Various methods of gold nanoparticles (GNPs) conjugation to antibodies

Jazayeri, Mir Hadi; Amani, Hamed; Pourfatollah, Ali Akbar; Pazoki‐Toroudi, Hamidreza; Sedighi-Moghaddam, B.

doi:10.1016/j.sbsr.2016.04.002

Cited by 437 publications

(315 citation statements)

References 61 publications

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“…A wide range of molecules, including adjuvants and antigens can be conjugated on AuNPs at high density, resulting in improved immunogenicity and antigen presentation (157,158). AuNPs can be formulated for intranasal administration and can diffuse into the lymph nodes, triggering robust antigenspecific cytotoxic T-cell immune responses (159,160).…”

Section: Inorganic Nanoparticlesmentioning

confidence: 99%

Nanoparticle-Based Vaccines Against Respiratory Viruses

et al. 2019

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The respiratory mucosa is the primary portal of entry for numerous viruses such as the respiratory syncytial virus, the influenza virus and the parainfluenza virus. These pathogens initially infect the upper respiratory tract and then reach the lower respiratory tract, leading to diseases. Vaccination is an affordable way to control the pathogenicity of viruses and constitutes the strategy of choice to fight against infections, including those leading to pulmonary diseases. Conventional vaccines based on live-attenuated pathogens present a risk of reversion to pathogenic virulence while inactivated pathogen vaccines often lead to a weak immune response. Subunit vaccines were developed to overcome these issues. However, these vaccines may suffer from a limited immunogenicity and, in most cases, the protection induced is only partial. A new generation of vaccines based on nanoparticles has shown great potential to address most of the limitations of conventional and subunit vaccines. This is due to recent advances in chemical and biological engineering, which allow the design of nanoparticles with a precise control over the size, shape, functionality and surface properties, leading to enhanced antigen presentation and strong immunogenicity. This short review provides an overview of the advantages associated with the use of nanoparticles as vaccine delivery platforms to immunize against respiratory viruses and highlights relevant examples demonstrating their potential as safe, effective and affordable vaccines.

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Section: Inorganic Nanoparticlesmentioning

confidence: 99%

Nanoparticle-Based Vaccines Against Respiratory Viruses

et al. 2019

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“…These binding reactions can be done by noncovalent, either hydrophobic or ionic interactions, or by covalent conjugations. [153] Noncovalent conjugations are particularly interesting due to their simplicity and spontaneity, without ligand degradation, where chemical reagents or catalysts are avoided. [154] However, this conjugation is less stable than the covalent one.…”

Section: Functionalized Poly(lactic-co-glycolic) Acid-based Drug Delimentioning

confidence: 99%

Functionalizing PLGA and PLGA Derivatives for Drug Delivery and Tissue Regeneration Applications

Martins

Sousa

Araújo

et al. 2017

Adv Healthcare Materials

213

133

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Poly(lactic‐co‐glycolic) acid (PLGA) is one of the most versatile biomedical polymers, already approved by regulatory authorities to be used in human research and clinics. Due to its valuable characteristics, PLGA can be tailored to acquire desirable features for control bioactive payload or scaffold matrix. Moreover, its chemical modification with other polymers or bioconjugation with molecules may render PLGA with functional properties that make it the Holy Grail among the synthetic polymers to be applied in the biomedical field. In this review, the physical–chemical properties of PLGA, its synthesis, degradation, and conjugation with other polymers or molecules are revised in detail, as well as its applications in drug delivery and regeneration fields. A particular focus is given to successful examples of products already on the market or at the late stages of trials, reinforcing the potential of this polymer in the biomedical field.

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“…Proteins (and particularly, immunoglobulins) spontaneously adhere to the surface of colloidal gold nanoparticles capped with citrate through several types of non-covalent interactions such as the attraction between hydrophobic parts of the protein and the metal surface and the electrostatic interactions between positively charged amino acids and N-terminus of the protein and the negatively charged surface of the citrate-capped particles [34].…”

Section: S-and -Dr-gnps Behavior During Conjugation With Antibodiesmentioning

confidence: 99%

“…The labeling of antibodies with GNPs for developing GNP-based ICST requires to define the minimum amount of the antibody to be adsorbed by nanoparticles in order to stabilize the nanoparticles themselves, which depends on the specific antibody used [7,[34][35].…”

Section: S-and -Dr-gnps Behavior During Conjugation With Antibodiesmentioning

confidence: 99%

Multicolor immunochromatographic strip test based on gold nanoparticles for the determination of aflatoxin B1 and fumonisins

et al. 2017

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Graphical abstractBlue desert rose-like gold nanoparticles (DR-GNPs) were synthesized, characterized and applied as label for the ImmunoChromatographic Strip Test (ICST) technique, in which red spherical GNPs (sGNPs) are usually employed. The combined use of the blue DR-GNP and red s-GNPs allowed developing of an intuitive multicolor ICST for the simultaneous detection of Aflatoxin B1 and AbstractDesert rose-like gold nanoparticles (DR-GNPs) that exhibited a blue color due to the plasmon resonance band at 620 nm were prepared. The blue DR-GNPs were obtained through a seeding growth approach and characterized by UV-vis spectroscopy, transmission electron microscopy and dynamic light scattering. The DR-GNPs had a hydrodynamic diameter of ~72 nm and were used as labels for the antibody in an immunochromatographic strip test (ICST). Although the particular shape of DR-GNPs and their higher surface area with respect to spherical GNPs, they demonstrated to be effective as antibody labels. A multicolor ICST for aflatoxin B1 and fumonisins was developed that employs both blue DR-and red spherical GNPs. The multicolor ICST allows for simultaneous rapid determination of the two mycotoxins in maize flour, with visual cut-off levels at 2 µg kg -1 for aflatoxin B1 and 1000 µg kg -1 for fumonisins.

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Various methods of gold nanoparticles (GNPs) conjugation to antibodies

Cited by 437 publications

References 61 publications

Nanoparticle-Based Vaccines Against Respiratory Viruses

Nanoparticle-Based Vaccines Against Respiratory Viruses

Functionalizing PLGA and PLGA Derivatives for Drug Delivery and Tissue Regeneration Applications

Multicolor immunochromatographic strip test based on gold nanoparticles for the determination of aflatoxin B1 and fumonisins

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