Vascular endothelial growth factor (<scp>VEGF</scp>) expression in histological endometritis in the bitch: An immunohistochemical study

Praderio, Romina Gisele; Mitacek, María Carla García; Gilead, T; Sota, Rodolfo Luzbel de la; Stornelli, María Alejandra

doi:10.1111/rda.14160

Cited by 4 publications

(2 citation statements)

References 20 publications

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“…It is well known that histological END could be related to subfertility or infertility in the bitch (Gifford et al 2014). A uterine biopsy as a diagnostic tool has been widely used in Veterinary Medicine and has been helpful for the diagnosis of END in mares, cows, and bitches (Kenney and Doig 1986;Mir et al 2013;Madoz et al 2014;Gifford et al 2014;Praderio et al 2019;Praderio et al 2022). In the same way, endometrial cytology has been widely used to diagnose END in cows and mares (Overbeck et al 2013;Madoz et al 2014).…”

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confidence: 99%

The importance of the diestrus stages (early, middle, late) to the diagnosis of the endometrial disease in the bitch

et al. 2023

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The endometrium is the uterine layer wherein embryo implantation and development take place.Complete knowledge of the endometrium's normal histology is critical for advancing the diagnosis of subclinical endometrial diseases. Several researchers studied subclinical endometritis (END) in different species but the cause/s remain unknown. Furthermore, simple and reliable diagnostic tools are still necessary to diagnose subclinical END. In the bitch, diestrus has been selected as the stage of the reproductive cycle to perform the diagnosis of END. This work aimed to perform morphometric and cytological studies in bitches with normal endometrium (NE) and END in diestrus' stages. Forty-ve mixed-breed clinically healthy bitches, in diestrus, were used in this study. Biopsies and cytology were obtained from uterine horns, processed, and examined using a microscope. Samples from NE and END were selected, images acquired, and software for image analysis was used to measure the endometrial thickness (ET), external glandular diameter (EGD), internal glandular diameter (IGD), glandular density, and glandular cell height (CG). Signi cant differences were observed in GC height, EGD, IGD, and serum P 4 concentrations along diestrus. In END, both EGD and IGD were smaller than NE. The current study showed histomorphometric characteristics that appear to be in uenced by P 4 concentrations. In our opinion, it is relevant to consider how far the bitch had progressed into diestrus at the moment of the diagnosis of endometrial disease. Data recorded in the present study could be helpful to clarify cases of bitches with unexplained infertility or subfertility.

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Section: Introductionmentioning

confidence: 99%

The importance of the diestrus stages (early, middle, late) to the diagnosis of the endometrial disease in the bitch

et al. 2023

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View full text Add to dashboard Cite

show abstract

“…It is well known that histological endometritis (END) could be related to subfertility or infertility in the bitch (Gifford et al 2014;Praderio et al 2019). A uterine biopsy as a diagnostic tool has been widely used in Veterinary Medicine (Kenney and Praderio et al 2022). In the same way, endometrial cytology has been widely used to diagnose END in cows and mares (Overbeck et al 2013;Madoz et al 2014); however, is not practical for its use in the bitch (Praderio et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Morphometric changes on the endometrium during diestrus stages in bitches with and without histological endometritis

Praderio

Rearte

Mitacek

et al. 2022

Preprint

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View full text Add to dashboard Cite

The endometrium is a uterine layer essential for achieving optimal embryo implantation and development. Complete knowledge of the endometrium's normal histology is critical for advancing the diagnosis of subclinical endometrial diseases. Several researchers studied subclinical endometritis (END) in different species; however, the cause is unknown, and simple and reliable diagnostic tools are still necessary. In the bitch, diestrus has been selected as the stage of the reproductive cycle to perform diagnostic. This work aimed to perform morphometric and cytological studies in bitches with normal endometrium (NE) and END in the diestrus' different stages. Forty-five mixed-breed bitches, clinically healthy in diestrus, were used in this study. Biopsies and cytology were obtained from uterine horns, processed, and examined using a microscope. Samples from NE and END were selected, photographs were taken, and software for image analysis was used to measure the endometrial thickness (ET), external glandular diameter (EGD), and internal glandular diameter (IGD), glandular density, and glandular cells (GC) height. Significant differences were observed in GC height, EGD, IGD, and serum P4 concentrations along diestrus. In END, EGD and IGD were smaller than NE. The current study showed histomorphometric characteristics that appear to be influenced by P4 concentrations. In our opinion, it is relevant to consider the diestrus stage at the moment of the diagnosis of endometrial disease. Data recorded in the present study could be helpful to bitches with unexplained infertility or subfertility.

show abstract

The importance of the diestrus stages (early, middle, late) to the diagnosis of the endometrial disease in the bitch

Praderio

Rearte

Mitacek

et al. 2022

Preprint

View full text Add to dashboard Cite

The endometrium is the uterine layer wherein embryo implantation and development take place. Complete knowledge of the endometrium's normal histology is critical for advancing the diagnosis of subclinical endometrial diseases. Several researchers studied subclinical endometritis (END) in different species but the cause/s remain unknown. Furthermore, simple and reliable diagnostic tools are still necessary to diagnose subclinical END. In the bitch, diestrus has been selected as the stage of the reproductive cycle to perform the diagnosis of END. This work aimed to perform morphometric and cytological studies in bitches with normal endometrium (NE) and END in diestrus' stages. Forty-five mixed-breed clinically healthy bitches, in diestrus, were used in this study. Biopsies and cytology were obtained from uterine horns, processed, and examined using a microscope. Samples from NE and END were selected, images acquired, and software for image analysis was used to measure the endometrial thickness (ET), external glandular diameter (EGD), internal glandular diameter (IGD), glandular density, and glandular cell height (CG). Significant differences were observed in GC height, EGD, IGD, and serum P4 concentrations along diestrus. In END, both EGD and IGD were smaller than NE. The current study showed histomorphometric characteristics that appear to be influenced by P4 concentrations. In our opinion, it is relevant to consider how far the bitch had progressed into diestrus at the moment of the diagnosis of endometrial disease. Data recorded in the present study could be helpful to clarify cases of bitches with unexplained infertility or subfertility.

show abstract

Vascular endothelial growth factor (VEGF) expression in histological endometritis in the bitch: An immunohistochemical study

Cited by 4 publications

References 20 publications

The importance of the diestrus stages (early, middle, late) to the diagnosis of the endometrial disease in the bitch

The importance of the diestrus stages (early, middle, late) to the diagnosis of the endometrial disease in the bitch

Morphometric changes on the endometrium during diestrus stages in bitches with and without histological endometritis

The importance of the diestrus stages (early, middle, late) to the diagnosis of the endometrial disease in the bitch

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