Vascular and Excretory Effects of Angiotensin II in the Rat Isolated Perfused Kidney: Influence of an AT&lt;sub&gt;1&lt;/sub&gt; and a NonselectiveAT Receptor Antagonist

Hilchey, Sean D.; Bell-Quilley, Caroline P.

doi:10.1159/000028242

Cited by 5 publications

(3 citation statements)

References 16 publications

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“…The renoprotective action of enalapril is exerted mainly through decreased formation of AT [18] . Wang et al [19] found that enalapril significantly reduced the renal interstitial damage index.…”

Section: Discussionmentioning

confidence: 99%

Renoprotection against complete unilateral ureteric obstruction: Is there an ultimate choice?

Khalil

Shokeir

Wafa

et al. 2012

Arab Journal of Urology

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ObjectivesTo evaluate and compare the relative contribution of different therapeutic agents for renoprotection against complete unilateral ureteric obstruction (UUO), using a rabbit model sampled at different times.Materials and methodsEighty-four male New Zealand White rabbits were divided into seven groups of 12 rabbits; a sham group, a control (left UUO + no medication) or left UUO and treated with either enalapril, losartan, verapamil, l-arginine or antioxidant (vitamin E and selenium mixture). Rabbits in the control and treated groups were subjected to 3, 10 and 21 days of complete ureteric ligation and then killed humanely. The control and treated groups were evaluated at baseline and at the end of the experiment, by measuring split effective renal plasma flow (ERPF) using diuretic renography, and the split glomerular filtration rate (GFR) using selective creatinine clearance. Renal histopathology was evaluated using a tubulo-interstitial damage score.ResultsIn the sham group there was no significant effect on any of the evaluated variables. For split ERPF, losartan showed the highest renoprotective effect, saving 44% and 77% of ERPF at 3 and 21 days after UUO, respectively. Losartan was also the best renoprotective agent for GFR. For renal histopathology, enalapril showed the earliest and greatest improvement as assessed by the damage score, reaching 60% at 21 days after UUO. l-Arginine was the next best effect to blockade the renin-angiotensin system for renoprotection.ConclusionWe suggest that blockade of the renin-angiotensin system provides the best renoprotection against the effects of complete UUO.

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Section: Discussionmentioning

confidence: 99%

Renoprotection against complete unilateral ureteric obstruction: Is there an ultimate choice?

Khalil

Shokeir

Wafa

et al. 2012

Arab Journal of Urology

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“…In our model, 3 M bufalin induced a significant increase in sodium excretion with increased GFR but with no increase in RVR. At 10 M, the perfusion pressure was increased as well as sodium excretion and both GFR and RVR, as also observed after high concentrations of angiotensin II in the isolated kidney (17). Therefore, increased filtration, increased distal delivery, and decreased tubular reabsorption are probably involved in the bufalin natriuretic effect.…”

Section: Discussionmentioning

confidence: 60%

Natriuretic effect of bufalin in isolated rat kidneys involves activation of the Na⁺-K⁺-ATPase-Src kinase pathway

Arnaud-Batista

Costa

Oliveira

et al. 2012

American Journal of Physiology-Renal Physiology

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Bufadienolides are structurally related to the clinically relevant cardenolides (e.g., digoxin) and are now considered as endogenous steroid hormones. Binding of ouabain to Na(+)-K(+)-ATPase has been associated, in kidney cells, to the activation of the Src kinase pathway and Na(+)-K(+)-ATPase internalization. Nevertheless, whether the activation of this cascade also occurs with other cardiotonic steroids and leads to diuresis and natriuresis in the isolated intact kidney is still unknown. In the present work, we perfused rat kidneys for 120 min with bufalin (1, 3, or 10 μM) and measured its vascular and tubular effects. Thereafter, we probed the effect of 10 μM 3-(4-chlorophenyl)1-(1,1-dimethylethyl)-1H-pyrazolo[3,4-d]pyrimidin-4amine (PP2), a Src family kinase inhibitor, and 1,4-diamino-2,3-dicyano-1,4-bis[2-aminophenylthio] butadiene (UO126), a highly selective inhibitor of both MEK1 and MEK2, on bufalin-induced renal alterations. Bufalin at 3 and 10 μM profoundly increased several parameters of renal function in a time- and/or concentration-dependent fashion. At a concentration that produced similar inhibition of the rat kidney Na(+)-K(+)-ATPase, ouabain had a much smaller diuretic and natriuretic effect. Although bufalin fully inhibited the rat kidney Na(+)-K(+)-ATPase in vitro, its IC(50) (33 ± 1 μM) was threefold higher than the concentration used ex vivo and all its renal effects were blunted by PP2 and UO126. Furthermore, the phosphorylated (activated) ERK1/2 expression was increased after bufalin perfusion and this effect was totally prevented after PP2 pretreatment. The present study shows for the first time the direct diuretic, natriuretic, and kaliuretic effects of bufalin in isolated rat kidney and the relevance of Na(+)-K(+)-ATPase-mediated signal transduction.

