Various AKIP1 Expression Levels Affect Its Subcellular Localization but Have no Effect on NF-κB Activation

Keprová, Alena; Kořínková, L.; Křížová, Ivana; Hadravová, Romana; Kaufman, Filip; Pichová, Iva; Ruml, Tomáš; Rumlová, Michaela

doi:10.33549/physiolres.933961

Cited by 4 publications

(6 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A kinase-interacting protein 1, localizing in cytoplasm, nucleus, and mitochondria, functions as an adaptor of structural intracellular protein. 16 Recently, AKIP1 has been shown to facilitate tumorigenesis and invasiveness. [9][10][11][12] For example, one study displays that AKIP1 promotes cell migration, invasion, and EMT through mediating transactivating Zinc Finger E-Box Binding Homeobox 1 (ZEB1) in non-small-cell lung cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Retracted: A kinase‐interacting protein 1 may serve as a potential biomarker for deteriorative tumor features and poor prognosis in gastric cancer patients

Lin

Chen

et al. 2020

Clinical Laboratory Analysis

View full text Add to dashboard Cite

Objective This study aimed to explore the association of A kinase‐interacting protein 1 (AKIP1) expression with clinicopathological characteristics and prognosis in gastric cancer patients. Methods Data of 260 gastric cancer patients were retrospectively reviewed. AKIP1 expression in tumor tissue and non‐cancerous tissue specimens was detected by immunohistochemistry and semi‐quantitatively scored according to the staining intensity and density. Moreover, the clinicopathological features were retrieved, and disease‐free survival (DFS) and overall survival (OS) were calculated. Results A kinase‐interacting protein 1 expression was increased in tumor tissues compared with non‐cancerous tissues ( P < .001). In terms of tumor features, tumor AKIP1 high expression correlated with elevated T stage ( P < .001) and raised TNM stage ( P = .042), while did not correlate with pathological grade ( P > .999), tumor size ( P = .060), N stage ( P = .180), or tumor location ( P > .999). Meanwhile, tumor AKIP1 was not associated with the non‐tumor features either. Kaplan‐Meier curves disclosed that AKIP1 high expression patients had shorter DFS ( P = .004) and OS ( P = .043) compared with AKIP1 low expression patients. Univariate Cox's regression showed that AKIP1 high expression correlated with shorter DFS ( P = .005, hazard ratio [HR] = 1.635) and OS ( P = .046, HR = 1.519), whereas multivariate Cox's regression displayed that AKIP1 did not independently predict worse DFS ( P = .172, HR = 1.276) or shorter OS ( P = .433, HR = 1.183). Conclusion A kinase‐interacting protein 1 may serve as a potential biomarker for deteriorative tumor features and poor prognosis in gastric cancer patients.

show abstract

Section: Discussionmentioning

confidence: 99%

Retracted: A kinase‐interacting protein 1 may serve as a potential biomarker for deteriorative tumor features and poor prognosis in gastric cancer patients

Lin

Chen

et al. 2020

Clinical Laboratory Analysis

View full text Add to dashboard Cite

show abstract

“…Infectivity was determined similarly as described for Mason-Pfizer monkey virus [65][66][67][68][69][70][71]. Briefly, culture media from HEK 293 cells transfected with psPAX2, pWPXLd-GFP, and pHEF-VSV-G vectors in a 1:1:1 ratio in the presence of test compounds were collected 48 h post-transfection and filtered through a 0.45-µm filter.…”

Section: Single-round Infectivity Assaymentioning

confidence: 99%

“…The sample preparation and measurements were carried out as described elsewhere [68,70,71]. The VSV-G GFP-HIV infected cells were analyzed with a BD FACS Aria III flow cytometer (Becton Dickinson) with the excitation at 488 nm and the emission separated by a 530/30 band pass filter.…”

