Vascular endothelial growth factor-A and -C protein up-regulation and early angiogenesis in a rat photothrombotic ring stroke model with spontaneous reperfusion

Gu, Weigang; Brännström, Thomas; Jiang, Wei; Bergh, Anders; Wester, Per

doi:10.1007/s004010100370

Cited by 47 publications

(28 citation statements)

References 45 publications

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“…Anti‐sense VEGFR‐2 had a similar effect, thus illustrating the importance of both receptors in adult angiogenesis. The finding that R1 is up‐regulated with ischemia during photothrombic ring stroke in rats with spontaneous reperfusion suggests that it may be involved in early events of angiogenesis (Gu et al, 2001). Thus, data regarding the precise contribution of R1 to the formation of vascular tubes may be dependent on (1) endothelial cell specificity, e.g., precursor, microvascular, or large vessel, (2) in vitro or in vivo conditions, and (3) organ specificity.…”

Section: Discussionmentioning

confidence: 99%

“…Such data suggest that signaling through R3 may be critical for vessel formation (Hamada et al, 2000). Experiments in rats with protothrombic ring stroke suggest that ischemia up‐regulates R3 and, accordingly, may play a role in angiogenesis (Gu et al, 2001). Although our in situ experiments did not lend themselves to identifying specific cell types that express mRNA for R3, the cells are most likely vascular or lymphatic endothelial cells or their precursors.…”

Section: Discussionmentioning

confidence: 99%

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Role of VEGF family members and receptors in coronary vessel formation

Tomanek

Holifield

Reiter

et al. 2002

Developmental Dynamics

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The specific roles of vascular endothelial growth factor (VEGF) family members and their receptors (VEGFRs) in coronary vessel formation were studied. By using the quail heart explant model, we found that neutralizing antibodies to VEGF-B or VEGF-C inhibited tube formation on the collagen gel more than anti-VEGF-A. Soluble VEGFR-1, a receptor for VEGF-A and -B, inhibited tube formation by 87%, a finding consistent with that of VEGF-B inhibition. In contrast, addition of soluble VEGFR-2, a receptor for VEGF family members A, C, D, and E, inhibited tube formation by only 43%. Acidic FGF-induced tube formation dependency on VEGF was demonstrated by the attenuating effect of a soluble VEGFR-1 and -2 chimera. The localization of VEGF R-2 and R-3 was demonstrated by in situ hybridization of serial sections, which documented marked accumulations of transcripts for both receptors at the base of the truncus arteriosus coinciding with the temporal and spatial formation of the coronary arteries by means of ingrowth of capillary plexuses. This finding suggests that both VEGFR-2 and R-3 may play a role in the formation of the coronary artery roots. In summary, these experiments document a role for multiple members of the VEGF family and their receptors in formation of the coronary vascular bed.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Role of VEGF family members and receptors in coronary vessel formation

Tomanek

Holifield

Reiter

et al. 2002

Developmental Dynamics

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“…This group and others showed that apoptosis within damaged EC might be necessary to regulate the process [66,67]. It has been reported that arteriolar collateral growth and new capillaries supported restored perfusion in the ischaemic border after ministroke [68], and in the cortical region after photothrombotic ring stroke in rats [69].…”

Section: Revascularisation and Tissue Reperfusion After Strokementioning

confidence: 91%

Gene activation and protein expression following ischaemic stroke: strategies towards neuroprotection

et al. 2005

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Current understanding of the patho-physiological events that follow acute ischaemic stroke suggests that treatment regimens could be improved by manipulation of gene transcription and protein activation, especially in the penumbra region adjacent to the infarct. An immediate reduction in excitotoxicity in response to hypoxia, as well as the subsequent inflammatory response, and beneficial control of reperfusion via collateral revascularization near the ischaemic border, together with greater control over apoptotic cell death, could improve neuronal survival and ultimately patient recovery. Highly significant differences in gene activation between animal models for stroke by middle cerebral artery occlusion, and stroke in patients, may explain why current treatment strategies based on animal models of stroke often fail. We have highlighted the complexities of cellular regulation and demonstrated a requirement for detailed studies examining cell specific protective mechanisms after stroke in humans.

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“…"Growing tracks" for nascent blood vessels also expand our knowledge of lymphatic functions. This study in zebrafish provides explanations why VEGF-C and VEGFR3 are up-regulated in the rat photothrombotic stroke model (Gu et al, 2001), and why arteriovenous-malformation human patients express lymphatic-associated genes in the brain (Shoemaker et al, 2014). Development of efficacious angiogenic therapies for ischemic stroke is dependent on a thorough understanding of brain vascular regeneration.…”

Section: Discussionmentioning

confidence: 97%

Cerebrovascular Injuries Induce Lymphatic Invasion into Brain Parenchyma to Guide Vascular Regeneration in Zebrafish

Chen

Yang

et al. 2018

Preprint

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Damage to regional brain vasculature and neuronal tissues occurs during acute cerebrovascular diseases, such as ischemic stroke. Promoting vascular regeneration is the most promising therapeutic approach. To understand cellular and molecular mechanisms underlying brain vascular regeneration, we developed two zebrafish cerebrovascular injury models using genetic ablation and photochemical thrombosis. Although brain parenchyma is physiologically devoid of lymphatic vasculature, we found that cerebrovascular injuries induce rapid ingrowth of meningeal lymphatics into the injured parenchyma. The ingrown lymphatics on one hand become lumenized All rights reserved. No reuse allowed without permission.(which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.

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Vascular endothelial growth factor-A and -C protein up-regulation and early angiogenesis in a rat photothrombotic ring stroke model with spontaneous reperfusion

Cited by 47 publications

References 45 publications

Role of VEGF family members and receptors in coronary vessel formation

Role of VEGF family members and receptors in coronary vessel formation

Gene activation and protein expression following ischaemic stroke: strategies towards neuroprotection

Cerebrovascular Injuries Induce Lymphatic Invasion into Brain Parenchyma to Guide Vascular Regeneration in Zebrafish

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