Vascular Endothelial Growth Factor In Ocular Fluid of Patients with Diabetic Retinopathy and Other Retinal Disorders

Aiello, Lloyd Paul; Avery, Robert L.; Arrigg, Paul G.; Keyt, Bruce A.; Jampel, Henry D.; Shah, Sabera T.; Pasquale, Louis R.; Thieme, Hagen; Iwamoto, M.; Park, J E

doi:10.1097/00006982-199515020-00026

Cited by 388 publications

(523 citation statements)

References 0 publications

Supporting

Mentioning

479

Contrasting

Unclassified

Order By: Relevance

“…The major contributing factor underlying the proliferation of choroidal blood vessels is the upregulation of VEGF, where increased levels have been found in the vitreous of patients suffering from AMD. 2 Recent research has, therefore, centered on the control of VEGF expression or on limiting its biological activity. [14][15][16] ODNs have recently come to the forefront of technology for the control of gene expression but some issues still need to be addressed.…”

Section: Discussionmentioning

confidence: 99%

“…Overexpression of vascular endothelial growth factor (VEGF) has been implicated in a number of diseases associated with angiogenesis including age-related macular degeneration (AMD), 1 diabetic retinopathy (DR) 2,3 and tumor development. 4 Several gene therapies are being developed and trialed for the control of such diseases and much of the technology centers on the inhibition of VEGF and consequently the inhibition of angiogenesis, a process that is essential to disease progression.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Dendrimer delivery of an anti-VEGF oligonucleotide into the eye: a long-term study into inhibition of laser-induced CNV, distribution, uptake and toxicity

et al. 2005

View full text Add to dashboard Cite

We have performed a long-term study into the use of a lipophilic amino-acid dendrimer to deliver an anti-vascular endothelial growth factor (VEGF) oligonucleotide (ODN-1) into the eyes of rats and inhibit laser-induced choroidal neovascularization (CNV). In addition, the uptake, distribution and retinal tolerance of the dendrimer plus oligonucleotide conjugates were examined. Analysis of fluorescein angiograms of laser photocoagulated eyes revealed that dendrimer plus ODN-1 significantly inhibited (Po0.05) the development of CNV for 4-6 months by up to 95% in the initial stages. Eyes similarly injected with ODN-1 alone showed no significant difference (P40.05) in mean severity score at 2 months (2.8670.09), 4 months (2.1570.17) or 6 months (2.770.12) compared to the vehicle-injected controls. Furthermore, we showed that intravitreally injected ODN-1 tagged with 6-fam was absorbed by a wide area of the retina and penetrated all of the retinal cell layers to the retinal pigment epithelium. Ophthalmological examinations indicated that the dendrimers plus ODN-1 conjugates were well tolerated in vivo, which was later confirmed using immunohistochemistry, which showed no observable increase in antigens associated with inflammation. We conclude that the use of such dendrimers may provide a viable mechanism for the delivery of therapeutic oligonucleotides for the treatment of angiogenic eye diseases. Gene Therapy (2005) 12, 1544-1550.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dendrimer delivery of an anti-VEGF oligonucleotide into the eye: a long-term study into inhibition of laser-induced CNV, distribution, uptake and toxicity

et al. 2005

View full text Add to dashboard Cite

show abstract

“…2,3 It has been suggested that IL-6 may indirectly induce angiogenesis by stimulating VEGF expression, as occurs with the induction of VEGF mRNA by hypoxia. 9 It has been suggested that induction of VEGF mRNA probably occurs through transcriptional regulation, so previous reports combined with our results would indicate that IL-6 may affect neovascularization and the progression of PDR after vitreous surgery by upregulating expression of VEGF.…”

Section: Discussionmentioning

confidence: 99%

“…All three fundus parameters (NVE, NVD, and FPE) reflect retinal neovascularization, while VEGF and IL-6 are involved in the onset and progression of new vessel formation. 1,2,9 It was suggested that the additional laser was needed to reduce the activity of new vessel formation during surgery in the advanced cases. Therefore, active neovascularization is associated with the progression of PDR.…”

