Vascular Endothelial Growth Factor Receptor 2-Specific Microbubbles for Molecular Ultrasound Detection of Prostate Cancer in a Rat Model

Fischer, Thomas; Thomas, Anke; Tardy, Isabell; Schneider, Michel; Hünigen, Hana; Custodis, Pia; Beyersdorff, Dirk; Plendl, Johanna; Schnorr, Jörg; Diekmann, Felix; Gemeinhardt, Ole

doi:10.1097/rli.0b013e3181efd6b2

Cited by 37 publications

(25 citation statements)

References 44 publications

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“…Studies in mice and rats have shown promising results for different types of cancer. For prostate cancer, microbubbles targeting vascular endothelial growth factor receptor 2 (VEGFR2) have been successfully tested [52, 53]. Contrast may be further enhanced by using dual-targeted microbubbles, which have a higher binding efficiency, as shown by Willmann et al who targeted αvβ3 integrin as well as VEGFR2 [54].…”

Section: Molecular Imaging Applications In Cancer Diagnosis and Stmentioning

confidence: 99%

Molecular imaging for cancer diagnosis and surgery

Hussain

Nguyen

2014

Advanced Drug Delivery Reviews

280

236

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Novel molecular imaging techniques have the potential to significantly enhance the diagnostic and therapeutic approaches for cancer treatment. For solid tumors in particular, novel molecular enhancers for imaging modalities such as US, CT, MRI and PET may facilitate earlier and more accurate diagnosis and staging which are prerequisites for successful surgical therapy. Enzymatically activatable “smart” molecular MRI probes seem particularly promising because of their potential to image tumors before and after surgical removal without re-administration of the probe to evaluate completeness of surgical resection. Furthermore, the use of “smart” MR probes as part of screening programs may enable detection of small tumors throughout the body in at-risk patient populations. Dual labeling of molecular MR probes with fluorescent dyes can add real time intraoperative guidance facilitating complete tumor resection and preservation of important structures. A truly theranostic approach with the further addition of therapeutic agents to the molecular probe for adjuvant therapy is conceivable for the future.

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Section: Molecular Imaging Applications In Cancer Diagnosis and Stmentioning

confidence: 99%

Molecular imaging for cancer diagnosis and surgery

Hussain

Nguyen

2014

Advanced Drug Delivery Reviews

280

236

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“…In addition, with the assistance of ARF, recent studies have also demonstrated ultrasound targeted molecular imaging in large vessel environments using some new techniques(Mauldin et al, 2012; Patil et al, 2011; Wang et al, 2013b). In summary, state-of-the-art ultrasound molecular imaging methods have demonstrated efficacy in pre-clinical studies for detection and monitoring of various types of cancers (Bzyl et al, 2011; Fischer et al, 2010; Pysz et al, 2010)as well as cardiovascular inflammation (Kaufmann et al, 2007; Masseau et al, 2012). However, these approaches possess some limitations such as requiring a “control injection” (i.e.…”

Section: Introductionmentioning

confidence: 99%

Ultrasound-Based Measurement of Molecular Marker Concentration in Large Blood Vessels: A Feasibility Study

Wang

Mauldin

Klibanov

et al. 2015

Ultrasound in Medicine & Biology

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Ultrasound molecular imaging has demonstrated efficacy in pre-clinical studies for cancer and cardiovascular inflammation. However, these techniques often require lengthy protocols due to waiting periods or additional control microbubble injections. Moreover, they are not capable of quantifying molecular marker concentration in human tissue environments that exhibit variable attenuation and propagation path lengths. Our group recently investigated a modulated Acoustic Radiation Force (ARF)-based imaging sequence, which was demonstrated to detect targeted adhesion independent of control measurements. In the present study, this sequence was tested against various experimental parameters to determine feasibility for quantitative measurements of molecular marker concentration. Results demonstrated that measurements obtained from the sequence (residual-to-saturation ratio, Rresid) were independent of acoustic pressure and attenuation (p> 0.13, n = 10)when acoustic pressures were sufficiently low. The Rresid parameter exhibited a linear relationship with measured molecular marker concentration (R2> 0.94). Consequently, feasibility was demonstrated in vitro, for quantification of molecular marker concentration in large vessels using a modulated ARF-based sequence. Moreover, these measurements were independent of absolute acoustic reflection amplitude and used short imaging protocols(3 min) without control measurements.

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“…Zr-89 bevacizumab imaging has been used in the monitoring of tumor angiogenesis. Ultrasound has been used to image VEGF distributions in vivo [60]. Microbubbles can be covalently coupled to VEGF constructs have been used for intravascular imaging [61].…”

Section: Other Molecular Imaging Techniques For Plaque Angiogenesismentioning

confidence: 99%

Magnetic Resonance Molecular Imaging of Plaque Angiogenesis

Winter

Taylor

2012

Curr Cardiovasc Imaging Rep

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Neovascular expansion of the vasa vasorum is an early pathological biomarker of atherosclerosis, preceding endothelial dysfunction. Plaque angiogenesis accompanies intraplaque hemorrhage and plaque rupture, precursors of myocardial infarction and stroke. Molecular imaging of angiogenesis aims to map the expression of neovascular biomarkers on a cellular scale, often utilizing paramagnetic or superparamagnetic MRI contrast agents in order to generate sufficient image enhancement. Both clinically approved extracellular contrast agents and experimental targeted nanoparticles have demonstrated MRI signal enhancement that is proportional to the neovascular density in the vessel wall. Furthermore, targeted contrast agents formulated with anti-angiogenic drugs can be used to quantify drug deposition within the plaque and predict subsequent therapeutic effects. Molecular imaging of plaque angiogenesis has shown promise for interrogating the pathophysiology of atherosclerotic lesions rather than just their physical characteristics, with the ultimate goal of identifying the high-risk plaques that are most likely to cause cardiovascular events.

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Vascular Endothelial Growth Factor Receptor 2-Specific Microbubbles for Molecular Ultrasound Detection of Prostate Cancer in a Rat Model

Abstract: The target-specific contrast agent was superior to the unspecific US contrast agent both with regard to early inflow analysis and specific accumulation in prostate cancer after 20 minutes.

Cited by 37 publications

References 44 publications

Molecular imaging for cancer diagnosis and surgery

Molecular imaging for cancer diagnosis and surgery

Ultrasound-Based Measurement of Molecular Marker Concentration in Large Blood Vessels: A Feasibility Study

Magnetic Resonance Molecular Imaging of Plaque Angiogenesis

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