Section: Flow Cytometrymentioning

confidence: 99%

PF74 and Its Novel Derivatives Stabilize Hexameric Lattice of HIV-1 Mature-Like Particles

et al. 2020

View full text Add to dashboard Cite

A major structural retroviral protein, capsid protein (CA), is able to oligomerize into two different hexameric lattices, which makes this protein a key component for both the early and late stages of HIV-1 replication. During the late stage, the CA protein, as part of the Gag polyprotein precursor, facilitates protein–protein interactions that lead to the assembly of immature particles. Following protease activation and Gag polyprotein processing, CA also drives the assembly of the mature viral core. In the early stage of infection, the role of the CA protein is distinct. It controls the disassembly of the mature CA hexameric lattice i.e., uncoating, which is critical for the reverse transcription of the single-stranded RNA genome into double stranded DNA. These properties make CA a very attractive target for small molecule functioning as inhibitors of HIV-1 particle assembly and/or disassembly. Of these, inhibitors containing the PF74 scaffold have been extensively studied. In this study, we reported a series of modifications of the PF74 molecule and its characterization through a combination of biochemical and structural approaches. Our data supported the hypothesis that PF74 stabilizes the mature HIV-1 CA hexameric lattice. We identified derivatives with a higher in vitro stabilization activity in comparison to the original PF74 molecule.

show abstract

“…A-kinase interacting protein 1 (AKIP1), originally named breast cancer-associated protein 3, facilitates the nuclear translocation of catalytic subunit of protein kinase A (7,8). Recently, it has been reported that AKIP1 is viewed as a tumor promoter (7)(8)(9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

A-Kinase Interacting Protein 1 Knockdown Restores Chemosensitivity via Inactivating PI3K/AKT and β-Catenin Pathways in Anaplastic Thyroid Carcinoma

2022

View full text Add to dashboard Cite

BackgroundA-kinase interacting protein 1 (AKIP1) promotes tumor progression and chemoresistance in several malignancies; meanwhile, it is related to higher tumor size and recurrence risk of papillary thyroid carcinoma, while the role of AKIP1 in anaplastic thyroid carcinoma (ATC) is unclear. The aim of this study is to explore the effect of AKIP1 knockdown on cell malignant behaviors and doxorubicin resistance in ATC.MethodsAKIP1 knockdown was conducted in ATC cell lines (8505C and CAL-62 cells) by siRNA; then, cell viability, apoptosis, invasion, PI3K/AKT and β-catenin pathways, and doxorubicin sensitivity were detected. Subsequently, doxorubicin-resistant 8505C cells (8505C/Dox) were established. Additionally, AKIP1 was modified in 8505C and 8505C/Dox cells that underwent doxorubicin treatment by siRNA or overexpression plasmid, followed by cellular function and pathway detection.ResultsAKIP1 was elevated in FRO, 8505C, CAL-62, and KHM-5M cells compared to control cells (all p < 0.05). Subsequently, AKIP1 knockdown elevated apoptosis, inhibited viability and invasion, and inactivated PI3K/AKT and β-catenin pathways in 8505C and CAL-62 cells (all p < 0.05). AKIP1 knockdown decreased relative cell viability in doxorubicin-treated 8505C and CAL-62 cells; then, AKIP1 was elevated in 8505C/Dox cells compared to 8505C cells (all p < 0.05). Furthermore, AKIP1 knockdown restored doxorubicin sensitivity (reflected by decreased cell viability and invasion, and increased apoptosis), but inactivated PI3K/AKT and β-catenin pathways in doxorubicin-treated 8505C/Dox cells. However, AKIP1 overexpression presented an opposite effect on these functions and pathways in doxorubicin-treated 8505C cells.ConclusionAKIP1 knockdown decreases cell survival and invasion while promoting sensitivity to doxorubicin via inactivating PI3K/AKT and β-catenin pathways in ATC.

show abstract

Various AKIP1 Expression Levels Affect Its Subcellular Localization but Have no Effect on NF-κB Activation

Cited by 4 publications

References 24 publications

Retracted: A kinase‐interacting protein 1 may serve as a potential biomarker for deteriorative tumor features and poor prognosis in gastric cancer patients

Retracted: A kinase‐interacting protein 1 may serve as a potential biomarker for deteriorative tumor features and poor prognosis in gastric cancer patients

PF74 and Its Novel Derivatives Stabilize Hexameric Lattice of HIV-1 Mature-Like Particles

A-Kinase Interacting Protein 1 Knockdown Restores Chemosensitivity via Inactivating PI3K/AKT and β-Catenin Pathways in Anaplastic Thyroid Carcinoma

Contact Info

Product

Resources

About