Section: Eyementioning

confidence: 99%

“…1 Vascular endothelial growth factor (VEGF) is an angiogenic and permeabilityenhancing glycoprotein that predominantly acts on endothelial cells, and VEGF has been shown to increase in patients with active intraocular neovascularization. [1][2][3] Recently, it has been suggested that chronic subclinical inflammation may underlie much of the vascular pathology of diabetic retinopathy. 4 Interleukin-6 (IL-6) is a multifunctional cytokine 5 that may be a major mediator of anterior uveitis 6 and proliferative vitreoretinopathy (PVR).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Risk evaluation of outcome of vitreous surgery based on vitreous levels of cytokines

Funatsu

Yamashita

Mimura³

et al. 2006

Eye

View full text Add to dashboard Cite

show abstract

Monocyte Chemoattractant Protein 1, Intercellular Adhesion Molecule 1, and Vascular Cell Adhesion Molecule 1 in Exudative Age-Related Macular Degeneration

Jonas

Tao²,

Neumaier³

et al. 2010

Arch Ophthalmol

131

View full text Add to dashboard Cite

To examine intraocular concentrations of monocyte chemoattractant protein 1 (MCP-1), soluble intercellular adhesion molecule 1 (sICAM-1), soluble vascular cell adhesion molecule 1 (sVCAM-1), and vascular endothelial growth factor (VEGF) in eyes with exudative age-related macular degeneration (AMD). Methods:The investigation included a study group of 28 patients (28 eyes) with exudative AMD and a control group of 25 patients (25 eyes) with cataract. The concentrations of MCP-1, sICAM-1, sVCAM-1, and VEGF in aqueous humor samples obtained during surgery were measured using a solid-phase chemiluminescence immunoassay. Results: The study group as compared with the control group had higher aqueous concentrations of sICAM-1 (mean [SD], 844 [2073] vs 246 [206] pg/mL, respectively; PϽ.001), sVCAM-1 (mean [SD], 7978 [7120] vs 2999 [1426] pg/mL, respectively; PϽ.001), and MCP-1 (mean [SD], 587 [338] vs 435 [221] pg/mL, respectively; P=.07). The concentration of VEGF did not vary significantly between the groups (P=.76). The MCP-1 concentration was significantly associated with macular thickness (r=0.40; P=.004). It decreased significantly with the type of subfoveal neovascular membrane (classic membrane type, occult membrane, retinal pigment epithelium detachment) (P=.009). The concentrations of sICAM-1, sVCAM-1, and VEGF were not significantly associated with membrane type and macular thickness (PՆ.18). Conclusions: Concentrations of MCP-1, sICAM-1, and sVCAM-1 are significantly associated with exudative AMD, even in the presence of normal VEGF concentrations. Intraocular MCP-1 concentrations are correlated with the subfoveal neovascular membrane type and the amount of macular edema. One may infer that MCP-1, sICAM-1, and sVCAM-1 could potentially be additional target molecules in therapy for exudative AMD.

show abstract

Vascular Endothelial Growth Factor In Ocular Fluid of Patients with Diabetic Retinopathy and Other Retinal Disorders

Cited by 388 publications

References 0 publications

Dendrimer delivery of an anti-VEGF oligonucleotide into the eye: a long-term study into inhibition of laser-induced CNV, distribution, uptake and toxicity

Dendrimer delivery of an anti-VEGF oligonucleotide into the eye: a long-term study into inhibition of laser-induced CNV, distribution, uptake and toxicity

Risk evaluation of outcome of vitreous surgery based on vitreous levels of cytokines

Monocyte Chemoattractant Protein 1, Intercellular Adhesion Molecule 1, and Vascular Cell Adhesion Molecule 1 in Exudative Age-Related Macular Degeneration

Contact Info

Product

Resources

About