Various techniques for oocyte recovery in an in vitro fertilization and embryo transfer program

Belaïsch-Allart, J.; Hazout, A.; Guillet-Rosso, F.; Glissant, M.; Testart, J; Frydman, René

doi:10.1007/bf01139341

Cited by 33 publications

(10 citation statements)

References 13 publications

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“…[ 5 ] Transvaginal oocyte recovery is the least invasive, least painful, most accurate, and simple method to collect oocytes. [ 9 10 ] Apart from the inherent characteristics of the oocyte, its quality is affected by the techniques used for oocyte retrieval. The aspirating pressure used for oocyte retrieval can affect the integrity of the oocytes.…”

Section: Discussionmentioning

confidence: 99%

Oocyte retrieval at 140-mmHg negative aspiration pressure: A promising alternative to flushing and aspiration in assisted reproduction in women with low ovarian reserve

et al. 2015

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BACKGROUND:Oocyte retrieval in women with low ovarian reserve is challenging and often results in poor pregnancy outcomes.AIM:The aim was to compare outcomes of oocyte retrieval using three aspiration methods in women with normal and low antral follicle counts (AFCs).STUDY DESIGN:Retrospective cohort study.SETTING:Tertiary care hospital.MATERIALS AND METHODS:Data from 172 women who underwent in-vitro fertilization/intracytoplasmic sperm injection over 3 years was studied after dividing into three groups: Group A (96) - normal AFC (≥10), direct oocyte retrieval at 120 mmHg, Group B (41) - low AFC (<10), direct retrieval at 140-mmHg, Group C (35) - low AFC, follicular flushing, and aspiration at 120-mmHg. In each group, oocyte yield, embryo quality, and pregnancy out comes were assessed.RESULTS:The oocyte and embryo yield in Groups A and B were comparable (P > 0.05) but were significantly less in Group C (P < 0.05). The pregnancy rates in Groups A and B were similar (35.4%, 39%) but were significantly less (17.1%) in Group C (P < 0.05). The live birth rate was 76% in Group A, 69% in Group B, and 50% in Group C. The miscarriage rates in the three groups were 21%, 31%, and 50%, respectively.CONCLUSION:Direct oocyte retrieval with 140-mmHg showed significantly better oocyte yield and pregnancy outcomes compared to flushing and aspiration in women with low AFC.

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Section: Discussionmentioning

confidence: 99%

Oocyte retrieval at 140-mmHg negative aspiration pressure: A promising alternative to flushing and aspiration in assisted reproduction in women with low ovarian reserve

et al. 2015

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“…This was followed by hMG (two ampoules/day; Neopergonal; Serono, Levallois-Perret, France) on days 6, 8 and 10 of the cycle. The hMG treatment was continued until completion of follicular maturation, as estimated by hormone levels and ultrasonographic examinations (Belaisch-Allart, Hazout, Guillet-Rosso et al 1985). As soon as a concentration of 1-1-1-5 nmol oestradiol/1 for each follicle of more than 15 mm was reached, hCG (5000 i.u.…”

Section: Endometrial Biopsiesmentioning

confidence: 99%

Scanning electron microscopy of postovulatory human endometrium in spontaneous cycles and cycles stimulated by hormone treatment

Martel

Frydman²,

Glissant³

et al. 1987

Journal of Endocrinology

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105

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The ultrastructure of the luminal surface epithelium was compared in endometrial samples taken from 23 normally cycling women and from 22 patients submitted to ovarian stimulation with clomiphene citrate (100 mg/day for 5 days), human menopausal gonadotrophin (hMG) and human chorionic gonadotrophin (hCG). On day 2 after ovulation, only four out of nine specimens taken from the women in the hormone-treated group were identical to those of normally cycling women. On day 6 after ovulation, only two out of the 13 biopsy specimens from the treated group were the same as those from normally cycling women at that stage. Apical protrusions (pinopodes), typical for this period of the cycle, were missing in 11 of the 13 endometrial samples from the treated group. These observations suggest that the hormonal treatment applied to induce ovulation (clomiphene citrate, hMG and hCG) can modify the normal development of the prenidatory endometrium, and may thus have a negative effect on the rate of egg implantation.

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“…Higher percentages of blastocysts are obtained via in vivo maturation of oocytes compare to in vitro maturation (IVM). This kind of evidence suggests that in vitro milieu is not sufficient for supporting development and early embryo competence ( 5 ). Due to the significant impact of the seasons on ovine reproductive cycle, it is not uncommon for IVM-IVF systems to endure some deficiencies in non-breeding seasons ( 6 ).…”

Section: Introductionmentioning

confidence: 99%

In Vitro Ovine Embryo Production: the Study of Seasonal and Oocyte Recovery Method Effects

Davachi

Shahneh

Kohram

et al. 2014

Iran Red Crescent Med J

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Background:To current knowledge, different oocyte's recovery method and various seasons have profound impact on in vitro embryo production (IVEP).Objectives:The aim of this study was to define an efficient recovery method for oocytes harvesting from slaughterhouse material in different seasons, and their effects on IVEP yield.Materials and Methods:Ovaries from slaughtered ewes in breeding season (BS) and non-breeding season (NBS) were collected from a local abattoir. The oocytes were recovered through aspiration, centrifugation (ORC), puncture and slicing, and categorized into three classes (I, oocytes with more than three layers of cumulus cells; II, less than three layers with damaged cumulus cells; III, denuded oocytes). After cultivation in TCM 199 for 24 hours, matured oocytes were subjected to in vitro fertilization (IVF) and in vitro culture (IVC). The oocyte recovery using ORC in BS and NBS was significantly higher (P < 0.05) compared with other recovery methods.Results:No significant dissimilarities in the proportion of oocytes reaching M-II stage were recorded when using different oocyte recovery methods in different seasons. Aspiration resulted in lower (P < 0.05) proportion of class I (BS, 60.0 ± 2.1; NBS, 51.1 ± 2.1) compared to ORC (BS, 82.0 ± 1.2; NBS, 70.0 ± 1.2), slicing (BS, 80.0 ± 2.1; NBS, 71.0 ± 1.4) and puncture (BS, 80.0 ± 1.5; NBS, 72.0 ± 2.0). Monospermy and blastocyst development rates were significantly higher using ORC than other recovery techniques in both BS and NBS. More oocytes with high quality, greater blastocyst development and oocyte recovery rates were achieved in BS.Conclusions:The results revealed that oocytes harvesting technique and season are effective in the rate of cleavage and blastocysts’ development, and suggest that despite same meiotic resumption rate in all treatments, it would be better to use ORC.

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Various techniques for oocyte recovery in an in vitro fertilization and embryo transfer program

Cited by 33 publications

References 13 publications

Oocyte retrieval at 140-mmHg negative aspiration pressure: A promising alternative to flushing and aspiration in assisted reproduction in women with low ovarian reserve

Oocyte retrieval at 140-mmHg negative aspiration pressure: A promising alternative to flushing and aspiration in assisted reproduction in women with low ovarian reserve

Scanning electron microscopy of postovulatory human endometrium in spontaneous cycles and cycles stimulated by hormone treatment

In Vitro Ovine Embryo Production: the Study of Seasonal and Oocyte Recovery Method Effects

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