Vascular endothelial growth factor, nitric oxide, and leptin follicular fluid levels correlate negatively with embryo quality in IVF patients

Barroso, Gerardo; Barrionuevo, Marcelo J.; Rao, P. S.; Graham, Lloyd B.; Danforth, Douglas R.; Huey, Suzanne; Abuhamad, Alfred; Oehninger, Sergio

doi:10.1016/s0015-0282(99)00442-2

Cited by 131 publications

(70 citation statements)

References 8 publications

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“…Previous studies have suggested that an imbalance in endometrial angiogenesis and a too rapidly formed vascular network may result in failed implantation and first-trimester miscarriage [Banerjee et al 2013;Lash et al 2012;Plaisier et al 2009;Su et al 2011]. Furthermore, analyses of fertility-related soluble mediators in human uterine fluid have identified the angiogenic factor VEGF as a key regulator of embryo implantation [Hannan et al 2011], with high levels of VEGF in follicular fluid being associated with low fertilization rates [Malamitsi-Puchner et al 2001], and poor quality embryos [Barroso et al 1999]. HRG has been reported to interact with molecules such as TSP, VEGF, and various members of the fibroblast growth factor family [Jones et al 2005;Wake et al 2009].…”

Section: Discussionmentioning

confidence: 99%

Histidine-rich glycoprotein derived peptides affect endometrial angiogenesisin vitrobut has no effect on embryo development

Lindgren

Hreinsson

Helmestam

et al. 2016

Systems Biology in Reproductive Medicine

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(2016) Histidine-rich glycoprotein derived peptides affect endometrial angiogenesis invitro but has no effect on embryo development, Systems Biology in Reproductive Medicine, 62:3, 192-200, DOI: 10.3109/19396368.2016.1156785 To link to this article: https://doi.org/10. 3109/19396368.2016 Histidine-rich glycoprotein (HRG) is an abundant plasma protein involved in multiple biological processes including immunology, vascularisation, and coagulation. These processes are of importance in regulating embryo development and implantation. A specific polymorphism in the HRG gene, HRG C633T, has an impact on various aspects of fertility, such as oocyte quality, endometrial receptivity, and possibly the capacity of the embryo itself to implant. To further examine the potential role of the HRG C633T polymorphism in regulating endometrial angiogenesis and on embryo development, two HRG peptides were constructed. These HRG peptides correspond to the amino acids 169-203 of the protein which, in turn, reflects the C633T polymorphism in the gene. The HRG proline or serine peptides were added to cultures of primary human endometrial endothelial (HEE) cells and to human embryos in vitro. The HRG peptides inhibited vascular endothelial growth factor (VEGF) induced proliferation and migration and promoted tube formation of HEE cells. The embryos were monitored using a time-lapse system (EmbryoScope®). Except for a prolonged time from first cleavage after thawing to development of the morula, no difference in embryo morphokinetics or embryo quality was noted in human embryos cultured in the presence of the HRG proline peptide. Taken together, these results suggest that treatment with a specific HRG peptide might prime the endometrium for implantation and be beneficial for adequate placentation. However, addition of a specific HRG proline peptide to human embryos has no beneficial effects in terms of embryo development.

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Section: Discussionmentioning

confidence: 99%

Histidine-rich glycoprotein derived peptides affect endometrial angiogenesisin vitrobut has no effect on embryo development

Lindgren

Hreinsson

Helmestam

et al. 2016

Systems Biology in Reproductive Medicine

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“…On the other hand, we found lower FF VEGF levels in the group of pregnancy than those from the group of no pregnancy in PCOS women, and our finding is in agreement with Nikolettos et al (26) and Mary et al (22) who found that elevated concentrations of VEGF in follicular fluid correlate negatively with conception rates in assisted reproductive technologies. This negative impact of VEGF on pregnancy outcome may be explained by the finding of Barroso et al (27) who found that follicular VEGF levels are negatively-correlated with embryo morphology. VEGF concentration in follicular fluid is said to be dependent on the quality and number of granulosa cells responding (28) .…”

Section: Discussion:-mentioning

confidence: 99%

“…VEGF concentration in follicular fluid is said to be dependent on the quality and number of granulosa cells responding (28) . Greater granulosa cell numbers have been associated with more competent follicles and lower follicular fluid VEGF levels with more oocytes (29) and better embryo quality (4,27) . Van Blerkom et al (28) demonstrated that VEGF is associated with follicular vascularization and oxygenation and those oocytes from severely hypoxic follicles ascertained by lower VEGF concentrations are associated with higher frequencies of abnormalities in chromosomal organization which can lead to disorders in developing embryos.…”

Section: Discussion:-mentioning

confidence: 99%

The Effect of Different Ovarian Stimulation Protocols on Vascular Endothelial Growth Factor (Vegf) Concentration in the Serum and Follicular Fluid of Women With Pcos Undergoing Icsi/Ivf.

