Varying Oxygen Partial Pressure Elicits Blood-Borne Microparticles Expressing Different Cell-Specific Proteins—Toward a Targeted Use of Oxygen?

Balestra, Costantino; Arya, Awadhesh K; Leveque, Clément; Virgili, Fabio; Germonpré, Peter; Lambrechts, Kate; Lafère, Pierre; Thom, Stephen R.

doi:10.3390/ijms23147888

Cited by 23 publications

(33 citation statements)

References 69 publications

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“…Other recent data on platelet (CD41a)-, astrocyte (TMEM119)-, and neutrophil (CD66+)related microparticle release after oxygen exposure (30% and 100%) [15] are consistent with this analysis.…”

Section: Discussionsupporting

confidence: 90%

“…This is further confirmed by microparticle expression under different oxygen partial pressures. All tested exposures demonstrated significant elevations of CD41, CD66b, TMEM, and expression and phalloidin binding, except for 1.4 ATA, which elicited the total opposite response with significant decreases of all measured parameters [15].…”

Section: Discussionmentioning

confidence: 90%

“…New research paths have coined the use of several inhaled oxygen fractions (FiO 2 ) in the physiology of aging [4,5], such as cartilage degeneration [6], prediabetes [7], or neuroprotection [8]. It must be acknowledged that variable FiO 2 , hypoxic or hyperoxic, is already relatively common, for instance, for preconditioning in cardiovascular disease [9,10], sports training [11], and before exposure to challenging environments such as scuba diving [12], high altitude military parachute freefall [13,14], and space flight activities [15].…”

Section: Introductionmentioning

confidence: 99%

“…Based on the current literature, several factors such as FiO 2 [11,15,24,25], duration and frequency [26][27][28], or pressure of exposure-either normobaric, hypobaric, or hyperbaric [15,26,29]-may influence the ability to reach a specific clinical or molecular effect.…”

Section: Introductionmentioning

confidence: 99%

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Oxidative Stress Response’s Kinetics after 60 Minutes at Different (30% or 100%) Normobaric Hyperoxia Exposures

Leveque

Mrakic‐Sposta

Lafère

et al. 2022

IJMS

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Oxygen is a powerful trigger for cellular reactions and is used in many pathologies, including oxidative stress. However, the effects of oxygen over time and at different partial pressures remain poorly understood. In this study, the metabolic responses of normobaric oxygen intake for 1 h to mild (30%) and high (100%) inspired fractions were investigated. Fourteen healthy non-smoking subjects (7 males and 7 females; age: 29.9 ± 11.1 years, height: 168.2 ± 9.37 cm; weight: 64.4 ± 12.3 kg; BMI: 22.7 ± 4.1) were randomly assigned in the two groups. Blood samples were taken before the intake at 30 min, 2 h, 8 h, 24 h, and 48 h after the single oxygen exposure. The level of oxidation was evaluated by the rate of reactive oxygen species (ROS) and the levels of isoprostane. Antioxidant reactions were observed by total antioxidant capacity (TAC), superoxide dismutase (SOD), and catalase (CAT). The inflammatory response was measured using interleukin-6 (IL-6), neopterin, creatinine, and urates. Oxidation markers increased from 30 min on to reach a peak at 8 h. From 8 h post intake, the markers of inflammation took over, and more significantly with 100% than with 30%. This study suggests a biphasic response over time characterized by an initial “permissive oxidation” followed by increased inflammation. The antioxidant protection system seems not to be the leading actor in the first place. The kinetics of enzymatic reactions need to be better studied to establish therapeutic, training, or rehabilitation protocols aiming at a more targeted use of oxygen.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Oxidative Stress Response’s Kinetics after 60 Minutes at Different (30% or 100%) Normobaric Hyperoxia Exposures

Leveque

Mrakic‐Sposta

Lafère

et al. 2022

IJMS

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“…New insight demonstrate that bubbles tear the vessel inner layer away and create microparticles of endothelial debris when detaching from the endothelium during decompression [ 8 , 9 , 10 ]. Bubbles and oxygen partial pressure increase trigger defense mechanisms like platelets and neutrophil activation that will also elicit some microparticles [ 11 , 12 ]. In this study, the vascular function assessed by means of Flow Mediated Dilation (FMD) was considered as the third dimension representing the oxidative and or inflammatory stress [ 10 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Vascular Function Recovery Following Saturation Diving

