Varying the diameter of aligned electrospun fibers alters neurite outgrowth and Schwann cell migration

Wang, Han Bing; Mullins, Michael E.; Cregg, Jared M.; McCarthy, Connor W.; Gilbert, Ryan J.

doi:10.1016/j.actbio.2010.02.020

Cited by 276 publications

(309 citation statements)

References 33 publications

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“…The process is highly customizable and the fibers can be spun in nm or µm scales (Pettikiriarachchi et al, 2010). Polylactic acid (PLLA) fibers of large diameter (>1,000 nm) have been shown to enhance neurite extension in dissociated chick dorsal root ganglia (DRG) cultures (Wang et al, 2010). Functionalizing PLLA fibers with fibronectin or laminin further improves neurite interaction by replicating these endogenous chemical cues (Koppes et al, 2014).…”

Section: Topographical Cues Drive Alignment and Directionalitymentioning

confidence: 99%

Biomaterials for Enhancing Neuronal Repair

Cangellaris

Gillette

2018

Front. Mater.

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As they differentiate from neuroblasts, nascent neurons become highly polarized and elongate. Neurons extend and elaborate fine and fragile cellular extensions that form circuits enabling long-distance communication and signal integration within the body. While other organ systems are developing, projections of differentiating neurons find paths to distant targets. Subsequent post-developmental neuronal damage is catastrophic because the cues for reinnervation are no longer active. Advances in biomaterials are enabling fabrication of micro-environments that encourage neuronal regrowth and restoration of function by recreating these developmental cues. This mini-review considers new materials that employ topographical, chemical, electrical, and/or mechanical cues for use in neuronal repair. Manipulating and integrating these elements in different combinations will generate new technologies to enhance neural repair.

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Section: Topographical Cues Drive Alignment and Directionalitymentioning

confidence: 99%

Biomaterials for Enhancing Neuronal Repair

Cangellaris

Gillette

2018

Front. Mater.

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“…Then, PLLA is modified or copolymerized with other degradable polymers to reduce degradation time, as shown by the use of radiations to create radicals in the ester alpha carbon which, upon rearrangement, shortens the polymer backbone through the removal of an ester bond and the release of carbon dioxide [100,101]. PLLA is used as bone fixator, scaffold for bone [102,103], cartilage [104], tendon [105], neural [106], and vascular [107] regeneration. Similarly, PDLLA is an amorphous polymer with the random positions of its two isomeric monomers within the polymer chain.…”

Section: Polyestersmentioning

confidence: 99%

Biodegradable polymers for dental tissue engineering and regeneration

Conte¹,

Riccitiello²,

Luise³

et al. 2017

Biodegradable Polymers: Recent Developments and New Perspectives

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“…Disks (n=3/selected sample) were buffered for 24 hrs in 199 Media and fibroblasts were seeded onto the disks in 6-well plates at a density of 10 4 cells/well and incubated for 24 hrs. Cells were then fixed using the procedure from Wang et al [33] and imaged using a Quanta 200F Environmental Scanning Electron Microscope under a vacuum at a pressure of 0.60 torr. The electron beam was used at an accelerating voltage of 20 kV and a spot size of 4.0.…”

Section: Cell Adhesion Studiesmentioning

confidence: 99%

“…Standard treatment of nerve injury involves the administration of Methylprednisolone Sodium Succinate (MPSS) to reduce the inflammatory response, which addresses only one of the many impediments encountered by regenerating axons after injury [27,[30][31][32]. Recent studies have shown that bioactive glasses have the potential to encourage nerve regeneration after injury through mechanical guidance and delivery of growth factors [27,28,33]. Therefore, this work proposes the inclusion of Y 2 O 3 and CeO 2 into bioactive glass which may produce ROS scavenging capability similar to that observed in yttria and ceria nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Investigating the Effect of Glass Ion Release on the Cytocompatibility, Antibacterial Eflcacy and Antioxidant Activity of Y2O3 / CeO2 doped SiO2-SrO-Na2O glasses

et al. 2018

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Abstract:The effect on ion release and cytocompatibility of Yttrium (Y) and Cerium (Ce) are investigated when substituted for Sodium (Na) in a 0.52SiO 2 -0.24SrO-0.24-Na 2 O-MO glass series (where MO = Y 2 O 3 or CeO 2 ). Glass leaching was evaluated through pH measurements and Inductive Coupled Plasma-Optical Emission Spectrometry (ICP-OES) analysis where the extract pH increased during incubation (11.2 -12.5). Ion release of Silicon (Si), Na and Strontium (Sr) from the Con glass was at higher than that of glasses containing Y or Ce, and reached a limit after 1 day. Ion release from Y and Ce containing glasses reached a maximum of 1800 µg/mL, 1800 µg/mL, and 10 µg/mL for Si, Na, and Sr, respectively. Release of Y and Ce was below the ICP-OES detection limit <0.1 µg/mL. Cell viability of both L929 fibroblasts and MC3T3 osteoblasts decreased for Con, LY, and LCe extracts; HY extracts did not significantly decrease cell viability while YCe and HCe saw concentrationdependent viability decrease (20%, 33% extract concentrations). Bacterial studies saw Con and LCe eliminating >75% of bacteria at a 9% extract concentration. Antioxidant capacity (mechanism for neuroprotection) was evaluated using the ABTS assay. All glasses had inherent radical oxygen species (ROS) scavenging capability with Con reaching 9.5 mMTE.

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Varying the diameter of aligned electrospun fibers alters neurite outgrowth and Schwann cell migration

Cited by 276 publications

References 33 publications

Biomaterials for Enhancing Neuronal Repair

Biomaterials for Enhancing Neuronal Repair

Biodegradable polymers for dental tissue engineering and regeneration

Investigating the Effect of Glass Ion Release on the Cytocompatibility, Antibacterial Eflcacy and Antioxidant Activity of Y2O3 / CeO2 doped SiO2-SrO-Na2O glasses

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