Vascular endothelial growth factor immobilized in collagen scaffold promotes penetration and proliferation of endothelial cells

Shen, Yi; Shoichet, Molly S.; Radišić, Milica

doi:10.1016/j.actbio.2007.12.011

Cited by 263 publications

(221 citation statements)

References 45 publications

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“…al. observed increased endothelial cell number and infiltration on collagen scaffolds uniformly immobilized with VEGF [52].…”

Section: Vascular Endothelial Growth Factormentioning

confidence: 86%

“…This is a highly porous collagen sponge with pore size ranging between 100-200 µm [52]. The sterile sheet of the scaffold was cut into the desired shape, circle or rectangle, with either a circular autoclaved metal cork borer or autoclaved metal scissors, respectively.…”

Section: Scaffold Preparationmentioning

confidence: 99%

“…They are from an embryoid body-derived mouse EC line and are propagated in culture medium (D4T medium) consisting of Iscove's Modified Dulbecco's Media (IMDM, Gibco, 12440-053) with 5% fetal bovine serum (FBS, Gibco, 16000-044), 100 units/mL penicillin, and 100 µg/mL streptomycin (Gibco/Invitrogen, 15140122) [65]. This cell line has previously been used by our lab for cardiac tissue engineering studies [52,66]. The cells used in this study are between the passages of 10 and 20.…”

Section: Cell Studies 271 Cell Culturementioning

confidence: 99%

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Endothelial cells guided by immobilized gradients of vascular endothelial growth factor on porous collagen scaffolds

et al. 2011

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A key challenge in tissue engineering is overcoming cell death in the scaffold interior due to the limited diffusion of oxygen and nutrients therein. We hypothesized here that immobilizing a gradient of vascular endothelial growth factor would guide endothelial cells into the interior of the scaffold thereby enhancing angiogenesis. The protein was immobilized onto a collagen scaffold through carbodiimide chemistry by one of the three methods experimented: placing 5 µl of the solution at the center of the scaffold to create a ~2 ng/ml/mm gradient in a radial direction. D4T endothelial cells were observed to be guided by this VEGF-165 gradient deep into the center of the scaffold compared to both uniformly immobilized VEGF-165 and VEGFfree controls. We concluded that the VEGF-165 gradient scaffolds promoted the migration, and not proliferation, of cells deep into the scaffold. These gradient scaffolds provide the foundation for future in vivo tissue engineering studies.iii

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“…al. observed increased endothelial cell number and infiltration on collagen scaffolds uniformly immobilized with VEGF [52].…”

Section: Vascular Endothelial Growth Factormentioning

confidence: 86%

Section: Scaffold Preparationmentioning

confidence: 99%

Section: Cell Studies 271 Cell Culturementioning

confidence: 99%

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Endothelial cells guided by immobilized gradients of vascular endothelial growth factor on porous collagen scaffolds

et al. 2011

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“…After promising studies with P(LLA-co-CL) [10] and an established spray coating process (data not shown) we further evaluate a dual drug delivery system for DES to improve reendothelialization. As model drug for PTX we used FDAc due to the similar diffusion and distribution coefficients [11].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, we developed a dual local drug delivery coating based on poly(L-lactide-co-ε-caprolactone) (PLLAco-CL) with vascular endothelial growth factor (VEGF 165 ), a potent mitogen for endothelial cells [10], for surface immobilization. As model drug for bulk incorporation we used fluorescein diacetate (FDAc) with diffusion and distribution coefficients similar to paclitaxel [11].…”

Section: Introductionmentioning

confidence: 99%

Mutual influence of incorporated drugs in a dual drug delivery coating for cardiovascular applications

Wulf¹,

Teske²,

Matschegewski³

et al. 2017

Current Directions in Biomedical Engineering

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For a long-term efficient drug-eluting stent for vascular applications, the development of drug-loaded coating, combining the effective inhibition of smooth muscle cell proliferation while promoting the re-endothelialization, is a promising concept. However the mostly required simultaneous incorporation of drugs can influence decisively the stability, efficacy and release of the respective drug. Therefore, the mutual influence of a dual local drug delivery coatings based on poly(L-lactide-co-ε-caprolactone) (PLLAco-CL) containing vascular endothelial growth factor (VEGF 165 ) coupled to the surface and an embedded drug, such as fluorescein diacetate (FDAc) instead of Paclitaxel (PTX) on the in vitro drug release was investigated. Surprisingly, for the investigated coating the immobilized VEGF loading was enhanced and the release profile was accelerated by FDAc incorporation. Even a manifold increase for the in vitro released amounts of VEGF was detected. In contrast, the immobilization of VEGF seems to have a negligible influence on the in vitro FDAc release profiles.

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Tissue Engineering Biomaterials

Peter

Eyster

Doleyres

2018

Encyclopedia of Polymer Science and Technology

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Tissue engineering has shown great promise in developing novel therapies for treating injured tissue and organ failure. Scaffolds play a pivotal role in tissue engineering. Biodegradable polymers are the primary choice of material for tissue engineering scaffolds, due to their numerous advantageous properties. In this article, the principles of tissue engineering are discussed in relation to polymer science and engineering. The most frequently used polymers in tissue engineering are briefly reviewed. These include synthetic and natural polymers commonly used in constructing both porous and hydrogel scaffolds. Their structure and critical properties in relation to scaffold function are discussed in depth, along with a detailed review of the important roles functionalized materials and controlled release play in tissue engineering. Important polymer processing techniques in the context of scaffold fabrication are also reviewed. These include the textile technologies, electrospinning, particulate‐leaching techniques, phase‐separation techniques, rapid prototyping, and other novel three‐dimensional (3D) fabrication techniques. In this update, the impact of pluripotent stem cells, conductive polymers, controlled drug release, 3D printing, and nanofibrous topology on tissue engineering are also discussed.

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Vascular endothelial growth factor immobilized in collagen scaffold promotes penetration and proliferation of endothelial cells

Cited by 263 publications

References 45 publications

Endothelial cells guided by immobilized gradients of vascular endothelial growth factor on porous collagen scaffolds

Endothelial cells guided by immobilized gradients of vascular endothelial growth factor on porous collagen scaffolds

Mutual influence of incorporated drugs in a dual drug delivery coating for cardiovascular applications

Tissue Engineering Biomaterials

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