Variimorphobacter saccharofermentans gen. nov., sp. nov., a new member of the family Lachnospiraceae, isolated from a maize-fed biogas fermenter

Abstract

Strain MD1T is an anaerobic, Gram-stain-negative bacterium isolated from a lab-scale biogas fermenter fed with maize silage. It has a rod-shaped morphology with peritrichously arranged appendages and forms long chains of cells and coccoid structures. The colonies of MD1T were white, circular, slightly convex and had a smooth rim. The isolate is mesophilic, displaying growth between 25 and 45 °C with an optimum at 40 °C. It grew at pH values of pH 6.7–8.2 (optimum, pH 7.1) and tolerated the addition of up to 1.5% (w/v) NaCl to the medium. The main cellular fatty acids of MD1T are C14:0 DMA and C16:0. Strain MD1T fermented xylose, arabinose, glucose, galactose, cellobiose, maltose, maltodextrin10, lactose starch, and xylan, producing mainly 2-propanol and acetic acid. The genome of the organism has a total length of 4163427 bp with a G+C content of 38.5 mol%. The two closest relatives to MD1T are Mobilitalea sibirica P3M-3T and Anaerotaenia torta FH052T with 96.44 or 95.8 % 16S rRNA gene sequence similarity and POCP values of 46.58 and 50.58%, respectively. As MD1T showed saccharolytic and xylanolytic properties, it may play an important role in the biogas fermentation process. Closely related variants of MD1T were also abundant in microbial communities involved in methanogenic fermentation. Based on morphological, phylogenetic and genomic data, the isolated strain can be considered as representing a novel genus in the family Lachnospiraceae , for which the name Variimorphobacter saccharofermentans gen. nov., sp. nov. (type strain MD1T=DSM 110715T=JCM 39125T) is proposed.

Effects of Artemisia ordosica Crude Polysaccharide on Antioxidant and Immunity Response, Nutrient Digestibility, Rumen Fermentation, and Microbiota in Cashmere Goats

“…According to research data, propionate has the ability to improve the conversion and storage of glucose, while butyrate provides energy to all tissues in the body [49]. The norank_f__norank_o__Bacteroidales and Saccharofermentans genera consume cellulose and hemicellulose, producing large amounts of propionate [50,51]. The norank_f__norank_o__Bacteroidales genus also showed a substantial positive correlation with propionate concentration, as well as a significant negative correlation with the acetate proportion and A/P ratio.…”

The epithelial transcriptome and mucosal microbiota are altered for goats fed with a low-protein diet

“…Meanwhile, the surge of H 2 S-producing Desulfobulbus in the mucosal microbiota during LP intervention (Cabrera et al, 2006 ) might also contribute to the disruption of immune stability in the ruminal mucosa. Intriguingly, LP inclusion declined the abundances of generally accepted carbohydrate-degrading bacteria in the ruminal mucosa, namely, fiber-degrading Fibrobacter and Ruminococcus (Yeoman et al, 2021 ), acetate producers Desulfovibrio and Saccharofermentans (Rettenmaier et al, 2021 ), succinate producers Succinimonas and Pyramidobacter (Gilbert et al, 2018 ), propionate producers Succiniclastium , and butyrate producers Butyrivibrio and Pseudobutyrivibrio (Pidcock et al, 2021 ). These highlighted the significance of ruminal microbiota in short-chain fatty acid (SCFA) production for energy supply and indicated a decline of carbohydrate fermentation in ruminal mucosa during LP intervention.…”

Co‐substrate composition is critical for enrichment of functional key species and for process efficiency during biogas production from cattle manure

“…Family Oscillospiraceae and Lachnospiraceae include members with saccharolytic capacity and with glycoside hydrolase enzymes responsible for the degradation of cellulose and hemicellulose (Beaumont et al, 2021 ; Laptev, 2021 ). Representatives of these families are common in rumen/gut environments, but have also been isolated from biogas environments (Flaiz et al, 2020 ; Rettenmaier et al, 2021 ). The availability of readily accessible carbohydrates in the form of soluble starch in R3 likely increased the relative abundance of the saccharolytic families, and potentially also increased their metabolic activity.…”