Aboudy¹,

RedaAlKawaz²,

Ani³

2017

IJAR

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Objective:To measure the concentration of vascular endothelial growth factor (VEGF) in serum and follicular fluid of patients with PCOS at day of ova pick up after using of different IVF/ICSI protocols and to assess the pregnancy outcome. Study design: A total of 90 infertile women (55 women with Polycystic ovary syndrome and the other 35 women as a control (non PCOS) undergoing controlled ovarian hyperstimulation for intracytoplasmic sperm injection cycle were prospectively recruited for this study in High Institute of Infertility Diagnosis and Assisted Reproductive Technology / AL-Nahrian University and Kamal ALSamarai Hospital, center of fertility and IVF (Baghdad/Iraq) during the period from December 2015 to the end of April 2017.The fifty five PCOS women were divided into three groups: twenty five (25) infertile women have had PCOS undergoing long Agonist protocol for ICSI cycle, fifteen (15) infertile women have had PCOS undergoing Antagonist protocol for ICSI and fifteen (15) infertile women have had PCOS undergoing short Agonist protocol for ICSI. The controlled group included 35 women were free of signs and symptoms of PCOS with regular cycles and no endocrine abnormalities undergoing ICSI cycle (15 infertile women underwent long agonist protocol, 10 women underwent short agonist and 10 women underwent antagonist protocol). In all patients, serum and follicular fluid VEGF levels were measured on the day of oocyte retrieval by using Enzyme linked immuno sorbent assay.

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“…Most authors agree that low concentrations of VEGF in follicular fluid are found in good prognosis ART patients (as determined by peak serum estradiol concentrations, number of gonadotropin ampoules administered per cycle, number of oocytes retrieved, embryo quality and pregnancy rate) [22,23]. In contrast, high levels of VEGF production by GC cultures are found in patients with elevated concentrations of serum estradiol, higher number of follicles and oocytes retrieved, fertilization and pregnancy rates [24,25].…”

Section: Discussionmentioning

confidence: 99%

Significance of ovarian macrophages in the follicular aspirates from ART patients

Barañao

Quintana²,

Martín

et al. 2007

J Assist Reprod Genet

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Purpose: To evaluate the percentages of macrophages present in granulosa cells (GC) cultures from patients with different responses to the hyperstimulation, in relation to the percentages of apoptotic cells (ApC), as well as to the release of cytokines.Methods: We studied 42 patients: 12 Hyporesponders, (with ≤ 4 follicles), 15 Normoresponders, (5-14 follicles), and 15 Hyperresponders, ( ≥15 follicles). In GC cultures percentages of macrophages and ApC were counted and, in the conditioned media, cytokines were measuredResults: Percentages of macrophages were significantly higher in GC cultures from Hyporesponders compared with Hyperresponders patients. Also, the percentages of ApC cells were the highest in Hyporesponders. On the contrary, cytokines concentrations were the lowest in this group.Conclusions: The low ovarian response is probably due to the decreased angiogenesis, which in turn produces in-

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Vascular endothelial growth factor, nitric oxide, and leptin follicular fluid levels correlate negatively with embryo quality in IVF patients

Cited by 131 publications

References 8 publications

Histidine-rich glycoprotein derived peptides affect endometrial angiogenesisin vitrobut has no effect on embryo development

Histidine-rich glycoprotein derived peptides affect endometrial angiogenesisin vitrobut has no effect on embryo development

The Effect of Different Ovarian Stimulation Protocols on Vascular Endothelial Growth Factor (Vegf) Concentration in the Serum and Follicular Fluid of Women With Pcos Undergoing Icsi/Ivf.

Significance of ovarian macrophages in the follicular aspirates from ART patients

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