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“…This suggests that angiotensin is also involved with functional tubular changes [17]. In UUO, ACE inhibitor prevented the increase in renal vascular resistance in response to angiotensin-II in the rat kidney [18].…”

Section: Discussionmentioning

confidence: 94%

Recoverability of renal function after relief of chronic partial unilateral ureteric obstruction: The effect of an angiotensin-converting enzyme inhibitor (enalapril)

Soliman

Shokeir

Mosbah

et al. 2011

Arab Journal of Urology

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ObjectivesWe evaluated the effect of an angiotensin-converting enzyme inhibitor (enalapril) on renal function during and after the relief of partial unilateral ureteric obstruction (UUO).Materials and methodsThirty-two male mongrel dogs were classified into three groups: sham (eight), control (12; left partial UUO + no medication) and study (12; left partial UUO + enalapril). Dogs in the study and control groups were subjected to 4 weeks of partial UUO. After that, the dogs were re-opened and subjected to Lich-Gregoir vesico-ureteric re-implantation, and were killed humanely by the end of the eighth week after relief of obstruction. The study and control groups were evaluated at baseline, after 4 weeks of obstruction and at 4 and 8 weeks after relief of obstruction, by measuring selective creatinine clearance (CCr), selective renographic clearance (RCr) and renal resistive index (RI). The sham group had sham surgery at 4 and 8 weeks and was evaluated as the other two groups.ResultsSham surgery showed no significant effect on any of the evaluated variables. Compared with the control, enalapril offset the reductions of CCr and RCr by an extra 11% and 12% of the basal values by the end of the fourth week of obstruction, respectively. Moreover, compared with the control, enalapril enhanced the recovery of CCr by an extra 10% and of RCr by an extra 23% of the basal values at 8 weeks after relief of the 4-week obstruction. In addition, the increase in RI was significantly less in the enalapril group.ConclusionEnalapril decreases the deterioration of renal function in partial UUO and enhances the recoverability of renal function after relief of obstruction.

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Vascular and Excretory Effects of Angiotensin II in the Rat Isolated Perfused Kidney: Influence of an AT<sub>1</sub> and a NonselectiveAT Receptor Antagonist

Cited by 5 publications

References 16 publications

Renoprotection against complete unilateral ureteric obstruction: Is there an ultimate choice?

Renoprotection against complete unilateral ureteric obstruction: Is there an ultimate choice?

Natriuretic effect of bufalin in isolated rat kidneys involves activation of the Na⁺-K⁺-ATPase-Src kinase pathway

Recoverability of renal function after relief of chronic partial unilateral ureteric obstruction: The effect of an angiotensin-converting enzyme inhibitor (enalapril)

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Vascular and Excretory Effects of Angiotensin II in the Rat Isolated Perfused Kidney: Influence of an AT<sub>1</sub> and a NonselectiveAT Receptor Antagonist

Cited by 5 publications

References 16 publications

Renoprotection against complete unilateral ureteric obstruction: Is there an ultimate choice?

Renoprotection against complete unilateral ureteric obstruction: Is there an ultimate choice?

Natriuretic effect of bufalin in isolated rat kidneys involves activation of the Na+-K+-ATPase-Src kinase pathway

Recoverability of renal function after relief of chronic partial unilateral ureteric obstruction: The effect of an angiotensin-converting enzyme inhibitor (enalapril)

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Natriuretic effect of bufalin in isolated rat kidneys involves activation of the Na⁺-K⁺-ATPase-Src kinase pathway