Vascular Endothelial Growth Factor Delivery via Gene Therapy for Diabetic Wounds: First Steps

Rico, Tace; Green, Jeremy; Kirsner, Robert S.

doi:10.1038/jid.2009.235

Cited by 14 publications

(4 citation statements)

References 18 publications

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“…We and others have demonstrated that VEGF plays a pathogenic role, e.g., in causing abnormal or pathologic angiogenesis and increasing vascular permeability that leads to enhanced release of inflammatory cytokines and infiltration of inflammatory cells in UC (Scaldaferri et al, 2009;Tolstanova et al, 2009). As shown previously, these angiogenic growth factors accelerated healing of upper gastrointestinal and skin ulcers by promoting normal or physiologic angiogenesis Kusstatscher et al, 1995;Szabo and Sandor, 1996;Deng et al, 2004;Rico et al, 2009).…”

Section: Introductionmentioning

confidence: 94%

Molecular Mechanisms of Basic Fibroblast Growth Factor Effect on Healing of Ulcerative Colitis in Rats

Paunovic¹,

Deng²,

Khomenko³

et al. 2011

J Pharmacol Exp Ther

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We demonstrated previously that basic fibroblast growth factor (bFGF) accelerated the healing of experimental duodenal ulcers, and we now hypothesize that bFGF might also accelerate the healing of experimental ulcerative colitis (UC). We also explored the potential molecular mechanisms involved in the accelerated healing of UC in rats treated with bFGF. The results demonstrated that colonic lesions were significantly reduced by bFGF treatment, whereas neutralization of bFGF aggravated iodoacetamide-induced UC. Protein expression of bFGF was increased during the healing stage of UC. Tumor necrosis factor-␣ levels and myeloperoxidase activity were significantly decreased in UC rats treated with bFGF, whereas they increased in rats treated with anti-bFGF antibody. Real-time polymerase chain reaction and immunohistochemistry showed decreased levels of p27 in the UC rats compared with the healthy controls, which was reversed by bFGF treatment in a dose-dependent manner. By immunohistochemistry and double labeling of Ki-67 and CD34, prominent positive staining of Ki-67 and CD34 was seen after bFGF treatment, indicating the enhanced proliferation of fibroblasts and epithelial and endothelial cells, i.e., angiogenesis. We conclude that bFGF plays a beneficial role in the healing of UC in rats. The molecular mechanisms of bFGF in UC healing not only involve the expected increased cell proliferation, especially angiogenesis, but also encompass the reduction of inflammatory cytokines and infiltration of inflammatory cells. Thus, bFGF enema may be a new therapeutic option for UC.

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Section: Introductionmentioning

confidence: 94%

Molecular Mechanisms of Basic Fibroblast Growth Factor Effect on Healing of Ulcerative Colitis in Rats

Paunovic¹,

Deng²,

Khomenko³

et al. 2011

J Pharmacol Exp Ther

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“…9,10,11 Some growth factors such as Epidermal growth factors, granulocyte colony stimulating factor, nerve growth factor, vascular endothelial growth factor activated platelet rich plasma were evaluated in diabetic foot ulcers. 12 Platelet derived growth factor PDGF is a dimeric protein, composed of 2 disulfide -linked polypeptide chain. It exists in 3 different isomers , the heterodimer PDGF -ab consisting of an a and b chain , and two homodimers, consisting 2a or 2b chains (pdgf -aa and pdgf -bb) it has been shown in preclinical and clinical studies to promote the formation of granualation tissue, wound site and to stimulate wound healing.…”

Section: Introductionmentioning

confidence: 99%

Prospective study of platelet derived growth factor in wound healing of diabetic foot ulcers in Indian population

Rangaswamy¹,

Rubby²,

Baalann³

2017

Int Surg J

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“…Some biological therapies were also evaluated in diabetic foot ulcer treatment. Some growth factors such as Epidermal growth factor 13,14 , Granulocyte colony stimulating factor 15 , Nerve growth factor 16 , Vascular endothelial growth factor 17 , activated platelet rich plasma 6 were evaluated in diabetic foot ulcers.…”

Section: Introductionmentioning

confidence: 99%

Untitled

2016

IJPSR

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Background-In September 2012, the world health organization reported a global prevalence of diabetes exceeding 300 million people, predicting a further 60-70% increase by the year 2030, which means India alone will have 100 million people by year 2030. Wound healing is problematic in diabetic patients. Encouraging results have shown that PDGF application is better than good wound care alone. But the evidence to demonstrate the safety and efficacy of PDGF in Diabetic ulcer is scanty. Aim-To Study the Efficacy of Platelet Derived Growth Factor in Diabetic Lower Extremity Ulcer. Methods-This study was a prospective, double blind, and placebo controlled study. A total of 29 patients with 35 ulcers were included and divided in case and control group. The study medication was administered in conjunction with standardized good wound care for 24 weeks or until target ulcer healed. Results-It was found that 74.2% of ulcers were neuropathic while 48.3% patients had foot deformity in this study. At the end of 24 weeks 100% ulcers in group receiving PDGF completely healed while group receiving Placebo 76.4% ulcers healed. Mean of time to achieve wound healing was 9 + 7.1 weeks in ulcer receiving PDGF gel and they healed 50% faster as compared to ulcers receiving placebo. Conclusion-There was no relation of type, duration, ABI, neurological deficit, type of ulcer, foot deformity, wagner grade, edema, infection, duration of ulcer with time to achieve wound closure. This meant that PDGF was solely responsible for the ulcers to heal 50% faster.

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Vascular Endothelial Growth Factor Delivery via Gene Therapy for Diabetic Wounds: First Steps

Cited by 14 publications

References 18 publications

Molecular Mechanisms of Basic Fibroblast Growth Factor Effect on Healing of Ulcerative Colitis in Rats

Molecular Mechanisms of Basic Fibroblast Growth Factor Effect on Healing of Ulcerative Colitis in Rats

Prospective study of platelet derived growth factor in wound healing of diabetic foot ulcers in Indian population

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