I (the Panderman with three mutant lines) and group II (the Muria with two mutant lines). Twenty-three out of 40 evaluated SSR loci,

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“…Thus, molecular markers are tools that facilitate the study of various biological areas, such as cultivar identification, evolution and population genetics, and quantitative traits analysis in population genetics (Ritschel et al, 2004;Deleu et al, 2009;Fernández-Silva et al, 2009), with great opportunities for use in the genetic improvement of different crops, such as yardlong bean (Tantasawat et al, 2010), tomato (Mazzucato et al, 2010), and watermelon (Djé et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Genetic divergence among accessions of melon from traditional agriculture of the Brazilian Northeast

Aragão¹,

Filho²,

Nunes³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. The genetic divergence of 38 melon accessions from traditional agriculture of the Brazilian Northeast and three commercial hybrids were evaluated using fruit descriptors and microsatellite markers. The melon germplasm belongs to the botanic varieties cantalupensis (19), momordica (7), conomon (4), and inodorus (3), and to eight genotypes that were identified only at the species level. The fruit descriptors evaluated were: number of fruits per plant (NPF), fruit mass (FM; kg), fruit longitudinal diameter (LD; cm), fruit transversal diameter (TD; cm), shape index based on the LD/TD ratio, flesh pulp thickness, cavity thickness (CT; cm), firmness fruit pulp (N), and soluble solids (SS; °Brix). The results showed high variability for all Genetic diversity of Cucumis melo from Brazilian Northeast descriptors, especially for NPF, LD, and FM. The grouping analysis based on fruit descriptors produced eight groups without taxonomic criteria. The LD (22.52%), NPF (19.70%), CT (16.13%), and SS (9.57%) characteristics were the descriptors that contributed the most to genotype dissimilarity. The 17 simple sequence repeat polymorphic markers amplified 41 alleles with an average of 2.41 alleles and three genotypes per locus. Some markers presented a high frequency for the main allele. The genetic diversity ranged from 0.07 to 0.60, the observed heterozygosity had very low values, and the mean polymorphism information content was 0.32. Molecular genetic similarity analyses clustered the accessions in 13 groups, also not following taxonomic ranks. There was no association between morphoagronomic and molecular groupings. In conclusion, there was great variability among the accessions and among and within botanic groups.

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Variety identification and comparative analysis of genetic diversity in yardlong bean (Vigna unguiculata spp. sesquipedalis) using morphological characters, SSR and ISSR analysis

Cited by 62 publications

References 53 publications

Genetic relationship of cowpea (Vigna unguiculata) varieties from Senegal based on SSR markers

Genetic relationship of cowpea (Vigna unguiculata) varieties from Senegal based on SSR markers

Field Performance of Five Soybean Mutants Under Drought Stress Conditions and Molecular Analysis Using SSR Markers

Genetic divergence among accessions of melon from traditional agriculture of the Brazilian Northeast

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