2022

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Background and Objectives: Saturation diving is a technique used in commercial diving. Decompression sickness (DCS) was the main concern of saturation safety, but procedures have evolved over the last 50 years and DCS has become a rare event. New needs have evolved to evaluate the diving and decompression stress to improve the flexibility of the operations (minimum interval between dives, optimal oxygen levels, etc.). We monitored this stress in saturation divers during actual operations. Materials and Methods: The monitoring included the detection of vascular gas emboli (VGE) and the changes in the vascular function measured by flow mediated dilatation (FMD) after final decompression to surface. Monitoring was performed onboard a diving support vessel operating in the North Sea at typical storage depths of 120 and 136 msw. A total of 49 divers signed an informed consent form and participated to the study. Data were collected on divers at surface, before the saturation and during the 9 h following the end of the final decompression. Results: VGE were detected in three divers at very low levels (insignificant), confirming the improvements achieved on saturation decompression procedures. As expected, the FMD showed an impairment of vascular function immediately at the end of the saturation in all divers but the divers fully recovered from these vascular changes in the next 9 following hours, regardless of the initial decompression starting depth. Conclusion: These changes suggest an oxidative/inflammatory dimension to the diving/decompression stress during saturation that will require further monitoring investigations even if the vascular impairement is found to recover fast.

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Exhaled breath condensate profiles of U.S. Navy divers following prolonged hyperbaric oxygen (HBO) and nitrogen-oxygen (Nitrox) chamber exposures

et al. 2023

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Prolonged exposure to hyperbaric hyperoxia can lead to pulmonary oxygen toxicity (PO2tox). PO2tox is a mission limiting factor for special operations forces divers using closed-circuit rebreathing apparatus and a potential side effect for patients undergoing hyperbaric oxygen (HBO) treatment. In this study, we aim to determine if there is a specific breath profile of compounds in exhaled breath condensate (EBC) that is indicative of the early stages of pulmonary hyperoxic stress/PO2tox. Using a double-blind, randomized “sham” controlled, cross-over design 14 U.S. Navy trained diver volunteers breathed two different gas mixtures at an ambient pressure of 2 ATA (33 fsw, 10 msw) for 6.5 hours. One test gas consisted of 100% O2 (HBO) and the other was a gas mixture containing 30.6% O2 with the balance N2 (Nitrox). The high O2 stress dive (HBO) and low O2 stress dive (nitrox) were separated by at least seven days and were conducted dry and at rest inside a hyperbaric chamber. EBC samples were taken immediately before and after each dive and subsequently underwent a targeted and untargeted metabolomics analysis using liquid chromatography coupled to mass spectrometry (LC-MS). Following the HBO dive, 10 out of 14 subjects reported symptoms of the early stages of PO2tox and one subject terminated the dive early due to severe symptoms of PO2tox. No symptoms of PO2tox were reported following the nitrox dive. A Partial Least-Squares Discriminant Analysis of the normalized (relative to pre-dive) untargeted data gave good classification abilities between the HBO and nitrox EBC with an AUC of 0.99 (± 2%) and sensitivity and specificity of 0.93 (± 10%) and 0.94 (± 10%), respectively. The resulting classifications identified specific biomarkers that included human metabolites and lipids and their derivatives from different metabolic pathways that may explain metabolomic changes resulting from prolonged HBO exposure.

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Varying Oxygen Partial Pressure Elicits Blood-Borne Microparticles Expressing Different Cell-Specific Proteins—Toward a Targeted Use of Oxygen?

Cited by 23 publications

References 69 publications

Oxidative Stress Response’s Kinetics after 60 Minutes at Different (30% or 100%) Normobaric Hyperoxia Exposures

Oxidative Stress Response’s Kinetics after 60 Minutes at Different (30% or 100%) Normobaric Hyperoxia Exposures

Vascular Function Recovery Following Saturation Diving

Exhaled breath condensate profiles of U.S. Navy divers following prolonged hyperbaric oxygen (HBO) and nitrogen-oxygen (Nitrox) chamber exposures

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