Vascular endothelial growth factor in bronchoalveolar lavage from normal subjects and patients with diffuse parenchymal lung disease

Meyer, Keith C.; Cardoni, Andrew L.; Xiang, Zhuzai

doi:10.1067/mlc.2000.105618

Cited by 78 publications

(68 citation statements)

References 39 publications

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“…Therefore, there is a limitation in the measurement of VEGF levels in this study since these data were not normalised. MEYER et al [15] have demonstrated that the VEGF levels in bronchoalveolar fluid decline with age in normal subjects. However, in this study, emphysema patients and normal controls were well matched with respect to age, although it is possible that ageing is an important factor that influences the VEGF levels in induced sputum.…”

Section: Discussionmentioning

confidence: 99%

Imbalance between vascular endothelial growth factor and endostatin in emphysema

Kanazawa

Hirata²,

Yoshikawa³

2003

Eur Respir J

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Vascular endothelial growth factor (VEGF) stimulates endothelial cell proliferation, and endostatin directly antagonises the biological effects of VEGF. The maintenance of pulmonary endothelial cells is also thought to depend upon the local balance of VEGF and endostatin in the lung. Therefore, this study was designed to determine whether there is an imbalance between VEGF and endostatin levels in patients with pulmonary emphysema.VEGF and endostatin levels were simultaneously measured from 25 emphysema patients and 12 normal control subjects, and their correlation and balance in induced sputum was evaluated.VEGF levels in induced sputum were significantly lower in emphysema patients (854±307 pg·mL−1) than in normal controls (1,791±1,192 pg·mL−1). In contrast, there was no significant difference in endostatin levels among the two groups. Therefore, the ratio of VEGF to endostatin levels was markedly lower in emphysema patients (4.5±1.8) than in normal controls (8.1±2.6). Moreover, VEGF levels were correlated with endostatin levels in normal controls but not in emphysema patients. In addition, the ratio of VEGF to endostatin levels was correlated with forced expiratory volume in one second (FEV1), FEV1/forced vital capacity and carbon monoxide diffusing capacity of the lung in emphysema patients.The findings in this study suggest that there is an imbalance between vascular endothelial growth factor and endostatin levels in induced sputum from emphysema patients.

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Section: Discussionmentioning

confidence: 99%

Imbalance between vascular endothelial growth factor and endostatin in emphysema

Kanazawa

Hirata²,

Yoshikawa³

2003

Eur Respir J

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“…BALUK et al [11] have shown that selective expression of VEGF-A in mouse airway epithelium triggered tracheal angiogenesis. Previous studies in CF subjects have reported increased VEGF-A concentrations in serum [12][13][14], but little is known about VEGF-A expression in CF airways. KRENN et al [15] found increased VEGF-A mRNA and protein in lung homogenates of CF subjects undergoing lung transplantation.…”

Section: Introductionmentioning

confidence: 99%

CFTR dysfunction induces vascular endothelial growth factor synthesis in airway epithelium

Martin

Coolen

et al. 2013

Eur Respir J

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Peribronchial angiogenesis may occur in cystic fibrosis and vascular endothelial growth factor (VEGF)-A regulates angiogenesis in airways.Peribronchial vascularity and VEGF-A expression were examined using immunocytochemistry and morphometric analysis in lung sections obtained in 10 cystic fibrosis patients at transplantationversus10 control nonsmokers, and in two strains of Cftr-deficient mice versus wild-type littermates. Airway epithelial NCI-H292 cells and primary cultures of noncystic fibrosis human airway epithelial cells were treated with cystic fibrosis transmembrane conductance regulator (CFTR) inhibitors (CFTR-inh172or PPQ-102) or transfected with a CFTR small interfering (si)RNA with or without a selective epidermal growth factor receptor tyrosine kinase inhibitor. Concentrations of VEGF-A and phosphorylated epidermal growth factor receptor were measured by ELISA.Peribronchial vascularity was increased in cystic fibrosis patients, but not inCftr-deficient mice. VEGF-A immunostaining was localised to airway epithelium and was increased in cystic fibrosis patients and inCftr-deficient mice. In cultured airway epithelial cells, treatment with CFTR inhibitors or transfection with CFTR siRNA induced a twofold increase in VEGF-A production. CFTR inhibitors triggered epidermal growth factor receptor phosphorylation that was required for VEGF-A synthesis.Cystic fibrosis airways at transplantation showed increased peribronchial vascularity and epithelial VEGF-A expression. CFTR dysfunction triggered epithelial synthesis of VEGF-A, which may contribute to vascular remodelling.

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“…However, baseline plasma levels of VEGF 39 are significantly related to the extent of parenchymal involvement in high-resolution computed tomography, and patients with IPF who develop progressive disease have significantly higher baseline levels of VEGF. 39 In contrast, bronchoalveolar lavage fluid concentrations of VEGF are significantly depressed in patients with IPF [40][41][42] and correlate with diffusing capacity of the lung for carbon monoxide (D LCO ). The latter correlation possibly reflects the diminished epithelial surface area versus the diminished gene expression or intraluminal secretion of VEGF.…”

Section: Clinical Datamentioning

confidence: 98%

“…The latter correlation possibly reflects the diminished epithelial surface area versus the diminished gene expression or intraluminal secretion of VEGF. 42 …”

Section: Clinical Datamentioning

confidence: 99%

Nintedanib: A Novel Therapeutic Approach for Idiopathic Pulmonary Fibrosis

Dimitroulis

2014

Respir Care

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Vascular endothelial growth factor in bronchoalveolar lavage from normal subjects and patients with diffuse parenchymal lung disease

Cited by 78 publications

References 39 publications

Imbalance between vascular endothelial growth factor and endostatin in emphysema

Imbalance between vascular endothelial growth factor and endostatin in emphysema

CFTR dysfunction induces vascular endothelial growth factor synthesis in airway epithelium

Nintedanib: A Novel Therapeutic Approach for Idiopathic Pulmonary Fibrosis

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